RHBDL2 (BC013103) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC16997; OTTHUMP00000000546; rhomboid (veinlet, Drosophila)-like 2; rhomboid, veinlet-like 2 (Drosophila); rhomboid-related protein 2; RRP2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC013103 edited AAGGAGGAGCGCCTGGCAGAGCGGGATCTTCAGGGCAGCGGGCAACCCCTTGGCCCAGGA AGCCTGGAACGCAAGGACCGGAGGGCGTGGGCTGGGACGCCCCTACGTTGGTCTTTCAGG GAAAGGCCTTGGAAAGCAGTCGTTGCGCCAGACAGCCCAGGGAAGAGCGGCAGCCTGAGG ACCTAGGGCCACCTGCTGTTCCCTGGGATTCATGTCCTTCTGGGGAGGAGGGAGGACCCA GGACAATGGCTGCTGTTCATGATCTGGAGATGGAGAGCATGAATCTGAATATGGGGAGAG AGATGAAAGAAGAGCTGGAGGAAGAGGAGAAAATGAGAGAGGATGGGGGAGGTAAAGATC GGGCCAAGAGTAAAAAGGTCCACAGGATTGTCTCAAAATGGATGCTGCCCGAAAAGTCCC GAGGAACATACTTGGAGAGAGCTAACTGCTTCCCGCCTCCCGTGTTCATCATCTCCATCA GCCTGGCCGAGCTGGCAGTGTTTATTTACTATGCTGTGTGGAAGCCTCAGAAACAGTGGA TCACGTTGGACACAGGCATCTTGGAGAGTCCCTTTATCTACAGTCCTGAGAAGAGGGAGG AAGCCTGGAGGTTTATCTCATACATGCTGGTACATGCTGGGTAAGCAATGATAGTTAAGC CCCTGGTATCAGAGGTGATTATATCATTGTAACCTCTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC013103 unedited AGTCAGATTTTGTAATACGACTCACTATAGGGNCGGCACGCGNAATTCCCGGGGAATCGT CGACCCTTCGTCCGAAGGAGAGCGCCTGGCAGAGCGGGATCTTCATGGCAGCGGGCAACC CCTTGGCCCATGAAGCCTGGAACGCAAGGACCGGAGGGCGTGGGCTGGGACGCCCCTACG TTGGTCTTTCAGGGAAAGGCCTTGGAAAGCAGTCGTTGCGCCAGACAGCCCATGGAAGAG CGGCAGCCTGAGGACCTATGGCCACCTGCTGTTCCCTGGGATTCATGTCCTTCTGGGGAG GAGGGAGGACCCAGGACAATGGCTGCTGTTCATGATCTGGAGATGGAGAGCATGAATCTG AATATGGGGAGAGAGATGAAAGAAGAGCTGGAGGAAGAGGAGAAAATGAGAGAGGATGGG GGAGGTAAAGATCGGGCCAAGAGTAAAAAGGTCCACAGGATTGTCTCANAATGGATGCTG CCCGAAAAGTCCCGAGGAACATACTTGGAGAGAGCTAACTGCTTCCCGCCTCCCGTGTTC ATCATCTCCATCAGCCTGGCCGAGCTGGCAGTGTTTATTTACTATGCTGTGTGGAAGCCT CAGAAACAGTGGATCACGTTGGACACAGGCATCTTGGAGAGTCCCTTTATCTACAGTCCT GAGAAGAGGGAGGAAGCCTGNNAGTTTATCTCATACATGCTGGTACATGCTGGGTAAAGC ATGATAGTTAAGCCCCTGGTATCAGAGGTGATTATATCATTGTAACCTCTAAAAAAAAAA AAAAAGGCNGCCCGCGTCATAGCTGTTTCCTGAACAGATCCCGGGTAGCATNCCNNTGTG ACCCTCCNCAGTGGCCTCTCCTGGCCCNTGGAAAGTTGCACTCCAGTGCCCCAACAACCT TGTCCTAAATAAATTAAGTTGCATCATTTTTGTCTGACTAAGTGTCCTT
Restriction Sites	Please inquire
ACCN	BC013103
Insert Size	712 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013103.1, AAH13103.1
RefSeq Size	712 bp
RefSeq ORF	366 bp
Locus ID	54933
Protein Families	Protease, Transmembrane
Gene Summary	The protein encoded by this gene is a member of the rhomboid family of integral membrane proteins. This family contains proteins that are related to Drosophila rhomboid protein. Members of this family are found in both prokaryotes and eukaryotes and are thought to function as intramembrane serine proteases. The encoded protein is thought to release soluble growth factors by proteolytic cleavage of certain membrane-bound substrates, including ephrin B2 and ephrin B3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]

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