ECH1 (NM_001398) Human Untagged Clone
CAT＃: SC119261
ECH1 (untagged)-Human enoyl CoA hydratase 1, peroxisomal (ECH1)
CNY 2400.00
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HPXEL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119261 sequence for NM_001398 edited (data generated by NextGen Sequencing)
ATGGCGGCGGGGATAGTGGCTTCTCGCAGACTCCGCGACCTACTGACCCGGCGACTGACA GGCTCCAACTACCCGGGACTCAGTATTAGCCTTCGCCTCACTGGCTCCTCTGCACAAGAG GCGGCTTCCGGAGTAGCCCTCGGTGAAGCCCCAGACCACAGCTATGAGTCCCTTCGTGTG ACGTCTGCGCAGAAACATGTTCTGCATGTCCAGCTCAACCGGCCCAACAAGAGGAATGCC ATGAACAAGGTCTTCTGGAGAGAGATGGTAGAGTGCTTCAACAAGATTTCGAGAGACGCT GACTGTCGGGCGGTGGTGATCTCTGGTGCAGGAAAAATGTTCACTGCAGGTATTGACCTG ATGGACATGGCTTCGGACATCCTGCAGCCCAAAGGAGATGATGTGGCCCGGATCAGCTGG TACCTCCGTGACATCATCACTCGATACCAGGAGACCTTCAACGTCATCGAGAGGTGCCCC AAGCCCGTGATTGCTGCCGTCCATGGGGGCTGCATTGGCGGAGGTGTGGACCTTGTCACC GCCTGTGACATCCGGTACTGTGCCCAGGATGCTTTCTTCCAGGTGAAGGAGGTGGACGTG GGTTTGGCTGCCGATGTAGGAACACTGCAGCGCCTGCCCAAGGTCATCGGGAACCAGAGC CTGGTCAACGAGCTGGCCTTCACCGCCCGCAAGATGATGGCTGACGAGGCCCTGGGCAGT GGGCTGGTCAGCCGGGTGTTCCCAGACAAAGAGGTCATGCTGGATGCTGCCTTAGCGCTG GCGGCCGAGATTTCCAGCAAGAGCCCCGTGGCGGTGCAGAGCACCAAGGTCAACCTGCTG TATTCCCGCGACCATTCGGTGGCCGAGAGCCTCAACTACGTGGCGTCCTGGAACATGAGC ATGCTGCAGACCCAAGACCTCGTGAAGTCGGTCCAGGCCACGACTGAGAACAAGGAACTG AAAACCGTCACCTTCTCCAAGCTCTGA Clone variation with respect to NM_001398.2 122 a=>c >OriGene 5' read for NM_001398 unedited
NGGGTCGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGGCGATG GCGGCGGGGATAGTGGCTTCTCGCAGACTCCGCGACCTACTGACCCGGCGACTGACAGGC TCCAACTACCCGGGACTCAGTATTAGCCTTCGCCTCACTGGCTCCTCTGCACAAGAGGCG GCTTCCGGAGTAGCCCTCGGTGAAGCCCCAGACCACAGCTATGAGTCCCTTCGTGTGACG TCTGCGCAGAAACATGTTCTGCATGTCCAGCTCAACCGGCCCAACAAGAGGAATGCCATG AACAAGGTCTTCTGGAGAGAGATGGTAGAGTGCTTCAACAAGATTTCGAGAGACGCTGAC TGTCGGGCGGTGGTGATCTCTGGTGCAGGAAAAATGTTCACTGCAGGTATTGACCTGATG GACATGGCTTCGGACATCCTGCAGCCCAAAGGAGATGATGTGGCCCGGATCAGCTGGTAC CTCCGTGACATCATCACTCGATACCAGGAGACCTTCAACGTCATCGAGAGGTGCCCCAAG CCCGTGATTGCTGCCGTCCATGGGGGCTGCATTGGCGGAGGTGTGGACCTTGTCACCGCC TGTGACATCCGGTACTGTGCCCAGGATGCTTTCTTCCAGGTGAAGGAGGTGGACGTGGGT TTGGCTGCCGATGTAGGAACACTGCAGCGCCTGCCCAAGTCATCGGGAACCAGAGCCTGG TCAACGAGCTGGCCTTCACCGNCCGCAAGATGATGGCTGACGAGGCCCTGNGCAGTGGGC TGGTCAGCCCGGTGTTCCCAGACAAAGAGTCATGCTGGATGCTGCCTTANCGCTGGCGGN CGAGATTTCAGCCAGAGCCCGGGGCGGTGCAGAGCACCAGGTCACCTGCTGTATC >OriGene 3' read for NM_001398 unedited
CCGCCGCCGCTATCTAGAGTCGAGTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC TTTACTTTGCTTTATTGTTCGGGGGGGGAAAATAAGGCCTTGGGCTTGAAAAACTCATTG TCATAAAGTTATAAACTGGGAAACTGGGTCAAAAGGCATAAAAACAACTGTCATCGCCCA TCCTCCCTTTCTGGGGATGAGGCGGGACAAGGCCGGCCCCCTGGCTGGGGCCTGGGACCC GAGGGCTCTCAAAGCTTGGAAAAGGGGACGGTTTTCAGTTCCTTGTTCTCAGTCGGGGCC TGAACCGACTTCACAAGGTCTTGGGTCTGCAGCATGCTCATGTTCCAGGACGCCACGTAT TTGAGGCTCTCGGCCACCGAATGGTCGCGGGAATACACCAGGTTGACCTTGGTGCTCTGC ACCGCCACGGGGCTCTTGCTGGAAATCTCGGCCGCCAGCGCTAAGGCAGCATCCAACATG ACCTTTTTGGTTGGGAAACCCGGTTGACAGCCCACTGCCCAGGCCCTGTTAGCCATAATT TTGCGGCCGGGAAAGCCACTTGTTGACAACCTCTGTTCCCAAGACCTGGGCAAGCCCCGC ATGTTCCACCTCAGCACCAAACCCCCTCCACCTCTTTACCCGAAAAAACCTCCTGGCCAA CACCGGATTCCCAGCGGTGACAAGCCCACCCTCCCCCAAGCCCCCCCCACGGACGGACCA TCACGGGCCTGGGGCACCTTTCATACCTCTCAAAGCTCCCTGTTTAAGAGTCAATGACCG GGATGCCCCTCGTCCCGGCCCAACTTTCCTTTGCCTCCAAATGCCAAAACACTCCTAAGG AAACCCCCCGAAATTTTTTCTCCCACAAACACCCCCCCCCCACT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001398 |
| Insert Size | 1290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001398.2, NP_001389.2 |
| RefSeq Size | 1276 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 1891 |
| UniProt ID | Q13011 |
| Domains | ECH |
| Gene Summary | This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205622 | ECH1 (Myc-DDK-tagged)-Human enoyl CoA hydratase 1, peroxisomal (ECH1) |
CNY 2400.00 |
|
| RC205622L3 | Lenti ORF clone of Human enoyl CoA hydratase 1, peroxisomal (ECH1), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205622L4 | Lenti ORF clone of Human enoyl CoA hydratase 1, peroxisomal (ECH1), mGFP tagged |
CNY 5890.00 |
|
| RG205622 | ECH1 (tGFP-tagged) - Human enoyl CoA hydratase 1, peroxisomal (ECH1) |
CNY 4000.00 |
