SPACA1 (BC029488) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MGC32952; OTTHUMP00000016837; SAMP32; sperm acrosomal membrane-associated protein 32; sperm acrosome associated 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC029488 edited GTCACGGGATTCGGAGCCGGGCGGCTACGGGCGGGGTGTCGCAGCTCTCTTCGACGTACC TGTCCTCAGGAGCCGCGGCGGCGACTGCGCCTCGGACGGCCGTCGGGGCCGAGAACCATG AGCCCCAGGGGCACGGGCTGCTCCGCCGGGCTGCTGATGACTGTCGGCTGGCTGCTTCTG GCGGGCCTCCAGTCCGCGCGCGGGACCAACGTCACCGCTGCCGTCCAGGATGCCGGCCTG GCCCACGAAGGCGAGGGCGAGGAGGAGACCGAAAACAACGACAGCGAGACCGCGGAGAAC TACGCTCCGCCTGAAACCGAGGATGTTTCAAATAGGAATGTCGTCAAAGAAGTAGAATTC GGAATGTGCACCGTTACATGTGGTATTGGTGTTAGAGAAGTTATATTAACAAATGGATGC CCTGGTGGTGAATCCAAGTGTGTTGTACGGGTAGAAGAATGCCGTGGACCAACAGATTGT GGCTGGGGTAAACCAATTTCAGAAAGTCTTGAAAGTGTTAGATTGGCATGTATTCACACA TCTCCCTTAAATCGTTTCAAATATATGTGGAAACTTCTAAGACAAGACCAACAATCCATT ATACTTGTAAATGATTCAGCAATCCTAGAAGTACGCAAGGAAAGTCACCCCTTGGCTTTC GAGTGTGACACACTGGATAATAATGAAATAGTAGCAACTATTAAATTCACAGTCTATACG AGCAGTGAATTGCAGATGAGAAGATCAAGCCTACCAGCCACTGATGCAGCCCTAATTTTT GTGCTGACCATAGGAGTCATTATCTGTGTATTTATAATTTTCTTATTGATCTTCATAATC ATAAATTGGGCAGCAGTCAAGGCTTTCTGGGGGGCAAAAGCCTCTACACCCGAGGTACAA TCCGAGCAGAGTTCTGTGAGATACAAAGATTCAACTTCTCTTGACCAATTACCAACAGAA ATGCCTGGTGAAGATGATGCTTTAAGTGAATGGAATGAATGATGTTTGAATGATATATAA CAAACCAAAGGATATTACAGAATATTAGATTCATTATTACAAAAATAAAATACACATTGA AATACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029488 unedited AGTTTAGATTTGTATACCACTTCTATAGGCGGCCGCATAACTTCGTATAGCATACATTAT ACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGTCACGGG ATTCGGAGCCGGGCGGCTACGGGCGGGGTGTCGCAGCTCTCTTCGACGTACCTGTCCTCA GGAGCCGCGGCGGCGACTGCGCCTCGGACGGCCGTCGGGGCCGAGAACCATGAGCCCCAG GGGCACGGGCTGCTCCGCCGGGCTGCTGATGACTGTCGGCTGGCTGCTTCTGGCGGGCCT CCAGTCCGCGCGCGGGACCAACGTCACCGCTGCCGTCCAGGATGCCGGCCTGGCCCACGA AGGCGAGGGCGAGGAGGAGACCGAAAACAACGACAGCGAGACCGCGGAGAACTACGCTCC GCCTGAAACCGAGGATGTTTCAAATAGGAATGTCGTCAAAGAAGTAGAATTCGGAATGTG CACCGTTACATGTGGTATTGGTGTTAGAGAAGTTATATTAACAAATGGATGCCCTGGTGG TGAATCCAAGTGTGTTGTACGGGTAGAAGAATGCCGTGGACCAACAGATTGTGGCTGGGG TAAACCAATTTCAGAAAGTCTTGAAAGTGTTAGATTGGCATGTATTCACACATCTCCCTT AAATCGTTTCANATATATGTGGAAACTTCTAAGACAAGACCAACAATCCATTATACTTGT AAATGATTCAGCAATCCTAGGAGTACGCAAGGAAAGTCACCCCTTGGCTTTCGAGTGTGA CACACTGGGATAATAATGAAATAGTAGCAACTATTAAATCACAGTCTATACGAGCAGTGA ATTGCAGATGAGAAGATCAAGCCTACAGCCCCTGATGCAGCCCTAAATTTTGTGCTGACC ATAGAGTCATATCTGGGGAT
Restriction Sites	Please inquire
ACCN	BC029488
Insert Size	1115 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029488.1, AAH29488.1
RefSeq Size	1115 bp
RefSeq ORF	882 bp
Locus ID	81833
Protein Families	Transmembrane
Gene Summary	The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.