MED18 (BC002694) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ20045; mediator complex subunit 18; mediator of RNA polymerase II transcription, subunit 18 homolog; OTTHUMP00000003645; p28b; TRAP/mediator complex subunit p28b
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002694 edited CCGATCTGGGGGCTTCCCGGGCTCGGGTAACCGGAGTGCTGGTATCTAATCGTCGCTCAA AAGCTCCTAGGTGCGCGGGTGGATATAATTCCCGGCTTCTCGAGAAGACACTACAGCCTC AAGGAGGTATATCCCGTGCCTTACCTGACTGGGGGCTCTGAGTCCAGTTGTGTTGTCTTC AACTTAGACACCATGGAGGCACCTCCAGTCACCATGATGCCTGTCACTGGGGGCACCATT AACATGATGGAGTACCTGTTGCAGGGAAGTGTTTTAGATCACAGTTTGGAAAGCCTCATC CACCGCCTTCGTGGTTTGTGTGACAACATGGAACCTGAGACTTTCCTTGACCATGAGATG GTATTCCTCCTTAAGGGCCAGCAAGCCAGCCCATTTGTTCTCAGGGCCCGACGCTCTATG GACAGGGCAGGGGCACCCTGGCATCTGCGCTACCTGGGACAGCCAGAAATGGGAGACAAG AACCGCCATGCCCTGGTGCGAAACTGCGTGGACATTGCCACATCTGAGAACCTCACCGAC TTCTTGATGGAAATGGGCTTCCGCATGGACCATGAGTTTGTTGCTAAGGGACATTTGTTC CGTAAGGGCATCATGAAGATTATGGTGTACAAGATTTTCCGCATCCTGGTGCCAGGGAAC ACAGACAGCACTGAGGCCTTGTCACTCTCCTATCTCGTGGAATTAAGTGTGGTAGCACCC GCTGGGCAGGACATGGTCTCTGATGACATGAAGAACTTCGCAGAACAGCTAAAACCTCTG GTTCACCTAGAGAAAATAGACCCCAAGAGGCTCATGTGACTAAGAGGATCTGTCCACATT TGGGGCCTATCCTTACTTGTTTGAAAAAATATGTTTGCTTTTTTTGGTTTTTGTTTTGTT TTGTTTTTGAGACGGAGTCTCGCTTTGCACTCCAGCCTGGGTGAGAAGAGCGAAACTCCG TCTCAAAAAAAAATAAAAATGAAGAGGGGAAACCAGAATAAATTATCTTTTGGAAAGGAC AATTTCTTGTTTGGCCATTTGTGTATAAGGTTGGTAACATTAGAGGCTGTGAGCTTGTGT TACATGGTAATAAAGCCAATGAAGAGACAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002694 unedited GGACATACTCGGTAATTTGTAAATACGACCCACTATTAGTGGCGGCCGCGCAATTCGGCA CGAGGCCGATCTGGGGGCTTCCCGGGCTCGGGTAACCGGAGTGCTGGTATCTAATCGTCG CTCAAAAGCTCCTAGGTGCGCGGGTGGATATAATTCCCGGCTTCTCGAGAAGACACTACA GCCTCAAGGAGGTATATCCCGTGCCTTACCTGACTGGGGGCTCTGAGTCCAGTTGTGTTG TCTTCAACTTAGACACCATGGAGGCACCTCCAGTCACCATGATGCCTGTCACTGGGGGCA CCATTAACATGATGGAGTACCTGTTGCAGGGAAGTGTTTTAGATCACAGTTTGGAAAGCC TCATCCACCGCCTTCGTGGTTTGTGTGACAACATGGAACCTGAGACTTTCCTTGACCATG AGATGGTATTCCTCCTTAAGGGCCAGCAAGCCAGCCCATTTGTTCTCAGGGCCCGACGCT CTATGGACAGGGCAGGGGCACCCTGGCATCTGCGCTACCTGGGACAGCCAGAAATGGGAG ACAAGAACCGCCATGCCCTGGTGCGAAACTGCGTGGACATTGCCACATCTGAGAACCTCA CCGACTTCTTGATGGAAATGGGCTTCCGCATGGACCATGAGTTTGTTGCTAAGGGACATT TGTTCCGTAAGGGCATCATGAAGATTATGGTGTACAAGATTTTCCGCATCCTGGTGCCAG GGAACACAGACAGCACTGAGGCCTTGTCACTCTCCTATCTCGTGGAATTAAGTGTGGTAG CCCCCGCTGGGCANGACATGGTCTCTGATGACATGAAGAACTTCGCAGAACAGCTAAAAC CTCTGGNTCACCTANAGAAAATAGACCCCAGGAGCTCATGTGACTAAGAAGATCTGTCCA CATTGGGGCCTATTCT
Restriction Sites	Please inquire
ACCN	BC002694
Insert Size	1135 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002694.2, AAH02694.1
RefSeq Size	1126 bp
Locus ID	54797
Gene Summary	MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.