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GTP cyclohydrolase 1 (GCH1) (NM_000161) Human Untagged Clone
CAT＃: SC120083
GCH1 (untagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 1
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYT5; DYT5a; DYT14; GCH; GTP-CH-1; GTPCH1; HPABH4B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120083 sequence for NM_000161 edited (data generated by NextGen Sequencing)
ATGGAGAAGGGCCCTGTGCGGGCACCGGCGGAGAAGCCGCGGGGCGCCAGGTGCAGCAAT GGGTTCCCCGAGCGGGATCCGCCGCGGCCCGGGCCCAGCAGGCCGGCGGAGAAGCCCCCG CGGCCCGAGGCCAAGAGCGCGCAGCCCGCGGACGGCTGGAAGGGCGAGCGGCCCCGCAGC GAGGAGGATAACGAGCTGAACCTCCCTAACCTGGCAGCCGCCTACTCGTCCATCCTGAGC TCGCTGGGCGAGAACCCCCAGCGGCAAGGGCTGCTCAAGACGCCCTGGAGGGCGGCCTCG GCCATGCAGTTCTTCACCAAGGGCTACCAGGAGACCATCTCAGATGTCCTAAACGATGCT ATATTTGATGAAGATCATGATGAGATGGTGATTGTGAAGGACATAGACATGTTTTCCATG TGTGAGCATCACTTGGTTCCATTTGTTGGAAAGGTCCATATTGGTTATCTTCCTAACAAG CAAGTCCTTGGCCTCAGCAAACTTGCGAGGATTGTAGAAATCTATAGTAGAAGACTACAA GTTCAGGAGCGCCTTACAAAACAAATTGCTGTAGCAATCACGGAAGCCTTGCGGCCTGCT GGAGTCGGGGTAGTGGTTGAAGCAACACACATGTGTATGGTAATGCGAGGTGTACAGAAA ATGAACAGCAAAACTGTGACCAGCACAATGTTGGGTGTGTTCCGGGAGGATCCAAAGACT CGGGAAGAGTTCCTGACTCTCATTAGGAGCTGA Clone variation with respect to NM_000161.2 >OriGene 5' read for NM_000161 unedited
GCGGCAACGCAATTCGCACGAGGCCCGGCTCGGTATGTGATCTAAGCAGGTCGCGTACCT TCCTCAGGTGACTCCGGCCACAGCCCATTGTCCGCGGCCACCGGCGGAGTTTAGCCGCAG ACCTCGAAGCGCCCCGGGGTCCTTCCCGAACGGCAGCGGCTGCGGCGGGTCCATGGAGAA TGGCTCNGTGCGGGCACCGGCGGAGAAGCCGCGGGGCGCCAGGTGCAGCAATGGGTTCCC CGAGCGGGATCCGCCGCGGCCCGGGCCCATCAGGCCGGCGGAGAAGCCCCCGCGGCCCGA GGCCAAGAGCGCGCAGCCCGCGGACGGCTGGAAGGGCGAGCGGCCCCGCAGCGAGGAGGA TAACGAGCTGAACCTCCCTAACCTGGCAGCCGCCTACTCGTCCATCCTGAGCTCGCTGGG CGAGAACCCCCAGCGGCAAGGGCTGCTCAAGACGCCCTGGAGGGCGGCCTCGGCCATGCA GTTCTTCACCAAGGGCTACCAGGAGACCATCTCAGATGTCCTAAACGATGCTATATTTGA TGAAGATCATGATGAGATGGTGATTGTGAAGGACATAGACATGTTTTCCATGTGTGAGCA TCACTTGGTTCCATTTGTTGGAAAGGTCCATATTGGTTATCTTCCTAACAGCAAGTCCTT GGCCTCAGCAAACTTGCGAGGATTGTAGAAATCTATAGTAGAAGACTACCAGTTCAGGAG CGCCTTACAAAACACATGGCTGTAGCAATCACGGAAGCCTTGCGGCCTGCTGGAGTCCGC GTAGTGGTTGAAGCAACACACATGTGTTTGGTAATGCGAGGTGTACACAAAATGACCAGC ANACTGTGACCAGCACAATGTTGGGTGTGTTCCCGGGAGGACC >OriGene 3' read for NM_000161 unedited
AATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAGTTACAATA GTTTAATAATTTAAATAGGACCAACTTCAGGAACATACATACTCATACATAAAATTAAAC AATTTAATTTTGAACAGTGTATTGAAATACATCAAATTCTTAAAAATCCCCCAAATGGAC TCAAGATCATGGATATGAAAAGGTAATTTTGAAGTACTAAAGACTAGAGTAAAACAGACA AAGTCATTACTTTGCATTTACTAATAAGACAACAGCCTGTGGATACATTAGACCTTTATA AGAACACTTCTAGGAAATGTTAGAACAACGAGTCATTAAAAAGGAATATAAATGAGTTCA TAAAGATAAATGTATAGCTGACAATTTCTTTGGTCCTCGAAGTCACACTTGTTTTTACTT TAAAATGCCCAACATGAGTTGAGTGCTCAGAATTCTACCTTATTAATGTCTTAATATTAA GCAAGCTAATGGTTATTCTGGGGCTAGATTTAAAAAGGGACTTCAGCCCACCCTCACTGG ACTTTAACTTCAGAAAGAAAAAGCCTGGGAACAGAAGTTGAGAGGAATGGGAAAAAACCA AAAAACCCCATTCCCTCTGCCATCTTGCCCCCATCATAACCCAAAATAGCATCCAAGTGG GCAGAGATGGGGACTAAAGTTTAAAGCAAGCATCAAAGTTATACTTTTTAGGGTAATGGG ATGAAATTTGAAGACACTATGTCAACCAATCCTAAACTCATGGATAACTGTGTTTGTGTT CTTGTGAGAGTTGCGGTTTTGTTGTTTAATTTGGCCACGCTGCCCCATGGAGGAATTTAA GATATCATTATTAGGCACAGCTTCCAGATTAAATACTATCCATCTTACGGAGTCCATGAG ACAGACCCCTTGACCCCAAGGAAGGGACCGAGCTTCATGCATTTCACGATCGTGTACATA CCTTGATAACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000161 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000161.2, NP_000152.1 |
| RefSeq Size | 2941 bp |
| RefSeq ORF | 753 bp |
| Locus ID | 2643 |
| UniProt ID | P30793 |
| Domains | GTP_cyclohydroI |
| Protein Families | Druggable Genome |
| Protein Pathways | Folate biosynthesis, Metabolic pathways |
| Gene Summary | This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1), also known as Type I, represents the longest transcript. Variants 1 and 2 encode the longest isoform (1), which is the active enzyme. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202893 | GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 1 |
CNY 3,600.00 |
|
| RC202893L1 | Lenti ORF clone of Human GTP cyclohydrolase 1 (GCH1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202893L2 | Lenti ORF clone of Human GTP cyclohydrolase 1 (GCH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202893L3 | Lenti ORF clone of Human GTP cyclohydrolase 1 (GCH1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202893L4 | Lenti ORF clone of Human GTP cyclohydrolase 1 (GCH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202893 | GCH1 (tGFP-tagged) - Human GTP cyclohydrolase 1 (GCH1), transcript variant 1 |
CNY 4,370.00 |
|
| SC320505 | GCH1 (untagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 1 |
CNY 2,400.00 |
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