Collagen IX (COL9A1) (BC015409) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	alpha-1(IX) collagen chain; alpha 1 type IX collagen; cartilage-specific short collagen; collagen, type IX, alpha 1; collagen IX, alpha-1 polypeptide; DJ149L1.1.2; EDM6; FLJ40263; MED
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015409 edited CCCTCACCGGGGGCAGGAGGGACCAAGGCTGGGCCCAGAACACATAGTCCTAGGGTAACA GTGAAGGGGTCGTGAGGGGACAGTGACTCCCTTCCAACCCCTTCTTCATAGGGACTGTTG GCAAACAAAGAAAATCAACTGGGAAAATGAAGACCTGCTGGAAAATTCCAGTTTTCTTCT TTGTGTGCAGTTTCCTGGAACCCTGGGCATCTGCAGCTGTCAAGCGTCGCCCCAGATTCC CTGTCAATTCCAATTCTAATGGTGGAAATGAACTCTGTCCAAAGATCAGGATTGGCCAAG ATGACTTACCAGGGTTTGATCTGATCTCTCAGTTCCAGGTAGATAAAGCAGCATCTAGAA GAGCTATCCAGAGAGTAGTGGGATCAGCTACATTGCAGGTGGCTTACAAGTTGGGAAATA ATGTAGACTTCAGGATTCCAACTAGGAATTTATATCCCAGTGGACTGCCTGAAGAATACT CCTTCTTGACGACGTTTCGAATGACTGGAAGCACTCTCAAAAAGAACTGGAACATTTGGC AGATTCAGGATTCCTCTGGGAAGGAGCAAGTTGGCATAAAGATTAATGGCCAAACACAAT CTGTTGTATTTTCATACAAGGGACTGGATGGAAGTCTCCAAACAGCAGCCTTTTCGAATT TGTCCTCCTTGTTTGATTCCCAGTGGCATAAGATCATGATTGGCGTGGAGAGGAGTAGTG CTACTCTTTTTGTTGACTGCAACAGGATTGAATCTTTACCTATAAAGCCAAGAGGCCCAA TTGACATTGATGGCTTTGCTGTGCTGGGAAAACTTGCAGATAATCCTCAAGTTTCTGTTC CATTTGAACTTCAATGGATGCTGATCCATTGTGACCCCCTGCGGCCCAGGAGAGAAACTT GCCATGAGCTGCCAGCCAGAATAACGCCCAGCCAGACCACCGACGAGAGAGGTCCCCCGG GTGAGCAGGGTCCTCCCGGGCCTCCGGGCCCCCCTGGAGTTCCAGGCATCGATGGCATCG ACGGTGACCGAGGTCCTAAGGGCCCCCCGGGCCCCCCGGGTCCTGCAGGTGAACCGGGAA AGCCAGGAGCTCCAGGCAAGCCTGGCACACCTGGCGCTGATACCAGTCCTTGACTTTGTC CCTGGAATATGAAGTGAAGTAAGAAATGGCGTCTGTTCTTAGCATACCATGGTGCATCAC TCAAGTTACTCTTCAAGTTACCACTTTATGTGTTTTCCAAAAACTAAAGACTTTTCTAAA TGAAAGAAAGCACTCGAAAACCTATAATTATGATTTCCAAGAATTTTTGACTACAGTTTA AGACATATCCTTCATTTTGAAAGAAAAATAAATAACTGGATTTTTACTGTTAAAAAAAAA AAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015409 unedited CCCCGATTAGGATTAGNANAACCACTTTATATAGGCGGCACGCGATTCCCGGGATATCGT CGACCCACGCGTCCGCCCTCACCGGGGGCAGGAGGGACCAAGGCTGGGCCCAGAACACAT AGTCCTAGGGTAACAGTGAAGGGGTCGTGAGGGGACAGTGACTCCCTTCCAACCCCTTCT TCATAGGGACTGTTGGCAAACAAAGAAAATCAACTGGGAAAATGAAGACCTGCTGGAAAA TTCCAGTTTTCTTCTTTGTGTGCAGTTTCCTGGAACCCTGGGCATCTGCAGCTGTCAAGC GTCGCCCCAGATTCCCTGTCAATTCCAATTCTAATGGTGGAAATGAACTCTGTCCAAAGA TCAGGATTGGCCAAGATGACTTACCAGGGTTTGATCTGATCTCTCAGTTCCAGGTAGATA AAGCAGCATCTAGAAGAGCTATCCAGAGAGTAGTGGGATCAGCTACATTGCAGGTGGCTT ACAAGTTGGGAAATAATGTAGACTTCAGGATTCCAACTAGGAATTTATATCCCAGTGGAC TGCCTGAAGAATACTCCTTCTTGACGACGTTTCGAATGACTGGAAGCACTCTCAAAAAGA ACTGGAACATTTGGCAGATTCAGGATTCCTCTGGGAAGGAGCAAGTTGGCATAAAGATTA ATGGCCAAACACAATCTGTTGTATTTTCATACAAGGGACTGGATGGAAGTCTCCAAACAG CAGCCTTTTCGAATTTGTCCTCCTTGTTTGATTCCCAGTGGCATAAGATCATGATTGGCG TGGAGAGGAGTAGTGCTACTCTTTTTGTTGACTGCAACAGGATTGAATCTTTACCTATNA AGCCAAGAGGCCCAATTGACATTGATGGGCTTGCTGTGCTGGGAAAACTTGCAGANATCC TCAA
Restriction Sites	Please inquire
ACCN	BC015409
Insert Size	1387 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015409.1, AAH15409.1
RefSeq Size	1387 bp
Locus ID	1297
Protein Families	Druggable Genome
Gene Summary	This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

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