PTEN (NM_000314) Human Untagged Clone

Name: PTEN (NM_000314) Human Untagged Clone
SKU: SC119965
Price: 5488.00

CAT＃: SC119965

PTEN (untagged)-Human phosphatase and tensin homolog (PTEN)

Datasheet

SDS

"NM_000314" in other vectors (6)

CNY 5488.00

货期*

现货

规格

Cited in 18 publications.

Product images

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Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; PTENbeta; TEP1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_000314 edited GAATTCGGCACGAGGGCCAAGCGGCGGCAGAGCGAGGGGCATCAGCTACCGCCAAGTCCA GAGCCATTTCCATCCTGCAGAAGAAGCCCCGCCACCAGCAGCTTCTGCCATCTCTCTCCT CCTTTTTCTTCAGCCACAGGCTCCCAGACATGACAGCCATCATCAAAGAGATCGTTAGCA GAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGACTTGACCTATATTTATCCAA ACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAGGAACAATATTG ATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATACAATCTTT GTGCTGAAAGACATTATGACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTG AAGACCATAACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAAT GGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAA CTGGTGTAATGATATGTGCATATTTATTACATCGGGGCAAATTTTTAAAGGCACAAGAGG CCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAAGGGAGTAACTATTCCCAGTC AGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAG TGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTT GCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGAC CCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCTGTGTGTG GTGATATCAAAGTAGAGTTCTTCCACAAACAGAACAAGATGCTAAAAAAGGACAAAATGT TTCACTTTTGGGTAAATACATTCTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTAG AAAATGGAAGTCTATGTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGATA ATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACAAAGCAAATAAAG ACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACAAAAACAG TAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTG ACAATGAACCTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAAC CTTTTGATGAAGATCAGCATACACAAATTACAAAAGTCTGAATTTTTTTTTATCAAGAGG GATAAAACACCATGAAAATAAACTTGAATAAACTGAAAATGGACCTTTTTTTTTTTAATG GCAATAGGACATTGTGTCAGATTACCAGTTATAGGAACAATTCTCTTTTCCTGACCAATC TTGTTTTACCCTATACATCCACAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAAGGTTG TGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGACATTTAAAATTCAATTAGGA TTAATAAAGATGGCACTTTCCCGTTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGA TGTGTATACGTAGGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAG CTTTGTGATATACTGGTTCACATCCTACCCCTTTGCACTTGTGGCAACAGATAAGTTTGC AGTTGGCTAAGAGAGGTTTCCGAAGGGTTTTGCTACATTCTAATGCATGTATTCGGGTTA GGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGGACTCTGGACCATATAC CATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTGGACATT CGAGGAATTGGCCGCTGTCACTGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGT GCTAGAAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGTACAGGAATGAACCTTCTGC AACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGTCATAGGTAAGAAACACAG CAACAATGACTTAACCATATAAATGTGGAGGCTATCAACAAAGAATGGGCTTGAAACATT ATAAAAATTGACAATGATTTATTAAATATGTTTTCTCAATTGTAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAACTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_000314 unedited ACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCCAAGCGGCGGCAGAGCGAGGGG CATCAGCTACCGCCAAGTCCAGAGCCATTTCCATCCTGCAGAAGAAGCCCCGCCACCAGC AGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAGACATGACAGCCA TCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAG ACTTGACCTATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAG GCGTATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACC ATTACAAGATATACAATCTTTGTGCTGAAAGACATTATGACACCGCCAAATTTAATTGCA GAGTTGCACAATATCCTTTTGAAGACCATAACCCACCACAGCTAGAACTTATCAAACCCT TTTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACT GTAAAGCTGGAAAGGGACGAACTGGTGTAATGATATGTGCATATTTATTACATCGGGGCA AATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGNGAAGTAAGGACCAGAGACAAAA GGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAT CATCTGGATTATAGACCAGTGGCACTGGNTGTTCACAAGATGATGTTTGAAACTATNCCC ATGGTCAGTGGCGGAACTTNGCATCCTCAGTTNGTGGTCTGCCAGCTAAGGGTGAAGAAT ATNCCTCCATNCAGGACCACACGACGGGNAGACAGTCATGTCCTTTGAGTCCCTCAGCCG TACCTGGGTGTGTGAATCAAAGAAAAGTCTTCCCAACAGACAGAGCTAAAAGGCAAATGN NTACTTTGGTAAAACATNTNATACAGACAAAGAACTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_000314 unedited ATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTACAATTGAGAAAAACATATTTAATAAATCATTGTCAATTTTTATAATGTTTCAAG CCCATTCTTTGTTGATAGCCTCCACATTTATATGGTTAAGTCATTGTTGCTGGGTTTCTT ACCTATGACATTATTTTTATATCCCTTCATTTGGGGATCTTAAAATGTTGCAAAAGGTTC ATTCCTGTACCCCAATACAGATTCACTTCCTTTAGCTGCCTTTTCTAGCACCAATATGCT TTAAAAAAAAATGCGCAAACAACAAGCAGGGACAGCGGCCAATTCCTCGAATGTCCAGAT TAATAACTGTAGCATGCTAAAGAAAGGGGTGTGTAAATAGCTGGAGATGGTATATGGTCC AGAGTCCAGCATAAAATTATTTCCTTTCTGAGCATTCCCTCCATTCCCCTAACCCGAATA CATGCATTAAAATGTAGCAAAACCCTTCGGAAACCTCTCTTAGCCAACTGCAAACTTATC TGTTGCCACAAGTGCAAAGGGGTAGGATGTGAACCAGTATATCACAAAGCTCTTTAGCCA CTTCAGTTGGTGACAGAACACAAAAGGAAAAAATTCCTACGTATACACATCAGTCTGTCT CCACTTTTTATAAAACTGGGATAAAACGGGAAAGNGCCATCTTTATTAATCCTAATTGAA TTTTAAATGCCCTTTTGACACAAAAGTATATACATGACACAGCTACACAACCTTTTTTCA ACTGGACANNCAGTGTCAAAACCCTGTGGTGTATAGGGTAAAACAAGATTGGTCAGGAAA GAGAATTGTTCCTAAACTGGGNATCTGACACAGTGCCTATTGCCATAAAAAAAAAGGGCC ATTTCAGTTATCCAAGTTATTTCATGGGGTTTATCCCTCTGAAAAAAAATCGACTTTGGA ATGGGATGCGGACTCACAAAGGTATCCCTGA
Restriction Sites	NotI-NotI
ACCN	NM_000314
Insert Size	2390 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_000314.3, NP_000305.3
RefSeq Size	3416 bp
RefSeq ORF	1212 bp
Locus ID	5728
UniProt ID	P60484
Domains	PTPc_motif
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction
Gene Summary	This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015] Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. The most abundant isoform (PTEN), which is derived from the use of the 5'-most AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (18)

The use of this cDNA Clones has been cited in the following citations:
A Direct Fluorometric Activity Assay for Lipid Kinases and Phosphatases ,Sun, J;Singaram, I;Soflaee, MH;Cho, W;, J. Lipid Res. ,PubMed ID 32341006 [PTEN]
Transcription factor CEBPB inhibits the proliferation of osteosarcoma by regulating downstream target gene CLEC5A ,Lu, J;Chen, W;Liu, H;Yang, H;Liu, T;, J. Clin. Lab. Anal. ,PubMed ID 31364785 [PTEN]
miR-3691-5p promotes hepatocellular carcinoma cell migration and invasion through activating PI3K/Akt signaling by targeting PTEN ,Du, W;Zhang, X;Wan, Z;, Onco Targets Ther ,PubMed ID 31417285 [PTEN]
Effect of miR-301a/PTEN pathway on the proliferation and apoptosis of cervical cancer ,Peng, LN;Shi, WT;Feng, HR;Wei, CY;Yin, QN;, Innate Immun ,PubMed ID 30943822 [PTEN]
Long noncoding RNA HULC accelerates liver cancer by inhibiting PTEN via autophagy cooperation to miR15a ,Xin, X;Wu, M;Meng, Q;Wang, C;Lu, Y;Yang, Y;Li, X;Zheng, Q;Pu, H;Gui, X;Li, T;Li, J;Jia, S;Lu, D;, Mol. Cancer ,PubMed ID 29895332 [PTEN]
SIX1 reduces the expression of PTEN via activating PI3K/AKT signal to promote cell proliferation and tumorigenesis in osteosarcoma. ,null, Biomedicine pharmacotherapy = Biomedecine pharmacotherapie ,PubMed ID 29807230 [PTEN]
MicroRNA-20b promotes cell proliferation via targeting of TGF-β receptor II and upregulates MYC expression in Ewing's sarcoma cells ,Kawano, M;Tanaka, K;Itonaga, I;Iwasaki, T;Tsumura, H;, Int. J. Oncol. ,PubMed ID 29039480 [PTEN]
Divergent Activity of the Pseudogene PTENP1 in ER-Positive and Negative Breast Cancer ,Yndestad, S;Austreid, E;Skaftnesmo, KO;Lønning, PE;Eikesdal, HP;, Mol. Cancer Res. ,PubMed ID 29021233 [PTEN]
MicroRNA-301a promotes cell proliferation via PTEN targeting in Ewing's sarcoma cells. ,null, International journal of oncology ,PubMed ID 26846737 [PTEN]
microRNA-93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells ,Kawano, M;Tanaka, K;Itonaga, I;Ikeda, S;Iwasaki, T;Tsumura, H;, J. Exp. Clin. Cancer Res. ,PubMed ID 26243299 [PTEN]
The virus-induced protein APOBEC3G inhibits anoikis by activation of Akt kinase in pancreatic cancer cells ,Wu, J;Pan, TH;Xu, S;Jia, LT;Zhu, LL;Mao, JS;Zhu, YL;Cai, JT;, Sci Rep ,PubMed ID 26178819 [PTEN]
miR-19a acts as an oncogenic microRNA and is up-regulated in bladder cancer ,Feng, Y;Liu, J;Kang, Y;He, Y;Liang, B;Yang, P;Yu, Z;, J. Exp. Clin. Cancer Res. ,PubMed ID 25107371 [PTEN]
Acid Ceramidase Promotes Nuclear Export of PTEN through Sphingosine 1-Phosphate Mediated Akt Signaling ,Beckham, TH;Cheng, JC;Lu, P;Marrison, ST;Norris, JS;Liu, X;, PLoS One. 2013 Oct 1;8(10):e76593 ,PubMed ID 24098536 [PTEN]
p34 is a novel regulator of the oncogenic behavior of NEDD4-1 and PTEN ,Hong, SW;Moon, JH;Kim, JS;Shin, JS;Jung, KA;Lee, WK;Jeong, SY;Hwang, JJ;Lee, SJ;Suh, YA;Kim, I;Nam, KY;Han, S;Kim, JE;Kim, KP;Hong, YS;Lee, JL;Lee, WJ;Choi, EK;Lee, JS;Jin, DH;Kim, TW;, Cell Death Differ. 2013 Oct 18. ,PubMed ID 24141722 [PTEN]
PI(4,5)P2 5-phosphatase A regulates PI3K/Akt signalling and has a tumour suppressive role in human melanoma ,null, Nature Communications ,PubMed ID 23443536 [PTEN]
Myc-Induced MicroRNAs Integrate Myc-Mediated Cell Proliferation and Cell Fate ,Jong Wook Kim, Seiichi Mori, and Joseph R. Nevins, Cancer Res., Jun 2010; 70: 4820 - 4828 [PTEN]
MicroRNA-21 Is a Downstream Effector of AKT That Mediates Its Antiapoptotic Effects via Suppression of Fas Ligand ,Danish Sayed, Minzhen He, Chull Hong, Shumin Gao, Shweta Rane, Zhi Yang, and Maha Abdellatif, J. Biol. Chem., Jun 2010; 285: 20281 - 20290 [PTEN]
IGF1/insulin signaling activates androgen signaling through direct interactions of FOXO1 with androgen receptor ,Wuqiang Fan, Toshihiko Yanase, Hidetaka Morinaga, Taijiro Okabe, Masatoshi Nomura, Hiroaki Daitoku, Akiyoshi Fukamizu, Shigeaki Kato, Ryoichi Takayanagi, and Hajime Nawata, J Biol Chem. 2007 Mar 9;282(10):7329-38. [PTEN]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202627	PTEN (Myc-DDK-tagged)-Human phosphatase and tensin homolog (PTEN)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3656.00 CNY 3990.00
RC202627L1	Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6056.00
RC202627L2	Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6056.00
RC202627L3	Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6056.00
RC202627L4	Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6056.00
RG202627	PTEN (tGFP-tagged) - Human phosphatase and tensin homolog (PTEN)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5256.00

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