KBTBD8 (NM_032505) Human Untagged Clone
CAT＃: SC120690
KBTBD8 (untagged)-Human kelch repeat and BTB (POZ) domain containing 8 (KBTBD8)
CNY 4288.00
CNY 9690.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TA-KRP; TAKRP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_032505, the custom clone sequence may differ by one or more nucleotides
ATGGCCGCGTCGGCAGATTTAAGTAAGTCTTCCCCAACACCGAATGGGATTCCATCTTCAGACCCAGCCA GCGATGCCATGGACCCCTTCCATGCTTGCAGTATTCTTAAGCAACTCAAAACAATGTACGATGAAGGACA GTTGACAGACATTGTAGTGGAAGTGGATCACGGGAAAACATTTTCCTGTCATAGAAACGTTCTTGCTGCA ATCAGCCCTTACTTCAGATCCATGTTCACTAGCGGCCTTACAGAAAGTACTCAAAAAGAAGTTCGAATAG TTGGTGTTGAAGCTGAATCGATGGATTTAGTGTTGAACTATGCCTACACTTCCAGAGTTATTCTTACAGA GGCCAATGTTCAAGCCTTGTTCACTGCAGCTAGCATCTTCCAGATTCCTTCCATCCAAGACCAATGTGCT AAGTATATGATCAGTCATTTGGACCCACAGAATTCTATTGGGGTCTTTATCTTTGCTGATCATTATGGTC ATCAGGAACTCGGAGATCGATCAAAAGAATACATTCGTAAAAAGTTTCTGTGTGTCACCAAAGAACAAGA GTTTCTCCAGTTGACAAAAGACCAACTGATAAGTATACTAGACAGTGACGATTTAAATGTAGACCGAGAA GAGCATGTTTATGAAAGCATTATAAGGTGGTTTGAGCATGAACAGAATGAAAGAGAAGTGCACCTTCCAG AAATTTTTGCTAAATGCATACGTTTTCCTCTGATGGAAGATACCTTTATAGAGAAAATTCCACCTCAGTT TGCACAGGCTATAGCCAAAAGCTGTGTAGAAAAGGGACCATCCAACACCAATGGCTGTACACAGAGGCTT GGAATGACTGCTTCTGAAATGATCATATGTTTTGATGCTGCCCACAAACACTCAGGAAAGAAGCAAACAG TGCCTTGTCTAGATATAGTCACAGGAAGGGTGTTTAAACTATGCAAACCACCAAATGACCTGAGAGAAGT TGGGATTCTTGTATCACCAGATAATGACATTTACATTGCAGGAGGGTACAGGCCAAGCAGCAGTGAGGTC TCCATCGACCATAAGGCAGAAAATGATTTCTGGATGTATGATCATTCCACCAATAGATGGCTATCCAAAC CATCCTTGCTTCGAGCCAGAATAGGCTGCAAACTTGTCTATTGCTGTGGTAAAATGTATGCAATCGGAGG TCGTGTTTATGAAGGTGATGGGAGAAACTCACTAAAATCTGTTGAGTGCTACGACAGTAGAGAGAATTGT TGGACGACTGTTTGCGCGATGCCAGTTGCAATGGAATTTCATAATGCTGTGGAGTACAAAGAGAAGATCT ATGTTTTACAGGGAGAATTTTTTCTCTTCTATGAGCCTCAAAAAGACTACTGGGGTTTCTTAACCCCCAT GACTGTGCCTAGAATCCAGGGCTTAGCAGCTGTATACAAGGACTCTATCTACTACATAGCTGGAACCTGT GGAAATCATCAACGTATGTTTACTGTAGAAGCCTATGATATTGAGCTAAATAAATGGACTCGTAAGAAAG ACTTTCCATGTGATCAGTCCATAAATCCATACCTTAAACTGGTACTTTTCCAGAACAAACTCCATTTATT TGTTCGAGCTACTCAAGTGACTGTTGAAGAACACGTCTTCAGAACCAGCAGAAAAAATTCCCTTTACCAA TATGATGACATTGCTGACCAGTGGATGAAAGTGTATGAGACCCCAGATCGGCTCTGGGACCTTGGCCGGC ATTTTGAATGTGCTGTTGCTAAACTGTATCCTCAGTGTCTTCAGAAAGTACTCTAA >OriGene 5' read for NM_032505 unedited
TTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCGATGCCGTAACTGGCTC GGCACTTCTTGTAGTTGTACGATTTTTCTCCTCCTAGATTTAAGTAAGTCTTCCCCAACA CCGAATGGGATTCCATCTTCAGACCCAGCCAGCGATGCCATGGACCCCTTCCATGCTTGC AGTATTCTTAAGCAACTCAAAACAATGTACGATGAAGGACAGTTGACAGACATTGTAGTG GAAGTGGATCACGGGAAAACATTTTCCTGTCATAGAAACGTTCTTGCTGCAATCAGCCCT TACTTCAGATCCATGTTCACTAGCGGCCTTACAGAAAGTACTCAAAAAGAAGTTCGAATA GTTGGTGTTGAAGCTGAATCGATGGATTTAGTGTTGAACTATGCCTACACTTCCAGAGTT ATTCTTACAGAGGCCAATGTTCAAGCCTTGTTCACTGCAGCTAGCATCTTCCAGATTCCT TCCATCCAAGACCAATGTGCTAAGTATATGATCAGTCATTTGGACCCACAGAATTCTATT GGGGTCTTTATCTTTGCTGATCATTATGGTCATCAGGAACTCGGAGATCGATCAAAAGAA TACATTCGTAAAAAGTTTCTGTGTGTCACCAAAGAACAAGAGTTTCTCCAGTTGACAAAA GACCAACTGANTAGTATACTAGACAGTGACGATTTAAATGTAGACCGAGAAGAGCATGTT TATGAAAGCATTATAAGGTGGGTTGAGCATGAACAGAATGAAAGAGAAGTGCACCTTCCA GAAATTTTTGCTAAATGCATACGTTTTCCTCTGATGGAAGATACCTTTTATAGAGAAATT NCACCTCAGTTTGCACAGGCTATAGCCAAAAGCTGGGTAGAAAGGGGACATTCAACACCA ATGGCTGTACCC >OriGene 3' read for NM_032505 unedited
AAATTTGGGNAAGGTTTTTATTTTGGAAAATGACATCAGTAAAAAGCAAAAAATGTGCAA TTATGGGCAAAATGTTTGAAAAATATCTACACATTTGCCCCCACAGNACTACAGTACTTA CTACATACCTGAGCACTGAACATTGAATTCCATTTTAAACTGCTTTACATAGGGAATCTG ATTCCTTCATGATCACATCCATTTGTTCTCATGACCAATAAAATCAGCTTGATGCTTAAG CATCAACTTTGNTAGAACAGCAAAACAAAGATGGAAAATAAAGAATACAATTTCTACTTT CCTATAACATAGTTATACCCAGTCCAGTTTTTCAATGTGGGACAAATTTTAGGGAAAGGG CTCCTACATTTTTAAAAGCAAAACCAAGTTAAAGGGAAACGGGCTTTTGGTAAACCCACA TTTAATTTTGGGAACCCCTGGGGCCTTTTATTAAGGGGTTCCCCCCCCCTTGAAAAGCCC CCAAACCTCAAAAAAAACCCCCCCGGGCCCCAATTCTCCTTTTTGGGGCCCCTTCGGGAC CCTTTTCAACGGCGGGAAAAACCCCCCCCCTTTTTCCTACAAAACCAATTTTTTTCTCCA AAAAAAAAAAAAATTTTTTTCCCCCCCCCCAAAAAAAATTCCCCCGCGTTGGTTTTAACC CCCCCACAAAACATCAACGCCCGCCCCCCTCTTCACAAAAATTGGGGCCGCCGCTCCACG ACAAAAACATCGGGCGCGGGGCCGCTCTACCAATAACATTAATTTCCTTGCTGTTTGAAA TCGCTCCCCCCATTCCCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032505 |
| Insert Size | 4540 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032505.1, NP_115894.1 |
| RefSeq Size | 4684 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 84541 |
| UniProt ID | Q8NFY9 |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification (PubMed:26399832). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229306 | KBTBD8 (Myc-DDK-tagged)-Human kelch repeat and BTB (POZ) domain containing 8 (KBTBD8) |
CNY 4472.00 |
|
| RC229306L3 | Lenti ORF clone of Human kelch repeat and BTB (POZ) domain containing 8 (KBTBD8), Myc-DDK-tagged |
CNY 6460.00 |
|
| RC229306L4 | Lenti ORF clone of Human kelch repeat and BTB (POZ) domain containing 8 (KBTBD8), mGFP tagged |
CNY 6460.00 |
|
| RG229306 | KBTBD8 (tGFP-tagged) - Human kelch repeat and BTB (POZ) domain containing 8 (KBTBD8) |
CNY 5040.00 |
|
| SC327941 | KBTBD8 (untagged)-Human kelch repeat and BTB (POZ) domain containing 8 (KBTBD8) |
CNY 9690.00 |
