ELL (BC010010) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C19orf17; ELL1; MEN; PPP1R68
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC010010 edited GGCACGAGGGATGGGTCTGCCTGCTCTGGGGGTGCAGGCTCTGCAGACACCCCAATTGCT GCTCTGTTGCTGTGCCTAGTCTAGTGTCCCCCAAAGCATCGGGGGGCCACGCAAGTGTGG GTGTGTGAGTGTGCGTATGTGGGGACCCCCCAGGGCATGGTGGGGTCTGAGGCGTGTGTT CCCATTTAGGGCTGAATGAAGCCATGACTTGGAAAGGTGGTGGGGGGTGGATGGCGGCTG TGACTCAGGGCCCAGGGATCACATGGGGGTCACTGCTGCCATCAGCAGTCAGGCAGGGAG AACAGAAATGCAAAAATACAGATGTGTCTATTTTTCTAAACTGGGGGGTGGAGGGGTGCC TCCTGACAGCTTCCAAAGAGCTGACTGTGGAGGGCCCACAGTGTATCCCACCCCTGCCCG AGAGTCTCTCTCCTTTGGGACCCCCGGGTTCCCAGAGCTCCGAGGAAACTGATCCTTCAT GTTTCAATAGCTGTTGTTGTTTTTCTCTTTGGGCCGGGCCGGGCCGGGGAAGGGCCGAGC AGGCTCCAGGAGGCCTCCAGAGTCAGCTGGACCTTGCTGTGTCGCCCGCAGGCAGCCCTG CAGACACCGGTGAGGTGGGATGATTTCAGGAGGTCCCAGCCCGGGACTGCCCGCCCCTCT GCCCTGGGAACCAGTCCAAGGGGCTGTCAGTTGAATTTGCCTTTTTGTTGTTGTTGTTGT TTTTGTTGTTTATTTGTTCTCCTTTTAAGGAATTATGAAGCTAAGAAAAGTTTTGTGTTT TGGGGGTTGGTGGACAAAGTTCTAGCTGATTAAATATGGTCTAACTTATTGATACTATTT TTTTTCTTTTAATGTTGTACTGTGGAGAAAAACTATTTTGAGCCATGGGGTTGGTGTTTA CAAGAACTTTCCACTTTTGCCAAAATGTTACAATTGAAAGGCATCTGCGTCTTCAGTTTC CTGATATTTTCTTAAAAGATCTAGTGTCCTTTTTGTACACTAAACAGGTGAATGCTGACT TTTTTCATTCTCCAATAAATTTCACTGAACTGAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010010 unedited CGTCACAATTGTATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGGATGGGTCTG CCTGCTCTGGGGGTGCAGGCTCTGCAGACACCCCAATTGCTGCTCTGTTGCTGTGCCTAG TCTAGTGTCCCCCAAAGCATCGGGGGGCCACGCAAGTGTGGGTGTGTGAGTGTGCGTATG TGGGGACCCCCCAGGGCATGGTGGGGTCTGAGGCGTGTGTTCCCATTTAGGGCTGAATGA AGCCATGACTTGGAAAGGTGGTGGGGGGTGGATGGCGGCTGTGACTCAAGGCCCAGGGAT CACATGGGGGTCACTGCTGCCATCAGCAGTCAGGCAGGGAGAACAGAAATGCAAAAATAC AGATGTGTCTATTTTTCTAAACTGGGGGGTGGAGGGGTGCCTCCTGACAGCTTCCAAAGA GCTGACTGTGGAGGGCCCACAGTGTATCCCACCCCTGCCCGAGAGTCTCTCTCCTTTGGG ACCCCCGGGTTCCCAGAGCTCCGAGGAAACTGATCCTTCATGTTTCAATAGCTGTTGTTG TTTTTCTCTTTGGGCCGGGCCGGGCCGGGGAAGGGCCGAGCAGGCTCCAGGAGGCCTCCA GAGTCAGCTGGACCTTGCTGTGTCGCCCGCAGGCAGCCCTGCAGACACCGGTGAGGTGGG ATGATTTCAGGAGGTCCCAGCCCGGGACTGCCCGCCCCTCTGCCCTGGGAACCAGTCCAA GGGGCTGTCAGTTGAATTTGCCTTTTTGTTGTTGTTGTTGTTTTTGTTGTTTATTTGTTC TCCTTTTAGGAATTATGAAGCTAANAAAGTTTTGTGTTTAGAGGGTTAGTANACAAAGTT CTAGCTGATTAATATGGTCTAACTATTGAACTATTTGTTTTCTTTAATGTGNACTGTGGA GAAAAACTATTTGAGCATTGGGGTAGGG
Restriction Sites	Please inquire
ACCN	BC010010
Insert Size	1075 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010010.1, AAH10010.1
RefSeq Size	1075 bp
Locus ID	8178
Protein Families	Transcription Factors
Gene Summary	Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.[UniProtKB/Swiss-Prot Function]

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