P15RS (RPRD1A) (BC010136) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cyclin-dependent kinase 2B-inhibitor-related protein; Cyclin-dependent kinase inhibitor 2B-related protein (p15INK4B-related protein); FLJ10656; HsT3101; MGC19513; P15RS; regulation of nuclear pre-mRNA domain containing 1A
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC010136 edited CTTGCTGGTTTGCGGCCGGTCTTGGATGAAGCGGCGGCCGTGGTGAGAGCGTGGGGAAGG GTGGGGTGAGGGGGCGAGGCCGCAGCTAGGGCGGCGAAACTCTCCTCCCCTCGGCCCCAC CGCGTGGGACGGCGTGAACGTGGTGTCGGAGGGATGTCAGCCTTCTCTGAGGCGGCGCTG GAGAAGAAGCTGTCGGAGTTGAGCAACTCGCAGCAGAGCGTGCAGACCTTGTCCCTGTGG CTCATTCACCACCGTAAACACTCGCGTCCCATCGTCACCGTGTGGGAGCGGGAGCTGCGG AAAGCCAAACCAAACAGGAAGCTTACTTTTCTCTACCTAGCCAATGATGTCATACAGAAC AGCAAGAGGAAGGGGCCAGAGTTTACAAAAGATTTTGCACCAGTTATAGTGGAGGCTTTT AAGCATGTTTCAAGTGAAACTGATGAAAGTTGTAAGAAGCACCTTGGAAGAGTGTTATCT ATTTGGGAAGAAAGGTCTGTTTATGAAAATGATGTATTAGAACAACTTAAACAAGCTCTG TATGGTGATAAGAAGCCTAGGAAGCGAACTTATGAACAGATAAAGGTGGATGAAAATGAA AACTGTTCCTCTCTGGGATCTCCAAGTGAACCACCACAGACTCTAGATCTCGTTAGAGCA TTACAAGATCTGGAAAATGCAGCCTCAGGTGATGCAGCAGTTCATCAGAGGATAGCTTCT TTACCTGTTGAAGTCCAAGAAGTATCTCTATTAGATAAAATAACAGATAAAGAATCTGGA GAAAGGCTTTCCAAAATGGTAGAGGATGCGTGTATGTTGCTGGCAGATTACAATGGCAGA TTGGCGGCAGAAATAGATGATAGAAAGCAACTCACTCGAATGTTAGCAGATTTTCTTCGT TGTCAAAAGGAAGCCCTTGCAGAGAAAGAGCATAAATTGGAAATATTGGACATGGGCGTA TCTTCTAACGATGTCGTCACCATTAGTATATTCTTCCTGCTAAAAATAAATATCAAACCC TGAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010136 unedited NGAACTTGTCACATTTGTAAACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCTTG CTGGTTTGCGGCCGGTCTTGGCTGAAGCGGCGGCCGTGGTGAGAGCGTGGGGAAGGGTGG GGTGAGGGGGCGAGGCCGCAGCTAGGGCGGCGAAACTCTCCTCCCCTCGGCCCCACCGCG TGGGACGGCGTGAACGTGGTGTCGGAGGGATGTCAGCCTTCTCTGAGGCGGCGCTGGAGA AGAAGCTGTCGGAGTTGAGCAACTCGCAGCAGATCGTGCAGACCTTGTCCCTGTGGCTCA TTCACCACCGTAAACACTCGCGTCCCATCGTCACCGTGTGGGAGCGGGAGCTGCGGAAAG CCAAACCAAACAGGAAGCTTACTTTTCTCTACCTAGCCAATGATGTCATACAGAACAGCA AGAGGAAGGGGCCAGAGTTTACAAAAGATTTTGCACCAGTTATAGTGGAGGCTTTTAAGC ATGTTTCAAGTGAAACTGATGAAAGTTGTAAGAAGCACCTTGGAAGAGTGTTATCTATTT GGGAAGAAAGGTCTGTTTATGAAAATGATGTATTAGAACAACTTAAACAAGCTCTGTATG GTGATAAGAAGCCTAGGAAGCGAACTTATGAACAGATAAAGGTGGATGAAAATGAAAACT GTTCCTCTCTGGGATCTCCAAGTGAACCACCACAGACTCTAGATCTCGTTAGAGCATTAC AAGATCTGGAAAATGCAGCCTCAGGTGATGCAGCAGTTCATCAGAAGATAGCTTCTTTAC CTGTTGAAGTCCAAGAAGTATCTCTATTAGATAAAATACAGATAAAGAATCTGGAGAAAG GCTTTCCAAATGGTAAAAGATGCCTGTATGTTGCTGGCAT
Restriction Sites	Please inquire
ACCN	BC010136
Insert Size	1053 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010136.2, AAH10136.1
RefSeq Size	1044 bp
Locus ID	55197
Gene Summary	This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Dec 2014]

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