NELFE (BC011600) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC011600 edited GGCACGAGGGGTTGCCGCCCCCTCGGGAGCCACCATGTTGGTGATACCCCCCGGACTGAG CGAGGAAGAGGAGGCTCTGCAGAAGAAATTCAACAAGCTCAAGAAAAAGAAAAAGGCATT GCTGGCTCTGAAGAAGCAAAGTAGCAGCAGCACAACCAGCCAAGGTGGTGTCAAACGCTC ACTATCAGAGCAGCCTGTCATGGACACAGCCACAGCAACAGAGCAGGCAAAGCAGCTGGT GAAGTCAGGAGCCATCAGTGCCATCAAGGCTGAGACCAAGAACTCAGGCTTCAAGCGTTC TCGAACCCTTGAGGGGAAGTTAAAGGACCCCGAGAAGGGACCAGTCCCCACTTTCCAGCC GTTCCAGAGGAGCATATCTGCTGATGATGACCTGCAAGAGTCATCCAGACGTCCCCAGAG GAAATCTCTGTATGAGAGTGATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGA GGGCCCAGGGGCTGGTGATGGTCCCCCTCGAAGCTTTGACTGGGGCTATGAAGAACGCAG TGGTGCCCACTCCTCAGCCTCCCCTCCCCGAAGCCGCAGCCGGGACCGCAGCCATGAGAG GAACCGGGACAGAGACCGAGAGGGTCCTTTCCGCAGGTCGGATTCATTCCCTGAACGGCG AGCCCCTAGGAAAGGGAATACTCTCTATGTATATGGAGAAGACATGACACCCACCCTTCT CCGTGGGGCCTTCTCTCCTTTTGGAAACATCATTGACCTCTCCATGGACCCACCCAGAAA CTGTGCCTTCGTCACCTATGAAAAGATGGAGTCAGCAGATCAGGCCGTTGCTGAGCTCAA CGGGACCCAGGTGGAGTCTGTACAGCTCAAAGTCAACATAGCCCGAAAACAGCCCATGCT GGATGCCGCTACTGGCAAGTCTGTCTGGGGCTCCCTCGCTGTCCAGAACAGCCCTAAGGG TTGCCACCGGGACAAGAGGACCCAGATTGTCTACAGTGATGACGTCTACAAGGAAAACCT TGTGGATGGCTTCTAGGGAACAGAGCTGGATTCCTTGTGCCTCATATGCCCCAATGCTGG TCTCAGTAAAACACTGAGGTGGAAGCTTAAAAAAAAAGGAGCGTCTCTGCCCGGCCTCCC CGTCTGAGAAGTGAGGAAACCCTCTGCCTGGCAACCGCCCCGTCTGAGAAGTGAGGAGCC CCTCCGTCCGGCAGCCACCCCGTCTGGGAAGTGAGGAGCGTCTCCGCCCGGCAGCCACCC CGACCGGGAGGGAGGTGGGGGGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCG GGAGGTGAGGGGCTCCTCTGCCCGGCCGCCCCTACTGGGAAGTGAGGAGCCCCTCTGCCC GGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGATCAGCCCCCCGCCTGGCCAGCCGCC CCGTCCGGGAGGTGAGGGGCGCCTCTGCCCGGCCGCCCCTACTGGGAAGTGAGGAGCCCC TCTGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCC AGCCGCCCCATCCGGGAGGGAGGTGGGGGGGTCAGCCCTCCGCCCGGCCAGCCGCCCCGT CCGGGAGGGAGGTGGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGGA GGTGGGGGGATCAGCCCCCCGCCTGGCCAGCCACCCCGTCCGGGAGGTGAGGGGCGCCTC TGCCCGGCCGCCCCTACTGGGAAGTGAGGACCCCTCTGCCTGGCCAGCCGCCCCGTCCGG GAGGGTTGGGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCATCCGGGAGGTGAGGGG CGCTTCTGCCCGGCCGCCCCTACTGGGAAATGAGGAGCCCCTCTGCCCGGCCACGACCCC GTCTGGGAGGTGTGCCCAGCGGCTCATTGGGGATGGGCCATGATGACAATGGCGGTTTTG TGGAATAGAAAGGCGGGAAGAGTGGGGAAAAAATTGAGAAATCGGATGGTTGCCGGGTCT CTGTGGATAGAAGTAGACATGGGAGACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTT CTGCCTTGGGATCCTGTTGATCTGTGACCTTATCCCCAACCCTGTGCTCTCTGAAACATG TGCTGTGTCCACTCAGGGTTAAATGGATTAAGGGCGGTGCAAGATGTGCTTTGTTAAACA GATGCTTGAAGGCAGCATGCTCGTTAAGAGTCATCACCACTCCCTAATCTTAAGTACCCA GGGACACAAACACTGCGGAAGGCCGCAGGGTCCTCTGCCTAGGAAAACCAGAGACCTTTG TTCACTTGTTTATCTACTGACCTTCCCTCCACTATTGTCCTATGACCCTGCCAAATCCCC CTCTGCGAGAAACACCCAAGAATGATCAATAAAATAAATAAATAAATAAATAAATAATAA AAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011600 unedited AGGTTCAAATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACGGGGGTTGCCGC CCCCTCGGGAGCCACCATGTTGGTGATACCCCCCGGACTGAGCGAGGAAGAGGAGGCTCT GCAGAAGAAATTCAACAAGCTCAAGAAAAAGAAAAAGGCATTGCTGGCTCTGAAGAAGCA AAGTAGCAGCAGCACAACCAGCCAAGGTGGTGTCAAACGCTCACTATCAGAGCAGCCTGT CATGGACACAGCCACAGCAACAGAGCAGGCAAAGCAGCTGGTGAAGTCAGGAGCCATCAG TGCCATCAAGGCTGAGACCAAGAACTCAGGCTTCAAGCGTTCTCGAACCCTTGAGGGGAA GTTAAAGGACCCCGAGAAGGGACCAGTCCCCACTTTCCAGCCGTTCCAGAGGAGCATATC TGCTGATGATGACCTGCAAGAGTCATCCAGACGTCCCCAGAGGAAATCTCTGTATGAGAG TGATCGACTTCGAGAACTAGGACCAGATGGAGAAGAGGCAGAGGGCCCAGGGGCTGGTGA TGGTCCCCCTCGAAGCTTTGACTGGGGCTATGAAGAACGCAGTGGTGCCCACTCCTCAGC CTCCCCTCCCCGAAGCCGCAGCCGGGACCGCAGCCATGAGAGGAACCGGGACAGAGACCG AGAGGGTCCTTTCCGCAGGTCGGATTCATTCCCTGAACGGCGAGCCCCTAGGAAAGGGAA TACTCTCTATGTATATGGGAGAGACATGACACCCACCCTTCTCCGTGGGGCCTTCTCNTC TTTTGGAAACATCATTGACCTCTCCATGGACCCACCCAGAAACTGTGCCTTCGTCACCTA TGAAAAGATGGAGTCAGCAGATCAGGGCCGTGCTGAGCTCACGGGACCCAGTGGAGTCTG TCAGCTCAAGTC
Restriction Sites	Please inquire
ACCN	BC011600
Insert Size	2478 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC011600.1, AAH11600.1
RefSeq Size	2478 bp
Locus ID	7936
Protein Families	Transcription Factors
Gene Summary	The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

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