CCDC52 (SPICE1) (NM_144718) Human Untagged Clone
CAT＃: SC120766
SPICE1 (untagged)-Human spindle and centriole associated protein 1 (SPICE1)
CNY 9400.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCDC52; SPICE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120766 sequence for NM_144718 edited (data generated by NextGen Sequencing)
ATGTCATTTGTCAGAGTGAACCGCTGTGGTCCCCGAGTTGGTGTAAGAAAGACACCGAAA GTAAAGAAGAAGAAAACTTCAGTGAAACAAGAATGGGATAATACCGTGACTGATCTAACC GTTCATCGGGCAACTCCTGAAGATCTGGTACGCCGTCATGAAATACACAAATCGAAGAAT AGAGCATTAGTACACTGGGAACTCCAAGAAAAAGCTTTGAAGAGAAAATGGAGGAAGCAG AAACCAGAAACTTTAAATCTTGAGAAAAGAAGATTGTCTATCATGAAGGAGATTCTTTCT GATCAATACCAGATGCAAGATGTGTTGGAGAAATCTGATCATCTAATAGCTGCAGCAAAA GAGCTGTTTCCTCGTAGGCGCACAGGGTTTCCAAATGTAACAGTGGCTCCTGATTCCTCT CAGGGTCCCATTGTGGTAAATCAAGACCCTATCACCCAATCTATCTTTAATGAGTCTGTC ATAGAACCTCAGGCTCTTAATGATGTAGATGGTGAAGAAGAAGGAACTGTTAATAGCCAG TCAGGAGAAAGTGAGAATGAGAATGAGTTGGATAACTCTCTAAACTCTCAGTCTAACACG AATACAGACAGGTTTCTCCAACAACTAACAGAAGAGAATTTTGAGTTAATTAGTAAGTTG TGGACTGACATTCAGCAGAAAATAGCAACCCAGTCACAAATAACTCCTCCAGGAACGCCA TCATCTGCTCTTTCATCAGGGGAGCAAAGAGCTGCTCTGAATGCTACCAATGCTGTCAAG AGACTCCAAACCAGGCTTCAGCCTGAAGAATCTACTGAGACTCTAGACTCAAGCTACGTT GTGGGACACGTGCTGAACTCAAGGAAGCAAAAACAGCTGTTAAATAAAGTGAAAAGGAAA CCGAATTTGCATGCTCTTTCCAAGCCGAAGAAAAACATATCATCAGGTAGCACAACCTCT GCAGACTTACCAAATAGGACTAATTCCAACCTGGATGTCCTCAAACACATGATACATGAA GTGGAACATGAAATGGAAGAATATGAGCGGTGGACAGGTCGCGAGGTCAAGGGTCTGCAG AGCAGTCAGGGTCTTACAGGCTTCACTTTGTCGCTGGTGAGCTCCCTCTGTCGCCTGGTT CGGTACCTTAAAGAGAGTGAGATCCAGCTACGTAAAGAAGTAGAGACAAGGCAACAACTG GAACAAGTATTAGGTGATCATCGAGAGCTCATTGATGCTCTGACAGCTGAAATTCTTCGT CTTAGAGAAGAAAACGCTGCTACACAKGCAAGACTTCAGCAGTACATGGTCACAACAGAT GAGCAACTGATATCACTCACACATGCTATTAAGAACTGTCCTGTGATAAATAACAGACAA GAAATTCAGGCATCAGAAAGCGGAGCCACAGGTAGAAGAGTTATGGACAGTCCAGAGCGT CCAGTTGTAAATGCCAATGTCTCAGTGCCATTGATGTTCAGAGAGGAAGTGGCTGAATTC CCACAGGAAGAGTTGCCTGTTAAACTGTCTCAGGTGCCAGACCCTCCAGATAACATGAAT CTGGCCAAGAATTTTCCAGCACATATTTTTGAGCCAGCTGTGTTGTTAACACCACCCAGG CAGAAGAGCAACTTAAAATTCTCTCCTCTTCAGGACGTATTGAGAAGGACTGTTCAAACT CGTCCTGCTCCACGACTTCCTCCAACTGTGGAAATAATTGAGAAGGAACAAAATTGGGAA GAGAAGACCTTACCTATTGATACAGACATTCAGAATTCAAGTGAAGAGAATCGTCTCTTC ACTCAGAGATGGAGAGTCTCTCACATGGGAGAAGATTTGGAGAACAAAACTCAGGCTCCT TTTGTTAACCTCTCACAGCCCCTCTGCAATTCCCATTCCAACACTCAACAGTCAAGAAGC CCCACATTCTCAGAAGAGCTCCCAGTACTGGGAGATGGGCAGCAGCTGAGAACAAATGAG TCATTAATACAAAGAAAGGACATAATGACACGAATTGCTGATTTGACATTGCAGAATTCA GCTATCAAGGCACATATGAATAATATTATTGAGCCCAGAGGAGAGCAAGGGGATGGACTC CGGGAGTTGAACAAACAAGAAAGTGCAAGTGACATGACTTCTACTTTTCCAGTAGCACAG TCTCTAACACCAGGTAGTATGGAGGAACGGATTGCAGAATTGAATCGACAAAGTATGGAG GCTCGTGGAAAACTACTGCAGTTGATAGAGCAGCAGAAACTTGTTGGTTTGAATCTTTCT CCACCAATGTCACCTGTTCAGTTACCTCTCAGAGCATGGACTGAAGGAGCAAAGAGGACA ATTGAGGTATCTATTCCAGGAGCAGAAGCCCCAGAAAGCTCAAAATGTAGTACTGTCTCT CCCGTCAGCGGGATAAATACAAGAAGATCTTCCGGGGCTACTGGTAATTCTTGTTCTCCA CTAAATGCCACCTCAGGAAGTGGGAGATTCACACCTCTTAATCCAAGAGCAAAGATTGAG AAACAGAATGAAGAAGGCTGGTTTGCTCTTTCTACCCATGTATCATAA Clone variation with respect to NM_144718.3 138 c=>t;1287 g=>k;1518 c=>t >OriGene 5' read for NM_144718 unedited
TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGCACA GAGCTGATGTCATTTGTCAGAGTGAACCGCTGTGGTCCCCGAGTTGGTGTAAGAAAGACA CCGAAAGTAAAGAAGAAGAAAACTTCAGTGAAACAAGAATGGGATAATACCGTGACTGAT CTAACCGTTCATCGGGCAACTCCTGAAGATCTGGTACGCCGTCATGAAATACACAAATCG AAGAATAGAGCATTAGTACACTGGGAACTCCAAGAAAAAGCTTTGAAGAGAAAATGGAGG AAGCAGAAACCAGAAACTTTAAATCTTGAGAAAAGAAGATTGTCTATCATGAAGGAGATT CTTTCTGATCAATACCAGATGCAAGATGTGTTGGAGAAATCTGATCATCTAATAGCTGCA GCAAAAGAGCTGTTTCCTCGTAGGCGCACAGGGTTTCCAAATGTAACAGTGGCTCCTGAT TCCTCTCAGGGTCCCATTGTGGTAAATCAAGACCCTATCACCCAATCTATCTTTAATGAG TCTGTCATAGAACCTCAGGCTCTTAATGATGTAGATGGTGAAGAAGAAGGAACTGTTAAT AGCCAGTCAGGAGAAAGTGAGAATGAGAATGAGTTGGATAACTCTCTAAACTCTCAGTCT AACACGAATACAGACAGGTTTCTCCAACAACTAACAGAAGAGAATNNTGAGTTAATTAGT AAGTTGTGGACTGACATTCAGCAGAAAATAGCAACCCAGTCACAAATAACTNCTNCAGGA ACGCCATCATCTGCTCTTTCATCAGGNGAGCAAAGAGCTGCTCTGAATGCTACCAATGCT GTCAAGAGACTNCAAACNCAGCTTCAGNCTGAAGAATCTACTGAGACTCTAGACTCAAGC TACNGTGTGGGACACGTGCTNGACTCAGGNAGCANAACAGCTGNTAANTNAAGTGAAAGG AANNCCGATTGCATGCTC >OriGene 3' read for NM_144718 unedited
ATATGTACGGGCCGCTTTCTAGATCGAGTTTTTTTTTTTTTTTTTTGTTTTTAAGGCTGT ATATTTCACTTGATGGAAAAATGATGAACAGCAATGACTTCCCAAGAAAAAAGAAAGAAA AGTGGGTGTTACATATTCCTGAAAGCCAGACTCTAATCACACAGAGGAATTTTATTGTAA GTTACATGCAAAGCAAATTAGTTCTTCAGGAAAATAAATAAATAAATGGATCAACTAAGA TATACTAAACCTCAAAAAACCCTGAATAAAGATAAAGGTAAAAATGTATTAATAAAAAAA ACCCTTAAAATACTTAAGAAAGTATTAAAATACAAAACTTTAACTTCTCTACAGTCAAAG TTTCATTCACAGGGAGCTGTAGGTCAGTTGGTTGTTGAAAACTTATTTGAGAAAGTCAAA AAATAATTGCTTTATTTCTCTGTTTCATTAGTACTAATTCACAGGATCTTTGTAGGTTTT ATCTGAAAGAAGGATATAAAAACTTAAAAGTCAGAGCAGGGAAAGGGGAGTAATAAATTA TTAAGAACAAACTCAGTGAGACTTGACTTCACTTATGATACATGGGTAGAAAGAGCAAAC CAGCCTTCTTCATTCTGTTTCTCAATCTTTGCTCTTGGATTAAGAGGTGTGAATCTCCCA CTTCCTNGAGTGGCATTTAGTGGAGAACAAGAATTACCAGTAGCCCCGGAAGATCTTCTT GTATTTATCCCGCTGACCGGAGAGACAGTACTACATTTTGAGCTTTCTGGGGCCTCTGCT CCTGGATAGATACCTAATTGTNCTCTTTGCTCCTTCAGTCATGCTCTGAGAGGAACTGAA CAGTGACATGGTGGAAAAAGATTCAACAACAGTTTTGCTGTCTATCACTGCGTAGGTTTC CCGAGCCTCCTACTTGGCGATTCATCTGCAAACGTCCTCCTACTCCTGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_144718 |
| Insert Size | 3500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144718.2, NP_653319.1 |
| RefSeq Size | 5445 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 152185 |
| UniProt ID | Q8N0Z3 |
| Gene Summary | Regulator required for centriole duplication, for proper bipolar spindle formation and chromosome congression in mitosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the shorter isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207856 | SPICE1 (Myc-DDK-tagged)-Human spindle and centriole associated protein 1 (SPICE1) |
CNY 9392.00 |
|
| RC207856L3 | Lenti ORF clone of Human spindle and centriole associated protein 1 (SPICE1), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC207856L4 | Lenti ORF clone of Human spindle and centriole associated protein 1 (SPICE1), mGFP tagged |
CNY 9030.00 |
|
| RG207856 | SPICE1 (tGFP-tagged) - Human spindle and centriole associated protein 1 (SPICE1) |
CNY 10992.00 |
