TEF (BC021980) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC021980 edited GGCACGAGGATTTTGTTATTAGTGGAAGAGCCTTGAGCTTAAAAGCTCTTCATTTTCTTT GCCGGAAAGTATATGGTTTGTGTTTTTGCAGTTTTATTTTTTTAAGGATGGACACTAAAT CTCTGGTGTCCATGTTCCGAGCCCGGTGGCTGGGATGGTGGCTCGATGTGTCTCCTCTTT ATCCCGCCCTCTGCCTGTTTGGTGCCCTCCCTTCTTTCCCCCAGGCAGTGGGTATCGGGT TCTTTCCCAAAGTGCTTACTTGGAAAGAGTGTGGCTGCTGGACTCTTTTCGAAATGCCCC TCTGAGTCAGGAGCCTGGTGGGGACAAGATGGAGGATGTCCACAGAGGCTGACCTGTGGG GGAAAAGAAGTGCTCAGGTAAGGTTGTCTCCTCTCCTGTCCTAAAAAAATCCATGCTTGC AGGAGAGGGTTGGTGCCTGCTCAGTTTCTGTCGAGGGAATGGGAGTCTCCTCGGCTCCCT CCTGGGCCCTCTGCTTCTTGGAGGCCGACCGCTGGAGACTGCGGCTCCTGTGCTTGGATC TTTGACATCTGTCAGTCACTGGAGGCTAGAGAAGTTGACTTTACCTTGGCTTTCTGTGGG TTCCCGATGGGCTTGGCAAGTGCCGTCTACTTCCTAAGCATCCTGTCTAAAGCTTTGTGG CACCCTCAGTGCAACCTCAGAACAGACAAATCATGAATGAGCTGGAACTTTGCAAGAATA TTCATATTATAAATGTCTCATCTGATGGAGGAACGTGTGCATTTAGAGAGAGGGAGAGTT TTCAAGGTTTATGGCAACTTTGTGGAGGACCTGGGTAACTCTTACCCTTGGCCAAAGGAA GAGGTTTTCCTGTCTGGCTTCTGAGGTTGGAGGGGGCACTTAGCAGTGAACCTTAAGTCT GGGTACATGTAACCCTGCTGAGGGGCTGTGCAGGCCGCTCCTACGGTCCTTTGGCCTGAG GCAGGGCGGGAGGCATGCAAGCCAGTGGGGGAAAACCCCTCTGAAGCTGGGCAGCCCTCT ACCTGGGCCCCGGGAGCCGTGCTCCTTGGAACGCAAAGGGCCGAGGAGCATCTGGTTTTC ATGGCAAAGCTCTACTCCAGAGCTCCTTTAACATCTGCTAATTAAGTGCAATAAATTTTT CTAGAAAATGGCAAAGATGACTTCCAGGTGGATATTGCTCTCTTACGGTGTTGGGGATGC CAGAACACCACTTGGTTTTATTTTTCTAAGTGCATGTGATGTGATAGAGTGTGTGGGGCT CTGTGTCCTTCCCTGGGAGCTGGCATTCCAGCGGGCCCCTCTCTTTACCTTTGTTGGGGG AAGGAGGCAAGAGAGAAATTCCTTCTTCCCAGCCAGAGAGGGCAGAAGCAGACCGTAGCC CATTGGCCTTATGTGCGTGTGTGCGTGCGAGTGTGTCACTGCTGGTGGGCCGGAGTGATG TGGTGGGAGGGAAGCCGGGAATGTATCCTTTTCAGACAAAATTAAATATTTTGAAATGAG AAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021980 unedited NCTTTCAGATTTGTATCACGACTTATATAGGCGGCCGCGCATTCGGCACGAGGATTTTGT TATTAGTGGAAGAGCCTTGAGCTTAAAAGCTCTTCATTTTCTTTGCCGGAAAGTATATGG TTTGTGTTTTTGCAGTTTTATTTTTTTAAGGATGGACACTAAATCTCTGGTGTCCATGTT CCGAGCCCGGTGGCTGGGATGGTGGCTCGATGTGTCTCCTCTTTATCCCGCCCTCTGCCT GTTTGGTGCCCTCCCTTCTTTCCCCCAGGCAGTGGGTATCGGGTTCTTTCCCAAAGTGCT TACTTGGAAAGAGTGTGGCTGCTGGACTCTTTTCGAAATGCCCCTCTGAGTCAGGAGCCT GGTGGGGACAAGATGGAGGATGTCCACAGAGGCTGACCTGTGGGGGAAAAGAAGTGCTCA GGTAAGGTTGTCTCCTCTCCTGTCCTAAAAAAATCCATGCTTGCAGGAGAGGGTTGGTGC CTGCTCAGTTTCTGTCGAGGGAATGGGAGTCTCCTCGGCTCCCTCCTGAGCCCTCTGCTT CTTGGAGGCCGACCGCTGGAGACTGCGGCTCCTGTGCTTGGATCTTTGACATCTGTCAGT CACTGGAGGCTAGAGAAGTTGACTTTACCTTGGCTTTCTGTGGGTTCCCGATGGGCTTGG AAGTGCCGTCTACTTCCTAAGCATCCTGTCTAAAGCTTTGTGGCACCCTCAGTGCANCCT CAGAACAGACAAATCATGAATGAGCTGGNACTTTTGCAGATATTCATATATAATGTCTAT CTGATGAGGACGTGTGCTTTAAAAGAGGAAAGTTCAAGTTATGCACTTGGAACGGACTTA CTGCAGAAGGTCGTTGTAGTGGGCTACGACTACGACTACGGAGGTGTGCCTGA
Restriction Sites	Please inquire
ACCN	BC021980
Insert Size	1518 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021980.1, AAH21980.1
RefSeq Size	1518 bp
Locus ID	7008
Protein Families	Transcription Factors
Gene Summary	This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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