LRRC8B (NM_015350) Human Untagged Clone
CAT＃: SC120691
LRRC8B (untagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1
CNY 8832.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TA-LRRP; TALRRP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120691 sequence for NM_015350 edited (data generated by NextGen Sequencing)
ATGATTACACTAACTGAGCTAAAATGCTTAGCAGATGCCCAGTCATCTTATCACATCTTA AAACCATGGTGGGACGTCTTCTGGTATTACATCACACTGATCATGCTGCTGGTGGCCGTG CTGGCCGGAGCTCTCCAGCTGACGCAGAGCAGGGTTCTGTGCTGTCTTCCATGCAAAGTG GAATTTGACAATCACTGTGCCGTGCCTTGGGACATCCTGAAAGCCAGCATGAACACATCC TCTAATCCTGGGACACCGCTTCCGCTCCCCCTCCGAATTCAGAATGACCTCCACCGACAG CAGTACTCCTATATTGATGCCGTCTGTTACGAGAAACAGCTCCATTGGTTTGCAAAGTTT TTCCCCTATCTGGTGCTCTTGCACACGCTCATCTTTGCAGCCTGCAGCAACTTTTGGCTT CACTACCCCAGTACCAGTTCCAGGCTCGAGCATTTTGTGGCCATCCTTCACAAGTGCTTC GATTCTCCATGGACCACCCGCGCCCTTTCAGAAACAGTGGCTGAGCAGTCAGTGAGGCCT CTGAAACTCTCCAAGTCCAAGATTTTGCTTTCGTCCTCAGGGTGTTCAGCTGACATAGAT TCCGGCAAACAGTCATTGCCCTACCCACAGCCAGGTTTGGAGTCAGCTGGCATAGAAAGC CCAACTTCCAGTGTCCTGGACAAGAAGGAGGGTGAACAGGCCAAAGCCATCTTTGAAAAA GTGAAAAGATTCCGCATGCATGTGGAGCAGAAGGACATCATTTATAGAGTATATCTGAAA CAGATAATAGTCAAAGTCATTTTGTTTGTGCTCATCATAACTTATGTTCCATATTTTTTA ACCCACATCACTCTTGAAATCGACTGTTCAGTTGATGTGCAGGCTTTTACAGGATATAAG CGCTACCAGTGTGTCTATTCCTTGGCAGAAATCTTTAAGGTCCTGGCTTCATTTTATGTC ATTTTGGTTATACTTTATGGTCTGACCTCTTCCTACAGCCTGTGGTGGATGCTGAGGAGT TCCCTGAAGCAATATTCCTTTGAGGCGTTAAGAGAAAAAAGCAACTACAGTGACATCCCT GATGTCAAGAATGACTTTGCCTTCATCCTTCATCTGGCTGATCAGTATGATCCTCTTTAT TCCAAACGCTTCTCCATATTCCTATCAGAGGTCAGTGAGAACAAACTGAAACAGATCAAC CTCAATAATGAATGGACAGTTGAGAAACTGAAAAGTAAGCTTGTGAAAAATGCCCAGGAC AAGATAGAACTGCATCTTTTTATGCTCAACGGTCTTCCAGACAATGTCTTTGAGTTAACT GAAATGGAAGTGCTAAGCCTGGAGCTTATCCCAGAGGTGAAGCTGCCCTCTGCAGTCTCA CAGCTGGTCAACCTCAAGGAGCTTCGTGTGTACCATTCATCTCTGGTCGTAGACCATCCT GCACTGGCCTTTCTAGAGGAGAATTTAAAAATCCTCCGCCTGAAATTTACTGAAATGGGA AAAATCCCACGCTGGGTATTTCACCTCAAGAATCTCAAGGAACTTTATCTTTCGGGCTGT GTTCTCCCTGAACAGTTGAGTACTATGCAGTTGGAGGGCTTTCAGGACTTAAAAAATCTA AGGACCCTGTACTTGAAGAGCAGCCTCTCCCGGATCCCACAAGTTGTTACAGACCTCCTG CCTTCATTGCAGAAACTGTCCCTTGATAATGAGGGAAGCAAACTGGTTGTGTTGAACAAC TTGAAAAAGATGGTCAATCTGAAAAGCCTAGAACTGATCAGCTGTGACCTGGAACGCATC CCACATTCCATTTTCAGCCTGAATAATTTGCATGAGTTAGACCTAAGGGAAAATAACCTT AAAACTGTGGAAGAGATCATTAGCTTTCAGCATCTTCAGAATCTTTCCTGCTTAAAGTTG TGGCACAATAACATTGCTTATATTCCTGCACAGATTGGGGCATTATCTAACCTAGAGCAG CTCTCTTTGGACCATAATAATATTGAGAATCTGCCCTTGCAGCTTTTCCTATGCACTAAA CTACATTATTTGGATCTAAGCTATAACCACTTGACCTTCATTCCAGAAGAAATCCAGTAT CTGAGTAATTTGCAGTACTTTGCTGTGACCAACAACAATATTGAGATGCTACCAGATGGG CTGTTTCAGTGCAAAAAGCTGCAGTGTTTACTTTTGGGGAAAAATAGCTTGATGAATTTG TCCCCTCATGTGGGTGAGCTGTCAAACCTTACTCATCTGGAGCTCATTGGTAATTACCTG GAAACACTTCCTCCTGAACTAGAAGGATGTCAGTCCCTAAAACGGAACTGTCTGATTGTT GAGGAGAACTTGCTCAATACTCTTCCTCTCCCTGTAACAGAACGTTTACAGACGTGCTTA GACAAATGTTGA Clone variation with respect to NM_015350.2 >OriGene 5' read for NM_015350 unedited
NGGGAGTTCACTTTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGGG CTGGAGTCGCGCAGGTAATAGTTAACCTCTTCTGTGAGAAGTCAGAAGGTGATCTCTTTA ATGCTTTCTTTTTAAGAATTTTTCAAATTGAGACTAATTGCAGAGGTTCCAGTTGACCAG CATTCATAGGAATGAAGACAAACACAGAGATGGTGTGTCTAAGAAACTTCAAAAGGTGTA GACCTCCTGACTGAAGCATATTGGATTTATTTAATTTTTTTCACTGTATTTCTGTCCTCC TACAAGGGAAAGTCATGATTACACTAACTGAGCTAAAATGCTTAGCAGATGCCCAGTCAT CTTATCACATCTTAAAACCATGGTGGGACGTCTTCTGGTATTACATCACACTGATCATGC TGCTGGTGGCCGTGCTGGCCGGAGCTCTCCAGCTGACGCAGAGCAGGGTTCTGTGCTGTC TTCCATGCAAAGTGGAATTTGACAATCACTGTGCCGTGCCTTGGGACATCCTGAAAGCCA GCATGAACACATCCTCTAATCCTGGGACACCGCTTCCGCTCCCCCTCCGAATTCAGAATG ACCTCCACCGACAGCAGTACTCCTATATTGATGCCGTCTGTTACGAGAAACAGCTCCATT GGTTTGCAAAGTTTTTCCCCTATCTGGTGCTCTTGCACACGCTCATCTTTGCAGCCTGCA GCAACTTTTGGCTTCACTACCCCAGTACCAGTTCCAGGCTCGAGCATTNTGTGGCCATTC TTCACAAGTGCTTCGATTCTTCATGGACCACCCGCGCCCTTTCAGAAACAGTGGCTGAGC AGTCAGTGAGGCCTCTGAAACTCTNCAGTCCAAGATTTGCTTTCGTCTTCAGC >OriGene 3' read for NM_015350 unedited
CTANGATCGATTTTTTTTTTTTTTTTTTGAGAAAGAGTCTTGCTCTGTCACCCAGGCTGG TGTACAGTGGCACAATCTTGGCTCACTGCAACCTCCACCTCCCTGGTTCAAGCAATTCCC CTGCGTCAGCCTCCCAAGTAGCTGGGATTACAGGCGCACGCCACCACGCCTGGCTAATTT TTTTGTAGTTTTAGCAGAGATGGGGTTTCACCAGGTTGGCCAGACTGGTCTCGAACTCCT GACCTCAAGCAATCCGCCCATCTTGGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCCA CCACGCCCGGCCGAGCATACTTTTAAAAATGATTTTGAAACACGGGTCTCTTTTCTTTAA GTCAACATTTGTCTAAGCACGTCTGTAAACGTTCTGTTACAGGGAGAGGAAGAGTATTGA GCAAGTTCTCCTCAACAATCAGACAGTTCCGTTTTAGGGACTGACATCCTTCTAGTTCAG GAGGAAGTGTTTCCAGGTAATTACCAATGAGCTCCAGATGAGTAAGGTTTGACAGCTCAC CCACATGAGGGGACAAATTCATCAAGCTATTTTTCCCCAAAAGTAAACACTGCAGCTTTT TGCACTGAAACAGCCCATCTGGTAGCATCTCAATATTGTTGTTGGTAACAGCAAAGTACT GCAAATTACTCAGATACTGGATTTCTTCTGGAATGAAAGTCAAGTGGTTATAGCTTAAAT CCAAATAATGTAGTTTAGTGCATAGGAAAAGCTGCAAGGGCAGATTCTCAATATATTATG GTCCAAAAAGAGCTGCTCTTAGTTTAGATAAAGCCCCCAATCTGTGCAGATATATAACCA ATGGTATTGTGCCACAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015350 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015350.1, NP_056165.1 |
| RefSeq Size | 3588 bp |
| RefSeq ORF | 2412 bp |
| Locus ID | 23507 |
| UniProt ID | Q6P9F7 |
| Domains | LRR |
| Protein Families | Transmembrane |
| Gene Summary | Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205553 | LRRC8B (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1 |
CNY 8824.00 |
|
| RC205553L1 | Lenti-ORF clone of LRRC8B (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1 |
CNY 11224.00 |
|
| RC205553L2 | Lenti-ORF clone of LRRC8B (mGFP-tagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1 |
CNY 5890.00 |
|
| RC205553L3 | Lenti-ORF clone of LRRC8B (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1 |
CNY 5890.00 |
|
| RC205553L4 | Lenti-ORF clone of LRRC8B (mGFP-tagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1 |
CNY 11224.00 |
|
| RG205553 | LRRC8B (tGFP-tagged) - Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1 |
CNY 10424.00 |
