CDC27 (NM_001256) Human Untagged Clone
CAT＃: SC119338
CDC27 (untagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 2
CNY 6040.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119338 sequence for NM_001256 edited (data generated by NextGen Sequencing)
ATGACGGTGCTGCAGGAACCCGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCT TACCGAGATGCGGTTTTCCTCGCAGAACGCCTTTATGCAGAAGTACACTCAGAAGAAGCC TTGTTTTTACTGGCAACCTGTTATTACCGCTCAGGAAAGGCATATAAAGCATATAGACTC TTGAAAGGACACAGTTGTACTACACCGCAATGCAAATACCTGCTTGCAAAATGTTGTGTT GATCTCAGCAAGCTTGCAGAAGGGGAACAAATCTTATCTGGTGGAGTGTTTAATAAGCAG AAAAGCCATGATGATATTGTTACTGAGTTTGGTGATTCAGCTTGCTTTACTCTTTCATTG TTGGGACATGTATATTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAG AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCATTATGTGAAATAGGTGAA AAGCCAGATCCTGACCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAACTGTCTG CCCAACTCTTGCACAACACAAGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACA GTTCTTACGGAAACACCCCAGGACACAATTGAATTAAACAGATTGAATTTAGAATCTTCC AATTCAAAGTACTCCTTGAATACAGATTCCTCAGTGTCTTATATTGATTCAGCTGTAATT TCACCTGATACTGTCCCACTGGGAACAGGAACTTCCATATTATCTAAACAGGTTCAAAAT AAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAGCAGCTCTTAGTCCATTAACCCCA AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAAC TACACTAATACACCTCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAAAAAGTCT GTTGCCAGAATCGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGCCGA GAGGTAACTCCAATTCTTGCACAAACACAAAGTTCTGGTCCACAAACAAGTACAACACCT CAGGTATTGAGCCCCACTATTACATCTCCCCCAAACGCACTGCCTCGAAGAAGTTCACGA CTCTTTACTAGTGACAGCTCCACAACCAAGGAGAATAGCAAAAAATTAAAAATGAAGTTT CCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGAATAACTCAA CCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTTCCATCATTTCAGAAGGG AAAATATCCACAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGCAGCNNNA GGTTTGATGAGCCTTCTTCGTGAAATGGGGAAAGGTTATTTAGCTTTGTGTTCATACAAC TGCAAAGAAGCTATAAATATTTTGAGCCATCTACCTTCTCACCACTACAATACTGGTTGG GTACTGTGCCAAATTGGAAGGGCCTATTTTGAACTTTCAGAGTACATGCAAGCTGAAAGA ATATTCTCAGAGGTTAGAAGGATTGAGAATTATAGAGTTGAAGGCATGGAGATCTACTCT ACAACACTTTGGCATCTTCAAAAAGATGTTGCTCTTTCAGTTCTGTCAAAAGACTTAACA GACATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGAACTGTTTCAGTCTGCAA CGGGAACATGATATTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCCAAATTAC GCTTATGCCTATACTCTATTAGGGCATGAGTTTGTCTTAACTGAAGAATTGGACAAAGCA TTAGCTTGTTTTCGAAATGCTATCAGAGTCAATCCTAGACATTATAATGCATGGTATGGT TTAGGAATGATTTATTACAAGCAAGAAAAATTCAGCCTTGCAGAAATGCATTTCCAAAAA GCGCTTGATATCAACCCTCAAAGTTCAGTTTTACTTTGCCACATTGGAGTAGTTCAACAT GCACTGAAAAAATCAGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTCATTGATCCC AAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAAAATATAAG TCTGCTTTACAAGAACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCCCTCGTTTAC TTCTTAATAGGAAAGGTTTACAAGAAGTTAGGTCAAACGCACCTCGCCCTGATGAATTTC TCTTGGGCTATGGATTTAGATCCTAAAGGAGCCAATAACCAGATTAAAGAGGCAATTGAT AAGCGTTATCTTCCAGATGATGAGGAGCCAATAACCCAAGAAGAACAGATCATGGGAACA GATGAATCCCAGGAGAGCAGCATGACAGATGCGGATGACACACAACTTCATGCAGCTGAA AGTGATGAATTTTAA Clone variation with respect to NM_001256.3 1377 a=>n;1378 g=>n;1379 a=>n >OriGene 5' read for NM_001256 unedited
ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCGGTAACGGTCGCTGGT GAGTTTAAATGAGCAGGGGCTGGCCGGGCCGGAGCCGCTACAGGGGGGGCCTGAGGCACT GCAGAAAGTGGGCCTGAGCCTCGAGGATGACGGTGCTGCAGGAACCCGTCCAGGCTGCTA TATGGCAAGCACTAAACCACTATGCTTACCGAGATGCGGTTTTCCTCGCAGAACGCCTTT ATGCAGAAGTACACTCAGAAGAAGCCTTGTTTTTACTGGCAACCTGTTATTACCGCTCAG GAAAGGCATATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACTACACCGCAATGCA AATACCTGCTTGCAAAATGTTGTGTTGATCTCAGCAAGCTTGCAGAAGGGGAACAAATCT TATCTGGTGGAGTGTTTAATAAGCAGAAAAGCCATGATGATATTGTTACTGAGTTTGGTG ATTCAGCTTGCTTTACTCTTTCATTGTTGGGACATGTATATTGCAAGACAGATCGGCTTG CCAAAGGATCAGAATGTTACCAAAAGAGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCT TTGAATCATTATGTGAAATAGGTGAAAAGCCAGATCCTGACCAAACATTTAAATTCACAT CTTTACAGAACTTTAGCAACTGTCTGCCCAACTCTTGCACAACACAAGTACCTAATCATA GTTTATCTCACAGACAGCCTGAGACAGTTCTTACGGAAACACCCCAGGACACAATTGAAT TAAACAGATTGAATTTAGAAATCTTCAATTCAAAGTACTCCTTGAATACAGATTCCTCAG TGGTCTATATTGATNCAGCTGTAATTCACCTGATACTGTNCCACTGGGAACAGGAACTTC CATATATCTAAACAGGNTCANAATAACCAAAACTGGNTCGAAGNTATTANGAGGACAGCA GCTCTTAGTCCC >OriGene 3' read for NM_001256 unedited
GCCGCTTTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTGTTAT AGGCAACGTCTTTTATTGTTCCATCTGGCACTTGCAAACACCATTCTTGAAAAACCTAAA AATGAAGGTCAGTTACTCTGTTTATCAGGAACAGAACCAATTAAAAACAATTTTTTGAAA AAGCAACCAAAAAACTCTGGTTCATCACTATTTTCCATATCTAATGGTTCCTTCAAAATA AAGCATAACTCTTCCTTTCATGATACTTTCCAACAAAGGGAGATTAAAGAAAAACAGAAA AGAAAGTTCCCCACCCTACCCCCCATAAATGGTCATTCATACTGGAAAAAAAGCCAGTCT TGTTAGCAGCTAAATATTGCACTGCCTTTCATTCTGTATACAGCCAGGGTCCAATGAAAG GGGCACACTCATGGTTTAAGGGACGGACGATGACACCGCCAGCTCAAGAGTAAAGACTCA GTTTCCAGAGGGACAAGAAACACGTCAGCACTATTCACACATCCAGTTGTAAAAGCCTGA TTTCCAGAAGTTAAAATTCATCACTTTCACCTGCATGAAGTTGTGGGTCTTCCGCATTTG TTATGCTGCTCTCCTGGGATTCATCTGTCCCCATGATCTGTTCCTTCTTGGGTTATTGGC TCCTCATCATTCGGAAAAAACGCTAATCAATGGCCTCTTTATTTGGTATTTGCCTCCTTT AGGATCAAAATCCAACCCCAAAAAATTTCTCAGGGCGAGGGCCGTGGACCTTATTCTGTG AAACCTTTCCTTTAATAATAAACGAGGACTCTTTGGAACATATGTTCAATTTTCAATTCT TGAAAACCACTTTTCTTTTTTTGTCAAAAAATGAGCCCTGGGATTTTCATAAGGGTTTCG GAAAAATAACAGGTCTTGTTAGGGTCCAAACCCTCTTGTTTTTACGCGCGCGTACCTCAC AGGGCACATAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001256 |
| Insert Size | 3180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001256.2, NP_001247.2 |
| RefSeq Size | 3277 bp |
| RefSeq ORF | 2475 bp |
| Locus ID | 996 |
| UniProt ID | P30260 |
| Domains | TPR |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213034 | CDC27 (Myc-DDK-tagged)-Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 2 |
CNY 9024.00 |
|
| RC213034L3 | Lenti ORF clone of Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 2, Myc-DDK-tagged |
CNY 14820.00 |
|
| RC213034L4 | Lenti ORF clone of Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 2, mGFP tagged |
CNY 14820.00 |
|
| RG213034 | CDC27 (tGFP-tagged) - Human cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 2 |
CNY 10624.00 |
