DENND5A (NM_015213) Human Untagged Clone
CAT＃: SC121400
DENND5A (untagged)-Human DENN/MADD domain containing 5A (DENND5A)
CNY 8968.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE49; EIEE49; RAB6IP1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC121400 sequence for NM_015213 edited (data generated by NextGen Sequencing)
ATGAGTGGCGGCGGCGGCGGAGGGGGCTCGGCGCCCAGTCGCTTCGCCGACTACTTTGTC ATCTGCGGACTGGACACGGAGACCGGGCTGGAGCCGGACGAGCTGTCGGCATTATGCCAG TACATACAGGCTTCTAAAGCCAGGGATGGTGCCAGCCCTTTCATTTCAAGTACGACTGAA GGAGAAAATTTTGAGCAGACACCATTGAGAAGAACATTCAAATCTAAGGTCCTTGCACGA TATCCTGAGAACGTAGAATGGAATCCCTTTGACCAAGATGCAGTAGGAATGCTATGTATG CCGAAAGGGCTGGCATTCAAGACCCAGGCTGATCCCAGGGAGCCCCAATTCCATGCCTTT ATTATCACAAGGGAGGATGGCTCTCGGACATTTGGGTTTGCCCTCACATTTTATGAAGAG GTGACTAGCAAGCAGATCTGCAGTGCAATGCAGACCCTCTACCACATGCACAATGCTGAG TATGATGTCCTACATGCTCCCCCTGCTGATGACAGAGACCAGAGCAGCATGGAGGATGGT GAAGACACTCCTGTGACCAAACTGCAGCGCTTCAACTCCTATGACATTAGCCGGGACACT CTCTACGTCTCTAAGTGCATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGG AGCGTGCTGCAGCAACTCCACCAGGCAGTCACTTCACCTCAGCCCCCTCCACTGCCCCTT GAGAGCTACATATACAACGTACTCTACGAGGTGCCGCTCCCACCTCCTGGCCGGTCCTTG AAGTTTTCTGGGGTCTATGGGCCAATAATCTGCCAGAGACCAAGTACCAATGAGCTTCCC CTATTTGACTTTCCTGTCAAAGAGGTTTTTGAACTGCTCGGGGTGGAGAATGTGTTTCAG CTTTTTACTTGTGCCCTTCTGGAGTTTCAAATCCTGCTCTACTCACAGCATTACCAGAGA CTGATGACTGTGGCGGAGACGATTACAGCTCTCATGTTTCCTTTCCAGTGGCAGCATGTC TATGTCCCTATTCTCCCAGCTTCTCTCCTGCATTTCTTAGATGCTCCTGTTCCATACCTG ATGGGTTTGCATTCCAATGGCCTGGATGACCGGTCAAAGCTGGAGCTGCCTCAAGAGGCT AACCTCTGCTTTGTGGACATTGACAACCACTTCATTGAGTTGCCAGAGGACTTGCCACAG TTCCCCAACAAATTGGAGTTTGTCCAGGAAGTCTCTGAGATTCTCATGGCATTTGGAATT CCCCCTGAAGGGAATCTTCATTGCAGTGAGAGTGCCTCCAAGCTGAAGAGGCTGCGGGCC TCTGAGCTTGTCTCGGACAAGAGGAATGGGAACATTGCTGGCTCCCCTTTGCATTCCTAC GAGCTTCTTAAGGAGAATGAAACTATTGCCCGGCTGCAAGCCTTGGTCAAGAGAACTGGG GTGAGCCTGGAAAAGTTGGAAGTGCGTGAAGACCCCAGCAGCAATAAGGATCTCAAAGTT CAGTGTGATGAAGAAGAACTCAGGATTTACCAGCTAAACATTCAGATCCGGGAAGTTTTT GCAAATCGTTTCACTCAGATGTTTGCAGATTATGAGGTGTTTGTCATCCAACCCAGCCAG GATAAGGAATCCTGGTTTACCAACAGGGAGCAAATGCAAAACTTTGATAAAGCATCTTTT CTGTCAGATCAGCCTGAGCCCTACCTGCCCTTCCTCTCAAGATTCCTGGAGACCCAGATG TTTGCATCTTTCATTGACAACAAAATAATGTGTCATGATGATGATGATAAAGACCCTGTA CTCCGGGTATTTGATTCCCGAGTTGACAAGATCAGGCTGTTGAATGTTCGGACACCTACT CTCCGTACATCCATGTACCAGAAGTGTACCACTGTGGATGAAGCAGAGAAAGCAATTGAG CTGCGTCTGGCAAAAATTGACCATACTGCAATTCACCCACATTTACTTGACATGAAGATT GGACAAGGGAAATATGAGCCGGGCTTCTTCCCTAAGCTGCAGTCTGATGTACTTTCCACT GGGCCAGCCAGCAACAAGTGGACGAAAAGGAATGCCCCTGCCCAGTGGAGGCGGAAAGAT CGGCAGAAGCAGCACACAGAACACCTGCGTTTAGATAATGACCAGAGGGAGAAGTACATC CAGGAAGCCAGGACTATGGGCAGCACTATCCGCCAGCCCAAACTGTCCAACCTCTCTCCA TCAGTGATTGCCCAGACCAATTGGAAGTTTGTAGAGGGCCTGCTGAAGGAATGCCGCAAT AAGACCAAGAGGATGCTGGTGGAAAAGATGGGCCGAGAAGCTGTGGAGCTAGGGCATGGG GAGGTGAACATCACAGGGGTGGAAGAGAACACCCTGATTGCCAGCCTTTGTGATCTCCTG GAAAGGATCTGGAGTCATGGACTACAAGTGAAACAGGGGAAATCAGCCTTATGGTCCCAC CTGTTACATTATCAGGACAACCGGCAGAGAAAACTCACATCAGGAAGCCTCAGTACCTCA GGAATACTTCTTGATTCAGAACGTAGGAAGTCTGATGCCAGCTCACTCATGCCTCCCCTG AGGATCTCCCTGATTCAGGATATGAGGCACATCCAGAACATCGGGGAAATCAAGACTGAT GTGGGAAAGGCCAGAGCATGGGTGCGACTGTCCATGGAAAAAAAGTTACTTTCCAGACAC CTGAAGCAGCTCCTCTCAGACCATGAGCTCACCAAAAAGTTATATAAGCGCTATGCCTTC CTGCGCTGTGATGACGAGAAGGAGCAGTTCCTCTATCACCTCCTGTCTTTCAATGCCGTC GATTACTTTTGCTTCACCAATGTCTTCACAACTATCCTGATCCCGTACCACATTCTGATC GTACCAAGCAAGAAGCTGGGGGGCTCCATGTTCACTGCCAACCCATGGATCTGTATATCA GGAGAATTGGGTGAGACACAGATCATGCAGATTCCCAGGAATGTGCTAGAGATGACCTTC GAGTGCCAGAACTTGGGGAAGCTTACTACTGTCCAGATTGGCCATGATAACTCTGGGCTG TATGCCAAATGGCTGGTGGAGTATGTGATGGTCAGGAATGAGATCACAGGACATACCTAC AAGTTCCCGTGTGGCCGGTGGTTAGGGAAGGGCATGGATGATGGAAGCCTGGAGCGGATC CTAGTTGGGGAGCTGCTCACATCCCAGCCTGAGGTGGATGAGAGGCCATGCCGGACCCCG CCGCTGCAGCAGTCCCCCAGTGTCATCCGGAGGCTTGTTACCATCTCACCCAACAACAAG CCCAAGCTGAACACTGGGCAGATCCAGGAGTCCATCGGGGAGGCAGTCAATGGCATTGTG AAGCACTTCCATAAGCCTGAGAAAGAGCGAGGCAGTCTGACGCTGTTGCTCTGTGGAGAG TGTGGCCTTGTCTCGGCCTTGGAACAGGCTTTCCAGCATGGATTTAAATCGCCCCGGCTC TTCAAAAATGTCTTCATTTGGGATTTCCTGGAAAAAGCACAAACCTATTATGAGACATTA GAGAAGAATGAAGTAGTCCCTGAGGAAAACTGGCATACAAGAGCCCGGAACTTCTGCCGA TTTGTCACTGCAATCAACAATACTCCCCGGAACATCGGCAAGGATGGCAAGTTTCAGATG CTGGTGTGCTTGGGAGCCAGAGATCACCTCCTACACCACTGGATTGCCCTGCTGGCTGAC TGCCCCATCACTGCACACATGTATGAGGATGTGGCACTGATCAAAGACCATACACTTGTC AATTCCTTGATTCGTGTGCTGCAGACATTGCAGGAGTTCAACATCACGCTGGAGACGTCC CTTGTCAAGGGCATCGACATCTGA Clone variation with respect to NM_015213.3 670 g=>c >OriGene 5' read for NM_015213 unedited
CCGCGAATTCGGCACGAGGGAGGGGCGGCCTGAGGGCGGACGGGCGGGCGCCCGGGTTGC GGGGGCTCGGTGCCGCTCCGCACTGCCCGGCCGGTCTCGGCCCCGGCGCCATGAGTGGCG GCGGCGGCGGAGGGGGCTCGGCGCCCAGTCGCTTCGCCGACTACTTTGTCATCTGCGGAC TGGACACGGAGACCGGGCTGGAGCCGGACGAGCTGTCGGCATTATGCCAGTACATACAGG CTTCTAAAGCCAGGGATGGTGCCAGCCCTTTCATTTCAAGTACGACTGAAGGAGAAAATT TTGAGCAGACACCATTGAGAAGAACATTCAAATCTAAGGTCCTTGCACGATATCCTGAGA ACGTAGAATGGAATCCCTTTGACCAAGATGCAGTAGGAATGCTATGTATGCCGAAAGGGC TGGCATTCAAGACCCAGGCTGATCCCAGGGAGCCCCAATTCCATGCCTTTATTATCACAA GGGAGGATGGCTCTCGGACATTTGGGTTTGCCCTCACATTTTATGAAGAGGTGACTAGCA AGCAGATCTGCAGTGCAATGCAGACCCTCTACCACATGCACAATGCTGAGTATGATGTCC TACATGCTCCCCCTGCTGATGACAGAGACCAGAGCAGCATGGAGGATGGTGAAGACACTC CTGTGACCAAACTGCAGCGCTTCAACTCCTATGACATTAGCCGGGACACTCTCTACGTCT T |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015213 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015213.2, NP_056028.2 |
| RefSeq Size | 4965 bp |
| RefSeq ORF | 3864 bp |
| Locus ID | 23258 |
| UniProt ID | Q6IQ26 |
| Gene Summary | This gene encodes a DENN-domain-containing protein that functions as a RAB-activating guanine nucleotide exchange factor (GEF). This protein catalyzes the conversion of GDP to GTP and thereby converts inactive GDP-bound Rab proteins into their active GTP-bound form. The encoded protein is recruited by RAB6 onto Golgi membranes and is therefore referred to as RAB6-interacting protein 1. This protein binds with RAB39 as well. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene are associated with early infantile epileptic encephalopathy-49. [provided by RefSeq, Feb 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209850 | DENND5A (Myc-DDK-tagged)-Human DENN/MADD domain containing 5A (DENND5A) |
CNY 8960.00 |
|
| RC209850L1 | Lenti ORF clone of Human DENN/MADD domain containing 5A (DENND5A), Myc-DDK-tagged |
CNY 11360.00 |
|
| RC209850L2 | Lenti ORF clone of Human DENN/MADD domain containing 5A (DENND5A), mGFP tagged |
CNY 14730.00 |
|
| RC209850L3 | Lenti ORF clone of Human DENN/MADD domain containing 5A (DENND5A), Myc-DDK-tagged |
CNY 14730.00 |
|
| RC209850L4 | Lenti ORF clone of Human DENN/MADD domain containing 5A (DENND5A), mGFP tagged |
CNY 14730.00 |
|
| RG209850 | DENND5A (tGFP-tagged) - Human DENN/MADD domain containing 5A (DENND5A) |
CNY 14160.00 |
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