AlaRS (AARS) (NM_001605) Human Untagged Clone
CAT＃: SC119146
AARS (untagged)-Human alanyl-tRNA synthetase (AARS)
CNY 7096.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AARS; CMT2N; DEE29; EIEE29 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119146 sequence for NM_001605 edited (data generated by NextGen Sequencing)
ATGGACTCTACTCTAACAGCAAGTGAAATCCGGCAGCGATTTATAGATTTCTTCAAGAGG AACGAGCATACGTATGTTCACTCGTCTGCCACCATCCCATTGGATGACCCCACTTTGCTC TTTGCCAATGCAGGCATGAACCAGTTTAAACCCATTTTCCTGAACACAATTGACCCATCT CACCCCATGGCAAAGCTGAGCAGAGCTGCCAATACCCAGAAGTGCATCCGGGCTGGGGGC AAACATAATGACCTGGACGATGTGGGCAAGGATGTCTATCATCACACCTTCTTCGAGATG CTGGGCTCTTGGTCTTTTGGAGATTACTTTAAGGAATTGGCATGTAAGATGGCTCTGGAA CTCCTCACCCAAGAGTTTGGCATTCCCATTGAAAGACTTTATGTTACTTACTTTGGCGGG GATGAAGCAGCTGGCTTAGAAGCAGATCTGGAATGCAAACAGATCTGGCAAAATTTGGGG CTGGATGACACCAAAATCCTCCCAGGCAACATGAAGGATAACTTCTGGGAGATGGGTGAC ACGGGCCCCTGTGGTCCTTGCAGTGAGATCCACTACGACCGGATTGGTGGTCGGGACGCC GCACATCTTGTCAACCAGGACGACCCTAATGTGCTGGAGATCTGGAACCTTGTGTTCATC CAGTATAACAGGGAAGCTGATGGCATTCTGAAACCTCTTCCCAAGAAAAGCATTGACACA GGGATGGGCCTGGAACGACTGGTATCTGTGCTGCAGAATAAGATGTCCAACTATGACACT GACCTTTTTGTCCCTTACTTTGAAGCCATTCAGAAGGGCACAGGTGCCCGACCATACACT GGGAAAGTTGGTGCTGAGGATGCCGATGGGATTGACATGGCCTACCGGGTGCTGGCTGAC CACGCTCGGACCATCACTGTGGCACTGGCTGATGGTGGCCGGCCTGACAACACAGGGCGT GGATATGTGTTGAGACGGATTCTCCGCCGAGCTGTCCGATACGCCCATGAAAAGCTCAAT GCCAGCAGGGGCTTCTTTGCTACGTTAGTGGATGTTGTCGTCCAGTCCCTGGGAGATGCA TTTCCTGAGCTGAAGAAGGACCCAGACATGGTGAAGGACATCATTAATGAAGAAGAGGTG CAGTTTCTCAAGACTCTCAGCAGAGGGCGTCGCATCCTGGACAGGAAAATTCAGAGCCTG GGAGACAGCAAGACCATTCCCGGAGACACTGCTTGGCTCCTCTATGACACCTATGGGTTT CCAGTGGATCTGACTGGACTGATTGCTGAAGAGAAGGGCCTGGTGGTAGACATGGATGGC TTTGAAGAGGAGAGGAAACTGGCCCAGCTGAAATCACAGGGCAAGGGAGCTGGTGGGGAA GACCTCATTATGCTGGACATTTACGCTATCGAAGAGCTCCGGGCACGGGGTCTGGAGGTC ACAGATGATTCCCCAAAGTACAATTACCATTTGGACTCCAGTGGTAGCTATGTATTTGAG AACACAGTGGCTACGGTGATGGCTCTGCGCAGGGAGAAGATGTTCGTGGAAGAGGTGTCC ACAGGCCAGGAGTGTGGAGTGGTGCTGGACAAGACCTGTTTCTATGCTGAGCAAGGAGGC CAGATCTATGACGAAGGCTACCTGGTGAAGGTGGATGACAGCAGTGAAGATAAAACAGAG TTTACAGTGAAGAATGCTCAGGTCCGAGGAGGGTATGTGCTACACATTGGAACCATCTAC GGTGACCTGAAAGTGGGGGATCAGGTCTGGCTGTTTATTGATGAGCCCCGACGAAGACCC ATCATGAGCAACCACACAGCTACGCACATTCTGAACTTCGCCCTGCGCTCAGTGCTTGGG GAAGCTGACCAGAAAGGCTCATTGGTTGCTCCTGACCGCCTCAGATTTGACTTTACTGCC AAGGGAGCCATGTCCACCCAACAGATCAAGAAGGCTGAAGAGATTGCTAATGAGATGATT GAGGCAGCCAAGGCCGTCTATACCCAGGATTGCCCCCTGGCAGCAGCGAAAGCCATCCAG GGCCTACGGGCTGTGTTTGATGAGACCTATCCTGACCCTGTGCGAGTCGTCTCCATTGGG GTCCCGGTGTCCGAGTTGCTGGATGACCCCTCTGGGCCTGCTGGCTCCCTGACTTCTGTT GAGTTCTGTGGGGGAACGCACCTGCGGAACTCGAGTCATGCAGGAGCTTTTGTGATCGTG ACGGAAGAAGCCATTGCCAAGGGTATCCGGAGGATTGTGGCTGTCACAGGTGCCGAGGCC CAGAAGGCCCTCAGGAAAGCAGAGAGCTTGAAGAAATGTCTCTCTGTCATGGAAGCCAAA GTGAAGGCTCAGACTGCTCCAAACAAGGATGTGCAGAGGGAGATCGCTGACCTTGGAGAG GCCCTGGCCACTGCAGTCATCCCCCAGTGGCAGAAGGATGAATTGCGGGAGACTCTCAAA TCCCTAAAGAAGGTCATGGATGACTTGGACCGAGCCAGCAAAGCCGATGTCCAGAAACGA GTGTTAGAGAAGACGAAGCAGTTCATCGACAGCAACCCCAACCAGCCTCTTGTCATCCTG GAGATGGAGAGCGGCGCCTCAGCCAAGGCCCTGAATGAAGCCTTGAAGCTCTTCAAGATG CACTCCCCTCAGACTTCTGCCATGCTCTTCACGGTGGACAATGAGGCTGGCAAGATCACG TGCCTGTGTCAAGTCCCCCAGAATGCAGCCAATCGGGGCTTAAAAGCCAGCGAGTGGGTG CAGCAGGTGTCAGGCTTGATGGACGGTAAAGGTGGTGGCAAGGATGTGTCTGCACAGGCC ACAGGCAAGAACGTTGGCTGCCTGCAGGAGGCGCTGCAGCTGGCCACTTCCTTCGCCCAG CTGCGCCTCGGGGATGTAAAGAACTGA Clone variation with respect to NM_001605.2 2715 t=>c >OriGene 5' read for NM_001605 unedited
NGGAACGGTTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGTA CAGCTGCGCGTCTGCGGGAATAGGTGCAGCGGGCCCTTGGCGGGGGACTCTGAGGGAGGA GCTGGGGACGGCGACCCTAGGAGAGTTCTTTGGGGTGACTTTCAAGATGGACTCTACTCT AACAGCAAGTGAAATCCGGCAGCGATTTATAGATTTCTTCAAGAGGAACGAGCATACGTA TGTTCACTCGTCTGCCACCATCCCATTGGATGACCCCACTTTGCTCTTTGCCAATGCAGG CATGAACCAGTTTAAACCCATTTTCCTGAACACAATTGACCCATCTCACCCCATGGCAAA GCTGAGCAGAGCTGCCAATACCCAGAAGTGCATCCGGGCTGGGGGCAAACATAATGACCT GGACGATGTGGGCAAGGATGTCTATCATCACACCTTCTTCGAGATGCTGGGCTCTTGGTC TTTTGGAGATTACTTTAAGGAATTGGCATGTAAGATGGCTCTGGAACTCCTCACCCAAGA GTTTGGCATTCCCATTGAAAGACTTTATGTTACTTACTTTGGCGGGGATGAAGCAGCTGG CTTAGAAGCAGATCTGGAATGCAAACAGATCTGGCAAAATTTGGGGCTGGATGACACCAA AATCCTCCCAGGCAACATGAAGGATAACTTCTGGGAGATGGGTGACACGGGCCCCTGTGG TCCTTGCAGTGAGATCCACTACGACCGGATTGGTGGTCGGGACGCCGCACATCTTGTCAA CCAGNACGACCCTAATGTGCTGGAGATCTGGAACNNCTGTGTCATCCAGTATAACAGGGA AGCTGATGGCATTCTGAAACCTCTTCCCAANAAAGCATTGC >OriGene 3' read for NM_001605 unedited
GTCCGCGGCCGCAATCTANAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTAAAGAATGGCCCGGGGCCCTTTTATTCTTTGGAG AAAAAACGATTCCTACTTGCTGACCCGGAAAGCTCAGGTCTGGAAAGCCGTTTTTTATAA ATGCGAGCGGGACAATCAACAGCAATGCGGGGTTAGGGGTTTTAAACCGAGGGCCCTGAC GGGGAGGGCTCAGGGCAAAAATTTAAGGGGCCCTGAAACATAGGACTGCTCCCAAGGGGG TTCCAATTACTGGGGGGTTAGGAGGGGCTTTTTAAAGGGCCCAAAATTCAAATGTTCTTG TAACAAATAAAAAACTTTTGGCTGGACGGATGGATCCAGGGGGACCCTCCTCCTTCCCCA CTCAGTTTTTTACATCCCCCAGGCGCACTTGGGCAAAGGAAGTGGCCATCTGCAACGCCT CCTGCAGGCAGCCAACGTTTTTTGCCTGGGCCTGTGCAAACCCATCCTTGGCCACCCCTT TTACCGTCCATCAAGCCTGACCCCTGTTGGCCCCCCTCGTTGGGTTTTAAACCCCCGATT GGGTGTTTTTGGGGGCATCCGACCCGGCCCCTGAATTTTCCCCCCTTATTGCCCACCTGA AAAAACCTGCAATACCCCGGGGGGATCCCTTCTGGACAGCCTCCAGGCTTTCTTCGGCCC CTCTGTTTCGGCCCCCTTTTCTCCTTCCATACAAACCTAACCCTGTCGCGGGCCCCCCTA AACAACCCTTTCCCCTCTCCTAACCCCTTCCCCCGCCCTTCCGCCTCTTGCATCGTTCCC ACTCCCCTTTTTTCCCCCCAGCCCCCCATACTTTCCCCCCCCCTCCCTCCCCCCATTCTT TCACTCCTNCCTCCCCGCTCCTCACCGATCTTACCCCTTATTCACCCCCGCCCAGACTAT TTTACTATCCCTTTTCTTACCCCCTTTCCGCTCTACCACTCCCCCAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001605 |
| Insert Size | 3550 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001605.1, NP_001596.1 |
| RefSeq Size | 3344 bp |
| RefSeq ORF | 2907 bp |
| Locus ID | 16 |
| UniProt ID | P49588 |
| Domains | tRNA-synt_2c, DHHA1 |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| Gene Summary | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202136 | AARS (Myc-DDK-tagged)-Human alanyl-tRNA synthetase (AARS) |
CNY 7088.00 |
|
| RC202136L1 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC202136L2 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged |
CNY 9030.00 |
|
| RC202136L3 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), Myc-DDK-tagged |
CNY 9488.00 |
|
| RC202136L4 | Lenti ORF clone of Human alanyl-tRNA synthetase (AARS), mGFP tagged |
CNY 9488.00 |
|
| RG202136 | AARS (tGFP-tagged) - Human alanyl-tRNA synthetase (AARS) |
CNY 8688.00 |
