RGNEF (ARHGEF28) (BC012946) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp686P12164; FLJ21817; KIAA1998; Rho-guanine nucleotide exchange factor
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC012946 edited ATCTTTTGGCTCGACGCCGAGGAATTCCAGAATGCATTCTGTTGGTCACTCAGCGTATTA CAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACACAAAGGAAAGAACTGAGGAAC ATAAAGACTTACGCAAAGCGCTTTGCTTAATTAAAGACATGATTGCAACAGTGGATTTAA AAGTCAATGAATATGAGAAAAACCAAAAATGGCTTGAGATCCTAAATAAGATTGAAAACA AAACATACACGAAGCTCAAAAATGGACATGTGTTTAGGAAGCAGGCACTGATGAGTGAAG AAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAACTGCTACAGGTCGTTTCAAAG ATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTTTACAAGAAAAAGACCAGAAAT ACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTTCCCTTCAAAAGCTTATTGCTA GAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCAGTGCTTCATCTGCTGGTCCTG AGATGTATGAAATTCACACCAATTCCAAGGAGGAACGCAATAACTGGATGAGACGGATCC AGCAGGCTGTAGAAAGTTGTCCTGAAGAAAAAGGGGGAAGGACAAGTGAATCTGATGAAG ACAAGAGGAAAGCTGAAGCCAGAGTGGCCAAAATTCAGCAATGTCAAGAAATACTCACTA ACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGCTGCATATCTATGCTGAACTTG GAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCCCACCTCCTTATTAAACCTGACC CAGGCGAGCCTCCCCAGGCAGCCTCATTACTGGCAGCAGCACTGAAAGAAGCTGAGAGCC TACAAGTTGCAGTGAAGGCCTCACAGATGGGCGCCGTGAGTCAATCATGTGAGGACAGTT GTGGAGACTCTGTCTTGGCGGACACACTCAGTTCTCATGATGTACCAGGATCACCGACTG CCTCATTAGTCACAGGAGGGAGAGAAGGAAGAGGCTGTTCGGATGTGGATCCCGGGATCC AGGGTGTGGTAACCGACTTGGCCGTCTCTGATGCAGGGGAGAAGGTGGAATGTAGAAATT TTCCAGGTTCTTCACAATCAGAGATTATACAAGCCATACAGAATTTAACCCGTCTCTTAT ACAGCCTTCAGGCCGCCTTGACCATTCAGGACAGCCACATTGAGATCCACAGGCTGGTTC TCCAGCAGCAGGAGGGCCTGTCTCTCGGCCACTCTATCCTCCGAGGCGGCCCCTTGCAGG ACCAGAAGTCTCGCGACGCGGACAGGCAGCATGAGGAGCTGGCCAATGTGCACCAGCTTC AGCACCAGCTCCAGCAGGAGCAGCGGCGCTGGCTGCGCAGGTGTGAGCAGCAGCAGCGGG CGCAGGCGACCAGGGAGAGCTGGCTGCAGGAGCGGGAGCGGGAGTGCCAGTCGCAGGAGG AGCTGCTGCTGCGGAGCCGGGGCGAGCTGGACCTCCAGCTCCAGGAGTACCAGCACAGCC TGGAGCGGCTGAGGGAGGGCCAGCGCCTGGTGGAGAGGGAGCAGGCGAGGATGCGGGCCC AGCAGAGCCTGCTGGGCCACTGGAAGCACGGCCGGCAGAGGAGCCTGCCCGCGGTGCTCC TTCCGGGTGGCCCCGAGGTAATGGAACTTAACCGATCTGAGAGTTTATGTCATGAAAACT CATTCTTCATCAATGAAGCTTTAGTACAAATGTCATTTAACACTTTCAACAAACTGAATC CATCAGTTATCCATCAGGATGCCACTTACCCTACAACTCAATTTCATTCTGACTTGGTGA GGACTAGTGAACATCAAGTAGACCTCCTGGTGGACCCTTCTCAGCCTTTGAATGTCAGTC ACAAACTGTGGACAGCCGCTGGTTCCGGCCATCAGATACTTCCTTTCCATGAAAGCAGCA AGGATTCTTGTAAAAATGGTAATTAACACTTTAAACATCATCTGTATAGTTTGAACAAGA AGTTGTCCCTCTGAATGGTTGGCTCTTGTGTAGAGTGAATCAAAGTCCTCCCTAGCATGG GAATAAATCGGTACTGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012946 unedited NNGGCGGGGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGNAATCCANATGCATTC TGTTGGTCACTCAGCGTATTACAAAATACCCTGTCTTGGTGGAAAGGATATTGCAGTACA CAAAGGAAAGAACTGAGGAACATAAAGACTTACGCAAAGCGCTTTGCTTAATTAAAGACA TGATTGCAACAGTGGATTTAAAAGTCAATGAATATGAGAAAAACCAAAAATGGCTTGAGA TCCTAAATAAGATTGAAAACAAAACATACACGAAGCTCAAAAATGGACATGTGTTTAGGA AGCAGGCACTGATGAGTGAAGAAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAA CTGCTACAGGTCGTTTCAAAGATATCCTAGCTCTACTTCTAACTGATGTGCTGCTCTTTT TACAAGAAAAAGACCAGAAATACATCTTTGCAGCCGTTGATCAGAAGCCATCAGTTATTT CCCTTCAAAAGCTTATTGCTAGAGAAGTTGCTAATGAGGAGAGAGGAATGTTTCTGATCA GTGCTTCATCTGCTGGTCCTGAGATGTATGAAATTCACACCAATTCCAAGGAGGAACGCA ATAACTGGATGAGACGGATCCAGCAGGCTGTAGAAAGTTGTCCTGAAGAAAAAGGGGGAA GGACAAGTGAATCTGATGAAGACAAGAGGAAAGCTGAAGCCAGAGTGGCCAAAATTCAGC AATGTCAAGAAATACTCACTAACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGC TGCATATCTATGCTGAACTTGGAGAACTGAGCGGATTTGAGGACGTCCATCTAGAGCCCA CCTCCTTNATTAACCTGACCCAGCG
Restriction Sites	Please inquire
ACCN	BC012946
Insert Size	2151 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012946.1, AAH12946.1
RefSeq Size	2151 bp
Locus ID	64283
Gene Summary	This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

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