RFX5 (NM_000449) Human Untagged Clone
CAT＃: SC119868
RFX5 (untagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1
CNY 4592.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000449, the custom clone sequence may differ by one or more nucleotides
ATGGCAGAAGATGAGCCTGATGCTAAGAGCCCCAAGACTGGGGGAAGGGCCCCCCCAGGTGGTGCTGAGG CTGGGGAACCTACCACCCTTCTTCAGAGGCTCCGAGGTACCATTTCCAAGGCCGTGCAGAACAAAGTAGA GGGGATCCTGCAAGATGTACAGAAATTTTCTGACAATGACAAGCTGTATCTCTACCTTCAGCTCCCCTCA GGACCCACCACTGGAGACAAAAGCTCAGAGCCAAGTACACTGAGCAATGAGGAGTACATGTATGCCTATA GGTGGATCCGCAACCACCTGGAAGAGCACACTGACACCTGTCTGCCAAAGCAAAGTGTTTATGATGCCTA TCGGAAGTACTGTGAGAGTCTTGCCTGTTGCCGCCCACTCAGCACAGCCAACTTTGGCAAGATCATCAGA GAGATCTTCCCTGACATCAAAGCTCGAAGGCTTGGTGGCCGGGGCCAGTCCAAATATTGCTACAGTGGCA TAAGGAGGAAGACCTTGGTGTCTATGCCACCCCTGCCTGGACTTGACCTAAAGGGTTCTGAGAGTCCAGA AATGGGCCCAGAAGTAACCCCAGCACCTCGAGATGAACTGGTGGAGGCAGCGTGTGCCCTGACCTGTGAC TGGGCAGAGCGGATCCTGAAACGGTCCTTCAGTTCCATCGTTGAGGTCGCCCGCTTCCTGCTACAGCAGC ATCTCATCTCTGCCCGATCTGCACATGCCCATGTGCTTAAGGCCATGGGGCTTGCTGAAGAGGACGAACA TGCACCTCGGGAACGGTCATCTAAACCAAAGAATGGTTTAGAGAACCCAGAGGGTGGAGCCCACAAGAAG CCAGAGAGACTGGCCCAGCCTCCTAAGGATCTGGAAGCCCGAACTGGGGCCGGTCCTCTCGCACGTGGAG AGCGGAAGAAGAGTGTAGTTGAGAGCTCGGCCCCAGGAGCCAATAACCTGCAGGTTAATGCCCTAGTGGC TCGGCTGCCTCTGCTCCTTCCCCGGGCCCCTCGCTCACTAATTCCGCCAATCCCAGTCTCTCCACCTATT CTGGCCCCCAGGCTTTCTTCAGGTGCCCTGAAAGTGGCTACACTGCCTCTGTCTAGTAGGGCCGGGGCAC CCCCAGCAGCTGTGCCCATCATTAACATGATCTTACCAACTGTTCCTGCTTTGCCTGGACCTGGACCTGG GCCTGGGCGAGCTCCACCTGGGGGACTCACTCAGCCCCGGGGCACAGAGAACAGAGAGGTAGGCATAGGT GGTGACCAAGGACCACATGACAAGGGTGTCAAGAGGACAGCTGAAGTACCTGTGAGTGAGGCCAGTGGGC AGGCTCCACCAGCTAAAGCAGCAAAGCAGGATATAGAGGATACAGCAAGTGATGCCAAAAGGAAACGGGG GCGCCCTCGAAAAAAGTCAGGTGGAAGTGGGGAAAGGAATTCTACCCCTCTCAAGTCAGCAGCTGCCATG GAATCTGCCCAGTCCTCAAGGTTACCATGGGAGACATGGGGCTCAGGAGGGGAAGGCAACTCAGCTGGAG GGGCAGAGAGGCCAGGGCCAATGGGAGAGGCTGAAAAGGGGGCAGTACTTGCCCAGGGTCAGGGAGATGG TACTGTTTCCAAAGGAGGAAGGGGCCCCGGTTCCCAGCATACCAAAGAAGCAGAAGATAAAATTCCCTTG GTCCCCTCAAAAGTGAGTGTCATCAAGGGCAGCAGAAGCCAAAAGGAGGCTTTTCCTTTGGCAAAGGGAG AGGTAGACACTGCACCACAGGGTAATAAAGACTTAAAGGAGCATGTGCTTCAAAGTTCCTTATCCCAGGA GCATAAAGACCCAAAAGCAACACCCCCATGA >OriGene 5' read for NM_000449 unedited
TGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGNAGAACTT GGGCCCAGTTTTTTTTCCAGCCTAGAAGGCAGAATATGTTCGTATCCTTTCTGACCTCCC AGGCATATGCAATGTTTTCCAGATTTAGGAGACTTCAGAAAGGTGGGGCAGATAGAATGG AGATGGCAAAGATCTCTTTGGGCATATATGGGCCTGGCGAAGTAATGGAATAATTTCTAA TTTTCGGAGAAGGCAAGTGCCCTCATGCCGGGATGGCAGAAGATGAGCCTGATGCTAAGA GCCCCAAGACTGGGGGAAGGGCCCCCCCAGGTGGTGCTGAGGCTGGGGAACCTACCACCC TTCTTCAGAGGCTCCGAGGTACCATTTCCAAGGCCGTGCAGAACAAAGTAGAGGGGATCC TGCAAGATGTACAGAAATTTTCTGACAATGACAAGCTGTATCTCTACCTTCAGCTCCCCT CAGGACCCACCACTGGAGACAAAAGCTCAGAGCCAAGTACACTGAGCAATGAGGAGTACA TGTATGCCTATAGGTGGATCCGCAACCACCTGGAAGAGCACACTGACACCTGTCTGCCAA AGCAAAGTGTTTATGATGCCTATCGGAAGTACTGTGAGAGTCTTGCCTGTTGCCGCCCAC TCAGCACAGCCAACTTTGGCAAGATCATCAGAGAGATCTTCCCTGACATCAAAGCTCGAA GGCTTGGTGGCCGGGGCCAGTCCAAATATTGCTACAGTGGCATAAAGAGGAAGACCTTGG TGTCTATGCCACCCCTGCCTGGACTTGACCTANAGGTTCTGAGAGTCCAGAAATGGGCCC AGAAGTACCCCCGCACCTCGAGATGAACTGGTGGGAGCAGCGTGTGCCCTGACTGTGACT GGGCAGAGCGGATCTCGAA >OriGene 3' read for NM_000449 unedited
GGGGGGGGGGGANAAANNNGANAANCTATTAACCCCCGGCCGGTCATCTCNCGCCTATTC TATGGGTTTCACTCAAGACCCAGGGTATCAAGCTGCAAAGGTCAGAAGGCAGCAACCAGG TACTAAGTAGACTGGGTGACTCAGCTGTCTGTACAGAGGAGAATGGACTTCCTTAGGAAA AGAATAGCCAAATGAGAAGCAAGTGCAAAGAAGGCCTCTACTAGGCAAAGTTAACGTAGG GATATAAACACTCTTCCCCACAGACCTGTATCATGGGGGTGTTGCTTTTGGGTCTTTATG CTCCTGGGATAAGGAACTTTGAAGCACATGCTCCTTTAAGTCTTTATTACCCTGTGGTGC AGTGTCTACCTCTCCCTTTGCCAAAGGAAAAGCCTCCTTTTGGCTTCTGCTGCCCTTGAT GACACTCACTTTTGAGGGGACCAAGGGAATTTTATCTTCTGCTTCTTTGGTATGCTGGGA ACCGGGGCCCCTTCCTCCTTTGGAAACAGTACCATTTCCCTGACCCTGTGCAAGTACTGC CCCCTTTTCAGCCTCTCCCATTGGCCCTGGCCTTTATTTCCCTCCAGCTGAACTTGCTTC CCCTTCTGAGCCCCATGTCTTCCATGGTAACCTTGAGGACTGGGCAGATTTCCATGGCAG CTGCTGACTTGAGAGGGGTAGAATTTCTTTCCCCACTTCCACCTGACTTTTTCCGAGGGC GCCCCCGATTACTTTTGGCTTCACTTGCTGTATCCTCTATTTCCTGTCTTGCCGCTTTAT ATGGTGCGAGCCTGGCCATTGGCTCTCATTACCAGGTACTTATCCTGTCTTTTTGTCACC CTTTACATGTGGCCCTTTGTTCCCACCTATCCCCACACTCCTGTTCTTCCTGTGCNCCCC GGCCTTATAGGAGTTCCTCAGTGGGCAGCTGTTCCTATCCCACGTTCTGGTCTATAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000449 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000449.3, NP_000440.1 |
| RefSeq Size | 3618 bp |
| RefSeq ORF | 1851 bp |
| Locus ID | 5993 |
| UniProt ID | P48382 |
| Domains | RFX_DNA_binding |
| Protein Families | Transcription Factors |
| Protein Pathways | Antigen processing and presentation, Primary immunodeficiency |
| Gene Summary | A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202368 | RFX5 (Myc-DDK-tagged)-Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1 |
CNY 4584.00 |
|
| RC202368L1 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1, Myc-DDK-tagged |
CNY 6984.00 |
|
| RC202368L2 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC202368L3 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202368L4 | Lenti ORF clone of Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG202368 | RFX5 (tGFP-tagged) - Human regulatory factor X, 5 (influences HLA class II expression) (RFX5), transcript variant 1 |
CNY 6184.00 |
