GSPT1 (NM_002094) Human Untagged Clone
CAT＃: SC118827
GSPT1 (untagged)-Human G1 to S phase transition 1 (GSPT1), transcript variant 1
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 551G9.2; eRF3a; ETF3A; GST1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC118827 representing NM_002094.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAACTTTCAGAACCTATTGTAGAAAATGGAGAGACAGAAATGTCTCCAGAAGAATCATGGGAGCAC AAAGAAGAAATAAGTGAAGCAGAGCCAGGGGGTGGTTCCTTGGGAGATGGAAGGCCGCCAGAGGAAAGT GCCCATGAAATGATGGAGGAGGAAGAGGAAATCCCAAAACCTAAGTCTGTGGTTGCACCGCCAGGTGCT CCTAAGAAAGAGCATGTAAATGTAGTATTCATTGGGCACGTAGATGCTGGCAAGTCAACCATTGGAGGA CAAATAATGTATTTGACTGGAATGGTTGACAAAAGGACGCTTGAAAAGTATGAAAGAGAAGCTAAAGAG AAAAACAGAGAAACTTGGTACTTGTCTTGGGCCTTAGACACAAATCAGGAAGAACGAGACAAGGGTAAA ACAGTAGAAGTGGGTCGTGCCTATTTTGAAACCGAAAAGAAGCATTTCACAATTCTAGATGCCCCTGGC CACAAGAGTTTTGTCCCAAATATGATTGGTGGTGCCTCTCAAGCTGATTTGGCTGTGCTGGTAATCTCA GCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCA AAGACAGCAGGTGTAAAACACCTAATTGTGCTAATTAATAAGATGGATGATCCAACAGTAAATTGGAGC AATGAGAGATATGAAGAATGTAAGGAGAAACTAGTGCCATTTTTGAAAAAAGTTGGCTTCAATCCCAAA AAGGACATTCACTTTATGCCCTGCTCAGGACTTACTGGAGCAAATCTCAAAGAGCAGTCGGATTTCTGT CCTTGGTACATTGGATTACCGTTTATTCCATATCTGGATAATTTGCCGAACTTCAATAGATCAGTTGAT GGACCAATCAGGCTGCCAATTGTGGATAAGTACAAGGATATGGGCACTGTGGTCCTGGGAAAGCTGGAA TCAGGATCTATTTGTAAAGGCCAGCAGCTTGTGATGATGCCAAACAAGCACAACGTGGAAGTTCTTGGA ATACTTTCCGATGATGTAGAGACTGATACCGTAGCCCCAGGTGAAAACCTCAAAATCAGACTGAAAGGA ATTGAAGAAGAGGAGATTCTTCCAGGGTTTATACTTTGTGATCCTAATAATCTTTGTCATTCTGGACGC ACATTTGATGCCCAGATAGTGATTATAGAGCACAAATCCATCATCTGCCCAGGCTATAATGCGGTGCTG CATATTCATACCTGTATTGAGGAGGTGGAAATAACAGCCTTAATCTGCTTGGTAGACAAAAAATCAGGA GAAAAAAGTAAGACCCGACCCCGTTTTGTGAAACAAGATCAAGTATGCATTGCTCGCTTAAGGACAGCA GGAACCATCTGCCTTGAGACCTTTAAAGACTTCCCTCAGATGGGTCGTTTCACCTTAAGAGATGAGGGT AAGACCATTGCAATTGGAAAAGTTCTGAAACTGGTTCCAGAGAAAGACTAA >OriGene 5' read for NM_002094 unedited
GATGTCAGAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGAGTGT GGAGTCGGGACTGGAGCTGCTGCCGCGGCGACGCCGGGGATCTTTGTCGCTGGCTCCCGG CCCTTCTGCCCCGCCGCCTTCCCTCAGTCAGCGTTGCCCACTCCTCTCCGGCCGGGCGCC CCTGCCTCCATTTCTCGCTCTCTGTCCACCACACACACGGCCCCCCCGATCATGGATCCG GGCAGTGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGAGCAGCAGCGGCAGCAGCAGC AGCGACTCGGCGCCTGACTGCTGGGACCAGGCGGACATGGAAGCCCCCGGGCCGGGCCCT TGCGGCGGCGGCGGCTCCCTGGCGGCGGCGGCCGAGGCCCAGCGGGAGAACCTCAGCGCG GCCTTCAGCCGGCAACTCAACGTCAACGCCAAGCCCTTCGTGCCCAACGTCCACGCCGCC GAGTTCGTGCCGTCCTTCCTGCGGGGCCCGGCAGCGCCGCCACCCCCAGCTGGCGGCGCC GCCAATAACCACGGAGCCGGCAGCGGCGCGGGAGGCCGTGCGGCACCTGTGGAATCCTCT CAAGAGGAACAGTCATTGTGTGAAGGTTCAAATTCAGCTGTTAGCATGGAACTTTCAGAA CCTATTGTAGAAAATGGAGAGACAGAAATGTCTCCAGAAGAATCATGGGAGCACAAAGAA GAAATAAGTGAAGCAGAGCCAGGGGGTGGTTCCTTGGGAGATGGAAAGCCGCCAGAGGAA AGTGCCCATNGAATGATGGNNAGAGAAAGAGGAATCCCCAAACCTAAGTCTGTGGTTGCA CCGCCAGGTGCTCCTAAGAAAGACATGTAAATGTANTATCCATTGGGCACGTAGATGCTG GCAAGTCAACA >OriGene 3' read for NM_002094 unedited
GCGCCACGCATTCTAGAGTCGAGTTTTTCTTTTTTTTTTTTCAAATTGAATATTTTATTA ACATGGTAGTTGCCTTTGCCACATGTGCACACACACTCGCACACTCAAAATGATCTGCCT GGGGGAAAAAATACTAAATATGCCTAAGGGGAAAATGAAAAATAAAAAAATTCCTGTAGG TTTTCATTATTGTAGGCAATTATGTCCACATCACTTACAAAGCTATTGCCAAATCTGTCC AAGGAAGCAGAGTTTGAAGTGAGGGCTAGGGACAGGAATCTTGGGAAAAATTCAACAGTG GCATAGCAGAGCTCTCAATATGAAAAAGCTGACATAATGTGGACTTTTGCTGTGAATTTC CTCTTTGCAAAATATGGGGAGAGGTTTATCAATGGGCAGAAAATAAGAGAAGGCGGTGTG AAGTAGGCTTCTGCAGTCAATTTTCCTCACAGTATTGTGCAGGGTCATCAAGAAAATGCT TAGTCTTTCTCTGGAACCAGTTTCAGAACTTTTCCAATTGCAATGGTCTTACCCTCATCT CTTAAGGTGAAACGACCCATCTGAGGGAAGTCTTTAAAGGTCTCAAGGCAGATGGTTCCT GCTGTCCTTAAGCGAGCAATGCATACTTGATCTTGTTTCACAAAACGGGGTCGGGACTTA CTTTTTTCTACTGATTTTTTGTCTACCAAGCAGATTAAGGCTGTTATTTCCCACTCCTCA ATACAGGTATGAATATGCAGCACCGCATTATAGCCTGGGCAGATGATGGATTTGTGCTCT ATATTACTATCTGGCATCAAAAGCGCGCCAGATGACAAAATTATAGGTACACAAGTTTAA CCCTGGAAGATCCCCTCTCTCATCTCTTTAGCCGATTTGAAGTTCACCTGGCCTAGGTAT AGCCTTAATATCGGAAAATTCAAGACTCCCAGTGGGTTGTTGGATAACACACTGTTGGCT TCAAAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002094 |
| Insert Size | 1500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002094.1 |
| RefSeq Size | 2587 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 2935 |
| Domains | GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2 |
| Protein Families | Transmembrane |
| MW | 55.8 kDa |
| Gene Summary | Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth (PubMed:2511002). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229318 | GSPT1 (Myc-DDK-tagged)-Human G1 to S phase transition 1 (GSPT1), transcript variant 1 |
CNY 4744.00 |
|
| RC229318L1 | Lenti ORF clone of Human G1 to S phase transition 1 (GSPT1), transcript variant 1, Myc-DDK-tagged |
CNY 7144.00 |
|
| RC229318L2 | Lenti ORF clone of Human G1 to S phase transition 1 (GSPT1), transcript variant 1, mGFP tagged |
CNY 7144.00 |
|
| RC229318L3 | Lenti ORF clone of Human G1 to S phase transition 1 (GSPT1), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC229318L4 | Lenti ORF clone of Human G1 to S phase transition 1 (GSPT1), transcript variant 1, mGFP tagged |
CNY 7144.00 |
|
| RG229318 | GSPT1 (tGFP-tagged) - Human G1 to S phase transition 1 (GSPT1), transcript variant 1 |
CNY 6344.00 |
|
| SC320847 | GSPT1 (untagged)-Human G1 to S phase transition 1 (GSPT1), transcript variant 1 |
CNY 3656.00 |
|
| SC327953 | GSPT1 (untagged)-Human G1 to S phase transition 1 (GSPT1) transcript variant 1 |
CNY 5224.00 |
