PDF (NM_022341) Human Untagged Clone
CAT＃: SC122932
PDF (untagged)-Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein
CNY 3990.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_022341 edited
CATGGCCCGGCTGTGGGGCGCGCTGAGTCTTCGGCCACTGTGGGCGGCCGTGCCGTGGGG CGGGGCGGCAGCCGTCGGTGTCCGGGCTTGCAGCTCCACGGCCGCCCCGGACGGCGTCGA GGGCCCGGCGCTGCGGCGCTCCTATTGGCGCCACCTGAGGCGTCTGGTGCTGGGTCCTCC CGAACCGCCGTTCTCGCACGTGTGCCAAGTCGGGGACCCGGTGCTGCGCGGCGTGGCGGC CCCGGTGGAGCGGGCGCAGCTAGGCGGGCCCGAGCTGCAGCGGCTGACGCAACGGCTGGT CCAGGTGATGCGGCGGCGGCGCTGCGTGGGCCTAAGCGCGCCGCAGCTGGGGGTGCCGCG GCAGGTGCTGGCGCTGGAGCTCCCCGAGGCGCTGTGTCGGGAGTGCCCGCCCCGCCAGCG CGCGCTCCGCCAAATGGAGCCCTTCCCCCTGCGCGTGTTCGTGAACCCCAGCCTGCGAGT GCTTGACAGCCGCCTGGTCACCTTTCCCGAGGGCTGCGAGAGCGTCGCCGGCTTCCTGGC CTGCGTGCCCCGCTTCCAGGCGGTGCAGATCTCAGGGCTGGACCCCAATGGAGAACAGGT GGTGTGGCAGGCGAGCGGGTGGGCAGCCCGCATCATCCAGCACGAGATGGACCACCTGCA GGGCTGCCTGTTTATTGACAAAATGGACAGCAGGACGTTCACAAACGTCTATTGGATGAA GGTGAATGACTAAAGCTTTGCTACTGGGGCTGAGGATTCCGGATACCAAGACGCAAACAC TTTCACTTTGAGCTGGGCAAATCTTACTTGGCATCAACTTGGATGGCTCGCATATGACAG GAAACTGGATTGCCAAGGCATGGCAGACTGAGCTGGAGGAAGATGTCAGAAATGTTTGCC CTGAAATCAGTTACGGACAAAATGTTGGCTACAACTTGAGGAGAAAAATCACCCCCAAAG GAGTGGACATTTCCTAACAATTCTGTATGGAGGAAGGTGGGGTAATTGCATTCGTCTGCA GTAGACACGAGTTCCTCGGACCTGTATAATCTCCCAAAGCCAAGGTTTGGTATTAATGTA GTCCCCAAATACCTGAAAGCTGTCTTTAAAAATGCAGGTAAGCATGTGACTGGCAAAAAA AAAAAAAAAAAAAAAA >OriGene 5' read for NM_022341 unedited
GGGCCGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATCCCGGGATATCGTCG ACCCACGCGTCCGCATGGCCCGGCTGTGGGGCGCGCTGAGTCTTCGGCCACTGTGGGCGG CCGTGCCGTGGGGCGGGGCGGCAGCCGTCGGTGTCCGGGCTTGCAGCTCCACGGCCGCCC CGGACGGCGTCGAGGGCCCGGCGCTGCGGCGCTCCTATTGGCGCCACCTGAGGCGTCTGG TGCTGGGTCCTCCCGAACCGCCGTTCTCGCACGTGTGCCAAGTCGGGGACCCGGTGCTGC GCGGCGTGGCGGCCCCGGTGGAGCGGGCGCAGCTAGGCGGGCCCGAGCTGCAGCGGCTGA CGCAACGGCTGGTCCAGGTGATGCGGCGGCGGCGCTGCGTGGGCCTAAGCGCGCCGCAGC TGGGGGTGCCGCGGCAGGTGCTGGCGCTGGAGCTCCCCGAGGCGCTGTGTCGGGAGTGCC CGCCCCGCCAGCGCGCGCTCCGCCAAATGGAGCCCTTCCCCCTGCGCGTGTTCGTGAACC CCAGCCTGCGAGTGCTTGACAGCCGCCTGGTCACCTTTCCCGAGGGCTGCGAGAGCGTCG CCGGCTTCCTGGCCTGCGTGCCCCGCTTCCAGGCGGTGCAGATCTCAGGGCTGGACCCCA ATGGAGAACAGGTGGTGTGGCANGCGAGCGGGTGGGCAGCCCGCATCATCCAGCACGAGA TGGACCACCTGCNAGGCTGCCTGTTTATTGANCAAATGGACAGCAGGACGTTCACAAACG TCTATTGGATGAAGGTGAATGACTAAAGCTTTGCTACTGGGGCTNGAGATTCCGGATACC AAGACGCAACACTTTCACTTTGAGCTGGGCAATCTTACTTGGCATCAACTGGGATG |
| Restriction Sites | Please inquire |
| ACCN | NM_022341 |
| Insert Size | 1156 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022341.1, NP_071736.1 |
| RefSeq Size | 1180 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 64146 |
| UniProt ID | Q9HBH1 |
| Gene Summary | Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205788 | PDF (Myc-DDK-tagged)-Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
|
| RC205788L1 | Lenti ORF clone of Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 4800.00 |
|
| RC205788L2 | Lenti ORF clone of Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RC205788L3 | Lenti ORF clone of Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205788L4 | Lenti ORF clone of Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG205788 | PDF (tGFP-tagged) - Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein |
CNY 4000.00 |
