IFT122 (NM_052989) Human Untagged Clone
CAT＃: SC124458
IFT122 (untagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CED; CED1; FAP80; SPG; WDR10; WDR10p; WDR140 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_052989, the custom clone sequence may differ by one or more nucleotides
ATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAGCACTGTATAAATGACATCGCATTTAAGCCTGATG GAACTCAACTGATTTTGGCTGCCGGAAGCAGATTACTGGTTTATGACACCTCTGATGGCACCTTACTTCA GCCCCTCAAGGGACACAAAGACACTGTGTACTGTGTGGCATATGCGAAGGATGGCAAGCGCTTTGCTTCT GGATCAGCTGACAAAAGCGTTATTATCTGGACATCAAAACTGGAAGGCATTCTGAAGTACACGCACAATG ATGCTATACAATGTGTCTCCTACAATCCTATTACTCATCAACTGGCATCTTGTTCCTCCAGTGACTTTGG GTTGTGGTCTCCTGAACAGAAGTCTGTCTCCAAACACAAATCAAGCAGCAAGATCATCTGCTGCAGCTGG ACAAATGATGGTCAGTACCTGGCGCTGGGGATGTTCAATGGGATCATCAGCATACGGAACAAAAATGGCG AGGAGAAAGTAAAGATCGAGCGGCCGGGGGGCTCCCTCTCGCCAATATGGTCCATCTGCTGGAACCCTTC AAGCCGATGGGAGAGTTTCTGGATGAACAGAGAGAATGAGGATGCCGAGGATGTCATTGTCAACAGATAT ATTCAGGAAATCCCTTCCACTCTGAAGTCAGCAGTGTACAGTAGTCAGGGTAGTGAGGCAGAGGAGGAAG AACCAGAGGAAGAGGACGACAGTCCCAGGGACGACAACTTAGAGGAACGTAATGACATCCTGGCTGTGGC TGACTGGGGACAGAAAGTTTCCTTCTACCAGCTGAGTGGAAAACAGATTGGAAAGGATCGGGCACTGAAC TTTGACCCCTGCTGCATCAGCTACTTTACTAAAGGCGAGTACATTTTGCTGGGGGGTTCAGACAAGCAAG TATCTCTTTTCACCAAGGATGGAGTGCGGCTTGGGACTGTTGGGGAGCAGAACTCCTGGGTGTGGACGTG TCAAGCGAAACCGGATTCCAACTATGTGGTGGTCGGCTGCCAGGACGGCACCATTTCCTTCTACCAGCTT ATTTTCAGCACAGTCCATGGGCTTTACAAGGACCGCTATGCCTACAGGGATAGCATGACTGACGTCATTG TGCAGCACCTGATCACTGAGCAGAAAGTTCGGATTAAATGCAAAGAGCTTGTCAAGAAGATTGCCATCTA CAGAAATCGATTGGCTATCCAACTGCCAGAGAAAATCCTCATCTATGAGTTGTATTCAGAGGACTTATCA GACATGCATTACCGGGTAAAGGAGAAGATTATCAAGAAGTTTGAGTGCAACCTCCTGGTGGTGTGTGCCA ATCACATCATCCTGTGCCAGGAGAAACGGCTGCAGTGCCTGTCCTTCAGCGGAGTGAAGGAGCGGGAGTG GCAGATGGAGTCTCTCATTCGTTACATCAAGGTGATCGGTGGCCCTCCTGGAAGAGAAGGCCTCTTAGTG GGGCTGAAGAATGGACAGATCCTGAAGATCTTCGTGGACAATCTCTTTGCTATCGTCCTGCTGAAGCAGG CCACAGCTGTGCGCTGCTTGGACATGAGTGCCTCCCGTAAGAAGCTGGCCGTGGTAGATGAAAATGACAC TTGCCTGGTGTATGACATCGACACCAAGGAGCTGCTTTTTCAGGAACCAAACGCCAACAGTGTAGCTTGG AACACCCAGTGTGAGGACATGCTCTGCTTCTCGGGAGGAGGCTACCTCAACATCAAAGCCAGCACCTTCC CTGTGCACCGGCAGAAGCTGCAGGGCTTTGTGGTCGGCTACAATGGCTCCAAGATCTTCTGCCTCCATGT CTTCTCCATTTCTGCCGTGGAGGTGCCGCAGTCCGCTCCCATGTACCAGTACCTGGATAGGAAACTGTTC AAGGAAGCCTACCAGATTGCTTGCTTGGGTGTCACAGACACTGATTGGCGTGAACTGGCCATGGAAGCGC TAGAAGGTTTAGATTTTGAAACAGCAAAGAAGGCCTTCATCAGAGTACAAGACCTCCGATATTTAGAGCT CATCAGCAGCATTGAGGAGAGGAAGAAGCGGGGAGAGACCAACAATGACCTGTTTCTGGCAGATGTGTTT TCCTACCAGGGGAAGTTCCATGAGGCCGCCAAACTGTACAAGAGGAGTGGGCACGAGAACCTCGCGCTTG AAATGTACACCGACCTCTGCATGTTTGAGTATGCCAAGGATTTCCTTGGATCTGGAGACCCCAAAGAAAC AAAGATGCTAATCACCAAACAGGCTGACTGGGCCAGAAATATCAAGGAGCCCAAAGCCGCCGTGGAGATG TACATCTCAGCAGGAGAGCACGTCAAGGCCATCGAGATCTGTGGTGACCATGGCTGGGTTGACATGTTGA TCGACATCGCCCGCAAACTGGACAAGGCTGAGCGCGAGCCCCTGCTGCTGTGCGCTACCTACCTCAAGAA GCTGGACAGCCCTGGCTATGCTGCTGAGACCTACCTGAAGATGGGTGACCTCAAGTCCCTGGTGCAGCTG CACGTGGAGACCCAGCGCTGGGATGAGGCCTTTGCTTTGGGTGAGAAGCATCCTGAGTTTAAGGATGACA TCTACATGCCGTATGCTCAGTGGCTAGCAGAGAACGATCGCTTTGAGGAAGCCCAGAAAGCGTTCCACAA GGCTGGGCGACAGAGAGAAGCGGTCCAGGTGCTGGAGCAGCTCACAAACAATGCCGTGGCGGAGAGCAGG TTTAATGATGCTGCCTATTATTACTGGATGCTGTCCATGCAGTGCCTCGATATAGCTCAAGATCCTGCCC AGAAGGACACAATGCTTGGCAAGTTCTACCACTTCCAGCGTTTGGCAGAGCTGTACCATGGTTACCATGC CATCCATCGCCACACGGAAGATCCGTTCAGTGTCCATCGTCCTGAAACTCTTTTCAACATCTCCAGGTTC CTGCTGCACAGCCTGCCCAAGGACACCCCCTCGGGCATCTCTAAAGTGAAAATACTCTTCACCTTGGCCA AGCAGAGCAAGGCCCTCGGTGCCTACAGGCTGGCCCGGCACGCCTATGACAAGCTGCGTGGCCTGTACAT CCCTGCCAGATTCCAAAAGTCCATTGAGCTGGGTACCCTGACCATCCGCGCCAAGCCCTTCCACGACAGT GAGGAGTTGGTGCCCTTGTGCTACCGCTGCTCCACCAACAACCCGCTGCTCAACAACCTGGGCAACGTCT GCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTCTTCCTACGACGTGCTACACCTGGTTGAGTTCTA CCTGGAGGAAGGGATCACTGATGAAGAAGCCATCTCCCTCATCGACCTGGAGGTGCTGAGACCCAAGCGG GATGACAGACAGCTAGAGATTGCAAACAACAGCTCCCAGATTCTGCGGCTAGTGGAGACCAAGGACTCCA TCGGAGATGAGGACCCGTTCACAGCTAAGCTGAGCTTTGAGCAAGGTGGCTCAGAGTTCGTGCCAGTGGT GGTGAGCCGGCTGGTGCTGCGCTCCATGAGCCGCCGGGATGTCCTCATCAAGCGATGGCCCCCACCCCTG AGGTGGCAATACTTCCGCTCACTGCTGCCTGACGCCTCCATTACCATGTGCCCCTCCTGCTTCCAGATGT TCCATTCTGAGGACTATGAGTTGCTGGTGCTTCAGCATGGCTGCTGCCCCTACTGCCGCAGGTGCAAGGA TGACCCTGGCCCATGA >OriGene 5' read for NM_052989 unedited
ACATTTTGTATACGACTACTATAGGCGGCCGCGAAATTCGCACGAGGGCCAAAGTGGCTT GTGGAGTGGCGACCGTTAGTGAGGCGGTTGCTGAGACAGACGCTGAGGCGGGTAGGAGGA GCCCGAGCCGTAAGGGAAGCCGTGATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAG CACTGTATAAATGACATCGCATTTAAGCCTGATGGAACTCAACTGATTTTGGCTGCCGGA AGCAGATTACTGGTTTATGACACCTCTGATGGCACCTTACTTCAGCCCCTCAAGGGACAC AAAGACACTGTGTACTGTGTGGCATATGCGAAGGATGGCAAGCGCTTTGCTTCTGGATCA GCTGACAAAAGCGTTATTATCTGGACATCAAAACTGGAAGGCATTCTGAAGTACACGCAC AATGATGCTATACAATGTGTCTCCTACAATCCTATTACTCATCAACTGGCATCTTGTTCC TCCAGTGACTTTGGGTTGTGGTCTCCTGAACAGAAGTCTGTCTCCAAACACAAATCAAGC AGCAAGATCATCTGCTGCAGCTGGACAAATGATGGTCAGTACCTGGCGCTGGGGATGTTC AATGGGATCATCAGCATACGGGACAAAAATGGCGAGGAGAAAGTAAAGATCGAGCCGGCG GGGGGCTCCCTCTCGCCAATATGTCCATCTGCTTGAACCCTTCAAGAGAGGACCTAATGA CATCCTGTCTGTGGGTTGCTTGGGACAAAAAGTTTCCTTCTCCAACTGAGTGGAAAACAA ATTGGAAAGATCGGGCACTGAATCTTTACCCTGCTGGATAACCTCTTTTATTAAGGGGAG ACATTTTTGTTGGTGGGTGTAAACAAACAAGATATTTTTTAACCAAGAATGGAATGCGCT TGTGACTGTTGGGAGCACAATCTTGGGTGGACGCTTAAACAAACCGTTTCACATGGGTTG GTGGTGTGACAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_052989 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_052989.1, NP_443715.1 |
| RefSeq Size | 4029 bp |
| RefSeq ORF | 3726 bp |
| Locus ID | 55764 |
| UniProt ID | Q9HBG6 |
| Domains | WD40 |
| Gene Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Mutations in this gene cause cranioectodermal dysplasia-1. A related pseudogene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter that isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205180 | IFT122 (Myc-DDK-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
CNY 12960.00 |
|
| RC205180L1 | Lenti-ORF clone of IFT122 (Myc-DDK-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
CNY 15360.00 |
|
| RC205180L2 | Lenti-ORF clone of IFT122 (mGFP-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
CNY 12540.00 |
|
| RC205180L3 | Lenti-ORF clone of IFT122 (Myc-DDK-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
CNY 12540.00 |
|
| RC205180L4 | Lenti-ORF clone of IFT122 (mGFP-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
CNY 12540.00 |
|
| RG205180 | IFT122 (tGFP-tagged) - Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 2 |
CNY 11690.00 |
