ATP1A4 (BC028297) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP1A1; ATP1AL2; ATPase, Na+/K+ transporting, alpha polypeptide-like 2; MGC25056; Na+/K+ -ATPase alpha 4 subunit; Na+/K+ ATPase, alpha-D polypeptide; Na+/K+ ATPase 4; Na,K-ATPase subunit alpha-C; OTTHUMP00000029890; sodium/potassium-transpo; sodium pump 4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC028297 edited CACCAGCCCAGCCAGAAGCAAGTCCCAGCCCCCAGCCCTCCCCTGGCCTACCTTTTGGGG CCCTTCTCTGAACCAGGCTCCCCTGTCCTGCAACTCTGTCATTCACAGGGATGATCCAGG CTCTGGCTGGATTCTTTACCTACTTTGTAATCCTGGCTGAGAATGGTTTTAGGCCTGTTG ATCTGCTGGGCATCCGCCTCCACTGGGAAGATAAATACTTGAATGACCTGGAGGACAGCT ACGGACAGCAGTGGACCTATGAGCAACGAAAAGTTGTGGAGTTCACATGCCAAACGGCCT TTTTTGTCACCATCGTGGTTGTGCAGTGGGCGGATCTCATCATCTCCAAGACTCGCCGCA ACTCACTTTTCCAGCAGGGCATGAGAAACAAAGTCTTAATATTTGGGATCCTGGAGGAGA CACTCTTGGCTGCATTTCTGTCCTACACTCCAGGCATGGACGTGGCCCTGCGAATGTACC CACTCAAGATAACCTGGTGGCTCTGTGCCATTCCCTACAGTATTCTCATCTTCGTCTATG ATGAAATCAGAAAACTCCTCATCCGTCAGCACCCGGATGGCTGGGTGGAAAGGGAGACGT ACTACTAAACTCAGCAGATGAAGAGCTTCATGTGACACAGGGGTGTTGTGAGAGCTGGGA TGGGGCCAGAGATTATAAGTTTGACACAACATCTGAGACACTAGGATGAATTATCTTGGA TGAGAAAGATGGGCAATCCTGGGCTGGCTTGAGGGAATCATGGGCAGAGGATGAGGTGGG CTGAAGGGAAGCCCAGCCTGCATCTAGCTGGAGCCCCGCAGGGAGGGGCATGGTCCTGCT GAATCCCGTAGCCAGTCTAGACAGTAAATGTCTGGAAAAGCCCTCAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC028297 unedited NGGTTTTGATTTGTAAACGACTTATATAGGCGGCCGCGCAATCCCGGGATATCGTCGACC CACGCGTCCGCACCAGCCCAGCCAGAAGCAAGTCCCAGCCCCCAGCCCTCCCCTGGCCTA CCTTTTGGGGCCCTTCTCTGAACCAGGCTCCCCTGTCCTGCAACTCTGTCATTCACAGGG ATGATCCAGGCTCTGGCTGGATTCTTTACCTACTTTGTAATCCTGGCTGAGAATGGTTTT AGGCCTGTTGATCTGCTGGGCATCCGCCTCCACTGGGAAGATAAATACTTGAATGACCTG GAGGACAGCTACGGACAGCAGTGGACCTATGAGCAACGAAAAGTTGTGGAGTTCACATGC CAAACGGCCTTTTTTGTCACCATCGTGGTTGTGCAGTGGGCGGATCTCATCATCTCCAAG ACTCGCCGCAACTCACTTTTCCAGCAGGGCATGAGAAACAAAGTCTTAATATTTGGGATC CTGGAGGAGACACTCTTGGCTGCATTTCTGTCCTACACTCCAGGCATGGACGTGGCCCTG CGAATGTACCCACTCAAGATAACCTGGTGGCTCTGTGCCATTCCCTACAGTATTCTCATC TTCGTCTATGATGAAATCAGAAAACTCCTCATCCGTCAGCACCCGGATGGCTGGGTGGAA AGGGAGACGTACTACTAAACTCAGCAGATGAAGAGCTTCATGTGNACACAGGGGTGTTGT GAGAGCTGGGATGGGGCCAGAGATTATAAGTTTGACACAACATCTGAGACACTAGGATGA ATTATCTTGGATGAGAAAGATGGGCAATCCTGGGCTGGCTTGAGGGAATCATGGGCAGAG GATGAANGTGGGCTGAANGGNAAGCCCAGCCTGCATCTAGCTGGAGCCCCGCAGGNAGGG GCATGGTCCTGCTGAATCCCGTAGCCAGTC
Restriction Sites	Please inquire
ACCN	BC028297
Insert Size	951 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028297.1, AAH28297.1
RefSeq Size	951 bp
RefSeq ORF	498 bp
Locus ID	480
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cardiac muscle contraction
Gene Summary	The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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