MGAT1 (NM_002406) Human Untagged Clone
CAT＃: SC118672
MGAT1 (untagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2
CNY 3656.00
| Cited in 1 publication. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I; GnTI; MGAT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118672 sequence for NM_002406 edited (data generated by NextGen Sequencing)
ATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTGTGGGGCGCTATCCTCTTTGTGGCCTGG AATGCCCTGCTGCTCCTCTTCTTCTGGACGCGCCCAGCACCTGGCAGGCCACCCTCAGTC AGCGCTCTCGATGGCGACCCCGCCAGCCTCACCCGGGAAGTGATTCGCCTGGCCCAAGAC GCCGAGGTGGAGCTGGAGCGGCAGCGTGGGCTGCTGCAGCAGATCGGGGATGCCCTGTCG AGCCAGCGGGGGAGGGTGCCCACCGCGGCCCCTCCCGCCCAGCCGCGTGTGCCTGTGACC CCCGCGCCGGCGGTGATTCCCATCCTGGTCATCGCCTGTGACCGCAGCACTGTTCGGCGC TGCCTGGACAAGCTGCTGCATTATCGGCCCTCGGCTGAGCTCTTCCCCATCATCGTTAGC CAGGACTGCGGGCACGAGGAGACGGCCCAGGCCATCGCCTCCTACGGCAGCGCGGTCACG CACATCCGGCAGCCCGACCTGAGCAGCATTGCGGTGCCGCCGGACCACCGCAAGTTCCAG GGCTACTACAAGATCGCGCGCCACTACCGCTGGGCGCTGGGCCAGGTCTTCCGGCAGTTT CGCTTCCCCGCGGCCGTGGTGGTGGAGGATGACCTGGAGGTGGCCCCGGACTTCTTCGAG TACTTTCAGGCCACCTATCCGCTGCTGAAGGCCGACCCCTCCCTGTGGTGCGTCTCGGCC TGGAATGACAACGGCAAGGAGCAGATGGTGGACGCCAGCAGGCCTGAGCTGCTCTACCGC ACCGACTTTTTCCCTGGCCTGGGCTGGCTGCTGTTGGCCGAGCTCTGGGCTGAGCTGGAG CCCAAGTGGCCAAAGGCCTTCTGGGACGACTGGATGCGGCGGCCGGAGCAGCGGCAGGGG CGGGCCTGCATACGCCCTGAGATCTCAAGAACGATGACCTTTGGCCGCAAGGGTGTGAGC CACGGGCAGTTCTTTGACCAGCACCTCAAGTTTATCAAGCTGAACCAGCAGTTTGTGCAC TTCACCCAGCTGGACCTGTCTTACCTGCAGCGGGAGGCCTATGACCGAGATTTCCTCGCC CGCGTCTACGGTGCTCCCCAGCTGCAGGTGGAGAAAGTGAGGACCAATGACCGGAAGGAG CTGGGGGAGGTGCGGGTGCAGTATACGGGCAGGGACAGCTTCAAGGCTTTCGCCAAGGCT CTGGGTGTCATGGATGACCTTAAGTCGGGGGTTCCGAGAGCTGGCTACCGGGGTATTGTC ACCTTCCAGTTCCGGGGCCGCCGTGTCCACCTGGCGCCCCCACCGACGTGGGAGGGCTAT GATCCTAGCTGGAATTAG Clone variation with respect to NM_002406.3 668 g=>a;1304 t=>c >OriGene 5' read for NM_002406 unedited
NNTTTGTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCAGC TCAGCCATGGACTTTGGACCTGGGTAAGAAGAGAGAAGCTGCACTTTGGTGGATCTGATT TGGGATTCTCAGTTTTGAAAATGGAGTGCTGAATGGGGATTTGATGATCTCCTGGAGAGC AACTGAGACAAGAGAAGAAAGGTGCATGGCTGCCTCCTAATCCCATAGTCCAGAGGAGGC ATCCCTAGGACTGCGGGCAAGGGAGCCGGGCAAGCCCAGGGCAGCCTTGAACCGTCCCCT GGCCTGCCCTCCCCGGTGGGGGCCAGGATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTG TGGGGCGCTATCCTCTTTGTGGCCTGGAATGCCCTGCTGCTCCTCTTCTTCTGGACGCGC CCAGCACCTGGCAGGCCACCCTCAGTCAGCGCTCTCGATGGCGACCCCGCCAGCCTCACC CGGGAAGTGATTCGCCTGGCCCAAGACGCCGAGGTGGAGCTGGAGCGGCAGCGTGGGCTG CTGCAGCAGATCGGGGATGCCCTGTCGAGCCAGCGGGGNGAGGGTGCCCACCGCGGCCCC TCCCGCCCAGCCGCGTGTGCCTGTGACCCCCGCGCCGGCGGTGATTCCCATCCTGGTCAT CGCCTGTGACCGCAGCACTGTTCGGCGCTGCCTGGACAAGCTGCTGCATTATCGGCCCTC GGCTGAGCTCTTCCCATCATCGTTAGCCAGGACTGCGGGCACGAGGAGACGGCCCAGGCC ATCGCCTTCTACGGNAGACGCGGTCACGCACATTCGGCAGCCCGACCTGAGACATTTTTG CGGTGCCGNCGGACCACCCGCAGTTCCAGGGCTACTACAGGATCGCGCGCCTTACGCTGG GGCGCTGGGCCAGCTTTTNCCGTAG >OriGene 3' read for NM_002406 unedited
GGGGGGGGANNNNNNNNCCTCTTTTNNNNNNNNNCCTCTGTGNACCTGGACCGCATTTTA NGATCNGTTTTTTTTTTTTTTTTTTACAAACATTCAGCCTTTTATTTTAAAAAAAATTCT GTAGCTTCCACTTTCTTTCATGAAACTGAGGTCAGGCAAGAAACAAAAATCCACCAAGTC CTCTCCATCCTGCCATGGCGTCCTGGCCTGTGAGGACATGGGGCGCCTGGGAGCGGGCGG GGAGGCTGGGCAGCACTGGGCCAGAGGCGTCCTGGTCACTGCTCCACCTGGTCACTGCTC CACCTCATGCTGAGAGGAGCCTGTGTGTCAAACCCCAGGGGAAAAAGGGACAGGCAGATC GAATTCTGTCTTCTACCAAGCCAGCAGGAGCCTCCTAGAGCAACAGGGAGGGCACTGATT TTCAGAAAGGAGGGGTCAGGGCATAGTCCCTCTCTGAGGTAAGGCAAGAGAGAGGTTGTC CCAGCACAAGGCCTTCCTCCCTGCACCTCTCTCCTGACAGCTGGGCATGGGCTGAGGAGA GGTCTTGCTTGCCCCCTTCAACTTTCCATCTCAGAACTATAAACTGCTAGGCTGCAAGGA GAGAGGGCTAAGTGGGGGTCAAACAGGANAGAAGGGCAGGAGGCANTGAGCCCCGATGAC CCACCAACTNCACCNAGCCCTNGACAGGAAGCCCCTTTGGTTAGTATCATTTTGGCCACA AATATGTCCCCCTTCTCCCATAACTCAGTCCCCACTGGNGGAAAGGGGAGCANGTATANA AGGAGGGCTCGTGCCCCTGNNCTGAAAAACCAGGTCAGGNAAAAGGGGTCTGGTCAAGAA AGCGAACGGTGCCAAACTCTCTGCCCATGCTCCAAGAGAAAGCTCAAGACGTGGGCAATT TCTGGCCCCCACAAGCNCCAGGGCCCCCCACCCAACAGTGGGTTTCCTGTCTAATACCCC CGCACAATACCTAAAATAAGTCCCCCTGAACTGNACTCCCTTGAGAAACGGAGAAATATN CCCTCTGNTATCATTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002406 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002406.2, NP_002397.1 |
| RefSeq Size | 2937 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 4245 |
| UniProt ID | P26572 |
| Domains | GNT-I |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 6. Variants 1-23 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and experimental data. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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N-Linked Glycosylation-Dependent and -Independent Mechanisms Regulating CTRP12 Cleavage, Secretion, and Stability
,null,
Biochemistry
,PubMed ID 30566828
[MGAT1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200717 | MGAT1 (Myc-DDK-tagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2 |
CNY 3656.00 |
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| RC200717L1 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2, Myc-DDK-tagged |
CNY 6056.00 |
|
| RC200717L2 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RC200717L3 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
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| RC200717L4 | Lenti ORF clone of Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG200717 | MGAT1 (tGFP-tagged) - Human mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1), transcript variant 2 |
CNY 5256.00 |
