PUS10 (NM_144709) Human Untagged Clone
CAT＃: SC120726
PUS10 (untagged)-Human pseudouridylate synthase 10 (PUS10)
CNY 3944.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCDC139; DOBI; Hup10 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120726 sequence for NM_144709 edited (data generated by NextGen Sequencing)
ATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCAGTTGTTGCTCAATACTGGTACT TGTCCAAGATGTATCTTCAGATTCTGTGGTGTGGATTTTCATGCACCTTACAAACTTCCA TACAAGGAGTTGCTCAATGAACTACAGAAATTTCTGGAAACTGAAAAAGATGAATTAATT TTGGAAGTTATGAACCCACCTCCCAAGAAAATTCGACTGCAAGAACTGGAAGATAGTATT GATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGTTAGTCATGTTGGAAGCACTGCT TCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCTAGGAATTCTTCAAGAATTCTGT GAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGAGGCCTCTGGGTTTGAATTCACC AGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATCTGTAAGAGAGCATGCTGCATGG TTGCTGGTAAAACAGGAAATGGGAAAGCAGAGTCTGTCGCTGGGAAGAGATGATATAGTT CAGCTAAAAGAAGCCTACAAATGGATAACTCACCCCCTGTTTTCAGAGGAACTGGGTGTT CCCATTGATGGAAAGAGCTTGTTTGAAGTGAGTGTGGTCTTTGCTCACCCAGAAACAGTT GAGGATTGCCACTTCCTAGCTGCGATATGCCCAGATTGTTTTAAGCCAGCCAAAAACAAA CAGTCTGTATTCACTAGAATGGCAGTTATGAAAGCCTTGAATAAGATAAAGGAAGAGGAT TTCCTTAAGCAGTTTCCTTGTCCTCCAAACTCACCAAAGGCTGTATGCGCTGTTCTTGAA ATTGAATGTGCTCATGGTGCTGTTTTTGTGGCTGGGAGATATAATAAATACTCCAGGAAT CTACCACAAACTCCTTGGATAATTGATGGAGAAAGGAAGCTGGAATCTTCAGTGGAAGAA TTAATTTCAGATCATCTGTTGGCAGTATTTAAAGCAGAGAGTTTTAATTTTTCATCCTCT GGAAGAGAAGATGTAGATGTGAGAACATTAGGAAATGGAAGGCCCTTTGCAATTGAGCTG GTGAATCCTCATAGAGTACATTTCACTTCACAAGAAATTAAGGAACTTCAGCAGAAAATT AATAACTCATCTAACAAAATCCAAGTACGTGACTTGCAGCTTGTCACAAGAGAGGCAATA GGACATATGAAAGAAGGTGAAGAAGAAAAGACAAAGACCTACAGTGCCTTAATTTGGACA AATAAAGCGATACAGAAGAAAGACATTGAATTCCTAAATGACATAAAGGACTTAAAGATC GACCAGAAAACACCTTTGCGCGTCCTTCACCGAAGGCCCCTGGCTGTGCGAGCTCGCGTC ATTCACTTCATGGAGACACAGTACGTGGATGAGCACCACTTCCGCCTCCACTTGAAAACT CAGGCTGGCACCTACATTAAAGAGTTTGTACATGGAGACTTCGGGAGAACCAAGCCAAAC ATTGGGTCCCTGATGAATGTGACTGCAGACATTCTGGAGCTGGATGTTGAGTCTGTAGAT GTTGACTGGCCACCTGCTCTGGATGACTAG Clone variation with respect to NM_144709.2 1317 a=>g >OriGene 5' read for NM_144709 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTGTTAGCAA AGGAGGGTTATAATTATTCAATATGTTCCCACTGACTGAGGAAAACAAGCATGTGGCCCA GTTGTTGCTCAATACTGGTACTTGTCCAAGATGTATCTTCAGATTCTGTGGTGTGGATTT TCATGCACCTTACAAACTTCCATACAAGGAGTTGCTCAATGAACTACAGAAATTTCTGGA AACTGAAAAAGATGAATTAATTTTGGAAGTTATGAACCCACCTCCCAAGAAAATTCGACT GCAAGAACTGGAAGATAGTATTGATAATCTAAGTCAAAATGGAGAGGGAAGGATCTCTGT TAGTCATGTTGGAAGCACTGCTTCCAAGAACTCAAATTTAAATGTATGTAATGTATGCCT AGGAATTCTTCAAGAATTCTGTGAGAAAGATTTCATTAAAAAGGTGTGCCAAAAGGTTGA GGCCTCTGGGTTTGAATTCACCAGCTTGGTATTTTCAGTCTCCTTCCCACCACAACTATC TGTAAGAGAGCATGCTGCATGGTTGCTGGTAAAACAGGAAATGGGAAAGCAGAGTCTGTC GCTGGGAAGAGATGATATAGTTCAGCTAAAAGAAGCCTACAAATGGATAACTCACCCCCT GTTTTCAGAGGAACTGGGTGTTCCCATTGATGGAAAGAGCTTGTTTGAAGTGAGTGTGGT CTTTGCTCACCCAGAAAACAGTTGAGATTGCCACTTCCTAGCTGCGATATGCCCAGATTG TTNTAAGCCAGCCAAAAACAACAGTCTGTATTCACTAGAATGGCACGTATGAAAGCCCTG TATAAGATAAAGAAGAAGATTNCCTTAGCAGTTTCCTGTCCTCCAACTCACAAAGCTGTT GCA >OriGene 3' read for NM_144709 unedited
GTACACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTT AACTATATAAGATTGTATTAAATAGTCTTGGGCTATTTTTTGGTAAGCTCTTTGATTTGG TAACTACATTCCAAAAGTTAATTATCAGTTACATGAGTTTAAATGGCATCCATAGCTGCC CCTTCACACAAACAGGTGCTCAGTAATAGGGCTTATTAGAAAGACCCTAGAAACCAAGAA ACTTCAGTTCTAGTCCTCAATATAGAAGTAGCTAACAATTTTGGATAAGTCATTTAACTT CTAGTCTTTGGTTTCCATATTTATAAAATGGAGACAATATCCTATCTACTTTAATAAGGT ATTATTAAAATGTTTATAATTGGATAATATATTGAAGCATTCTGAAATAATACCAGTTCG TCTTTGTTTAGCAATCTCAATTTTTCAAATTCTTAACCCTGGGATTGAAGTTATTAAAGG CACTTGTTTCTCCTTATTTAAAAGATTTATGAAAGGCTCCTACCATGGCATGCCTTGGCT CCTACGGTTTTACTTTCCCTCCACAGGAAACCTATATTGACAATTTTAGATTGTCAAAAT TCTTTCTTAATGAAAGTATTAATGAGAATTATTTCATTATTCATAGTTTGGTTAAGATTT ATGACAAATCACAGCCAAAAGAACTTCATTGGTAATAGCTTGTTTGAGGCTTCCTGGGGA AAATAATGATGTCAGATTTGCATTTGCAATCTTAGACTATTATAGGATATTATGCTGCAT AAATCTAGATATAACTTTTATTCAGATAATTTACAAAAATTAGAACATGATTTTAAAGGT GGTATATCTCTTGTGGAGGGAAATCTTGCTGAAATACAGCTTCTTAGGTTACAGAGAATG TGTCCTGTTTGGCACCTCTCTAATGAAAGAATTANGAGAATATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_144709 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144709.1, NP_653310.1 |
| RefSeq Size | 2097 bp |
| RefSeq ORF | 1590 bp |
| Locus ID | 150962 |
| UniProt ID | Q3MIT2 |
| Gene Summary | Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210979 | PUS10 (Myc-DDK-tagged)-Human pseudouridylate synthase 10 (PUS10) |
CNY 3936.00 |
|
| RC210979L3 | Lenti ORF clone of Human pseudouridylate synthase 10 (PUS10), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC210979L4 | Lenti ORF clone of Human pseudouridylate synthase 10 (PUS10), mGFP tagged |
CNY 5890.00 |
|
| RG210979 | PUS10 (tGFP-tagged) - Human pseudouridylate synthase 10 (PUS10) |
CNY 4370.00 |
