CPT1A (NM_001876) Human Untagged Clone
CAT＃: SC119000
CPT1A (untagged)-Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 8504.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CPT1; CPT1-L; L-CPT1 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119000 sequence for NM_001876 edited (data generated by NextGen Sequencing)
ATGGCAGAAGCTCACCAAGCTGTGGCCTTTCAGTTCACGGTCACTCCGGACGGGATTGAC CTGCGGCTGAGCCATGAAGCTCTTAGACAAATCTATCTCTCTGGACTTCATTCCTGGAAA AAGAAGTTCATCAGATTCAAGAACGGCATCATCACTGGCGTGTACCCGGCAAGCCCCTCC AGTTGGCTTATCGTGGTGGTGGGCGTGATGACAACGATGTACGCCAAGATCGACCCCTCG TTAGGAATAATTGCAAAAATCAATCGGACTCTGGAAACGGCCAACTGCATGTCCAGCCAG ACGAAGAACGTGGTCAGCGGCGTGCTGTTTGGCACCGGCCTGTGGGTGGCCCTCATCGTC ACCATGCGCTACTCCCTGAAAGTGCTGCTCTCCTACCACGGGTGGATGTTCACTGAGCAC GGCAAGATGAGTCGTGCCACCAAGATCTGGATGGGTATGGTCAAGATCTTTTCAGGCCGA AAACCCATGTTGTACAGCTTCCAGACATCGCTGCCTCGCCTGCCGGTCCCGGCTGTCAAA GACACTGTGAACAGGTATCTACAGTCGGTGAGGCCTCTTATGAAGGAAGAAGACTTCAAA CGGATGACAGCACTTGCTCAAGATTTTGCTGTCGGTCTTGGACCAAGATTACAGTGGTAT TTGAAGTTAAAATCCTGGTGGGCTACAAATTACGTGAGCGACTGGTGGGAGGAGTACATC TACCTCCGAGGACGAGGGCCGCTCATGGTGAACAGCAACTATTATGCCATGGATCTGCTG TATATCCTTCCAACTCACATTCAGGCAGCAAGAGCCGGCAACGCCATCCATGCCATCCTG CTTTACAGGCGCAAACTGGACCGGGAGGAAATCAAACCAATTCGTCTTTTGGGATCCACG ATTCCACTCTGCTCCGCTCAGTGGGAGCGGATGTTTAATACTTCCCGGATCCCAGGAGAG GAGACAGACACCATCCAGCACATGAGAGACAGCAAGCACATCGTCGTGTACCATCGAGGA CGCTACTTCAAGGTCTGGCTCTACCATGATGGGCGGCTGCTGAAGCCCCGGGAGATGGAG CAGCAGATGCAGAGGATCCTGGACAATACCTCGGAGCCTCAGCCCGGGGAGGCCAGGCTG GCAGCCCTCACCGCAGGAGACAGAGTTCCCTGGGCCAGGTGTCGTCAGGCCTATTTTGGA CGTGGGAAAAATAAGCAGTCTCTTGATGCTGTGGAGAAAGCAGCGTTCTTCGTGACGTTA GATGAAACTGAAGAAGGATACAGAAGTGAAGACCCGGATACGTCAATGGACAGCTACGCC AAATCTCTACTACACGGCCGATGTTACGACAGGTGGTTTGACAAGTCGTTCACGTTTGTT GTCTTCAAAAACGGGAAGATGGGCCTCAACGCTGAACACTCCTGGGCAGATGCGCCGATC GTGGCCCACCTTTGGGAGTACGTCATGTCCATTGACAGCCTCCAGCTGGGCTATGCGGAG GATGGGCACTGCAAAGGCGACATCAATCCGAACATTCCGTACCCCACCAGGCTGCAGTGG GACATCCCGGGGGAATGTCAAGAGGTTATAGAGACCTCCCTGAACACCGCAAATCTTCTG GCAAACGACGTGGATTTCCATTCCTTCCCATTCGTAGCCTTTGGTAAAGGAATCATCAAG AAATGTCGCACGAGCCCAGACGCCTTTGTGCAGCTGGCCCTCCAGCTGGCGCACTACAAG GACATGGGCAAGTTTTGCCTCACATACGAGGCCTCCATGACCCGGCTCTTCCGAGAGGGG AGGACGGAGACCGTGCGCTCCTGCACCACTGAGTCATGCGACTTCGTGCGGGCCATGGTG GACCCGGCCCAGACGGTGGAACAGAGGCTGAAGTTGTTCAAGTTGGCGTCTGAGAAGCAT CAGCATATGTATCGCCTCGCCATGACCGGCTCTGGGATCGATCGTCACCTCTTCTGCCTT TACGTGGTGTCTAAATATCTCGCTGTGGAGTCCCCTTTCCTTAAGGAAGTTTTATCTGAG CCTTGGAGATTATCAACAAGCCAGACCCCTCAGCAGCAAGTGGAGCTGTTTGACTTGGAG AATAACCCAGAGTACGTGTCCAGCGGAGGGGGCTTTGGACCGGTTGCTGATGACGGCTAT GGTGTGTCGTACATCCTTGTGGGAGAGAACCTCATCAATTTCCACATTTCTTCCAAGTTC TCTTGCCCTGAGACGGATTCTCATCGCTTTGGAAGGCACCTGAAAGAAGCAATGACTGAC ATCATCACTTTGTTTGGTCTCAGTTCTAATTCCAAAAAGTAA Clone variation with respect to NM_001876.3 >OriGene 5' read for NM_001876 unedited
ACCCCGCCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA TTCGGCACGAGGCCGGCGTCGGGTGCGCTCGGCCTCGCCCGCGGCCCTCCTTCCCCGGCT CCCGCTCGCCGCTCGTTCACTCCACCGCCGCCGCCGCCGCCGCCGCTGCCGCTGCCGCTG CCGCACCTCCGTAGCTGACTCGGTACTCTCTGAAGATGGCAGAAGCTCACCAAGCTGTGG CCTTTCAGTTCACGGTCACTCCGGACGGGATTGACCTGCGGCTGAGCCATGAAGCTCTTA GACAAATCTATCTCTCTGGACTTCATTCCTGGAAAAAGAAGTTCATCAGATTCAAGAACG GCATCATCACTGGCGTGTACCCGGCAAGCCCCTCCAGTTGGCTTATCGTGGTGGTGGGCG TGATGACAACGATGTACGCCAAGATCGACCCCTCGTTAGGAATAATTGCAAAAATCAATC GGACTCTGGAAACGGCCAACTGCATGTCCAGCCAGACGAAGAACGTGGTCAGCGGCGTGC TGTTTGGCACCGGCCTGTGGGTGGCCCTCATCGTCACCATGCGCTACTCCCTGAAAGTGC TGCTCTCCTACCACGGGTGGATGTTCACTGAGCACGGNCAGATGAGTCGTGCCACCAAGA TCTGGATGGGTATGGTCAAGATCTTTTCANGCCGAANACCCATGTTGTACAGCTTCCAGA CATCGCTGCCTCGCCTGCCGGTCCCGGCTGTCAAGACACTGTGAACAGGTATCTACAGTC GGTGAAGCCCTCTATGAAGGAAGAAAGACTCANACGGATGACAGCACTTGCTCAGAATTN TGCTGTCGTCTTGGACCAGATACAGTGTATTTGAGTTAAATCCTGTGGCTACAATACTGA GCACTGTGGGAGG >OriGene 3' read for NM_001876 unedited
CGCCTACTAGTGTACGTTCCGCNCTCNANGATCNATTTTTTTTTTTTTTTTTTGTCTTTT GTTTTCATTTTTTCTTGCTTTCAGGGAGAAAAAAAATCTTTGGCCCATAAAGGTGGGATT TTTACATCATGAAGTATACATACATAAAACATGCATGTGGGCCGCGCATGGTGGCTCATG CCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTCG AGACCAGCCTGACCAATATGATGAAACCCCGTCTCTACTAAAAATACAAAAATCAGCAGG GCATGGTGTTGGGCGTGTAATCCCAGCCACTCGGGAGGCTGAGACAGGAGAATCGCTTGA ACCTGCAAGGCAGATGCTGCAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGGGCAA CAAGAGCGAAACTCCATCTCAAAAAAAAAAAAAAAAAAAAAAGGCACGTGTTCTCCGGCA AGCACCTTCCATCGACTCCAACAGTTCAGCCATCGCTGTTGTACTATCGGGGTGCAGCAC TCTGAAGGAGTCAGGCCCAACTAGAACCAAGTGGTCAAAAAAATAAATACACAATTTAAA ACAATGGACAGCTAAGCTTGTCATGACAGACAGTGGGAATTTCATTCCATGATGCTTCAT TTTTGCATTAAATACTGAAGATGACTCAGTGCATAATTGCTTTGTGGNTCATGAGACGCC TTCGTGAAGGCACTCCTATCTGAAGGACAGGCTGGGTGAACACCTAGCACCGTCACTTGA GACATCTTGGCTGGTCACCAAGTGAGCCCGGCTACTGTCTCCATCACCCTCTGGTGAGGT CTTCCCCTCACCCGGTGGGAAGAGAAAGCCATCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001876 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001876.2, NP_001867.2 |
| RefSeq Size | 2941 bp |
| RefSeq ORF | 2322 bp |
| Locus ID | 1374 |
| UniProt ID | P50416 |
| Domains | Carn_acyltransf |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway |
| Gene Summary | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221740 | CPT1A (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8488.00 |
|
| RC221740L1 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 10888.00 |
|
| RC221740L2 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10888.00 |
|
| RC221740L3 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 7700.00 |
|
| RC221740L4 | Lenti ORF clone of Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 7700.00 |
|
| RG221740 | CPT1A (tGFP-tagged) - Human carnitine palmitoyltransferase 1A (liver) (CPT1A), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 10088.00 |
