IF 2(Mt) (MTIF2) (NM_002453) Human Untagged Clone
CAT＃: SC118637
MTIF2 (untagged)-Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 5336.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118637 sequence for NM_002453 edited (data generated by NextGen Sequencing)
ATGAACCAGAAGCTACTGAAGTTGGAGAACTTGCTACGATTTCACACTATTTATAGGCAA CTGCACAGTCTGTGTCAAAGAAGAGCATTAAGACAGTGGAGGCATGGGTTTTCATCTGCT TACCCTGTGTGGACAGCTCAACTGTGTGCCTGGCCCTGGCCAACAGATGTGCTCAATGGG GCTGCTTTATCTCAGTATAGGCTTCTAGTAACAAAAAAGGAAGAAGGACCATGGAAATCT CAGTTATCTTCAACAAAATCTAAAAAGGTGGTAGAAGTATGGATTGGAATGACTATTGAG GAACTGGCCAGGGCAATGGAAAAAAACACAGATTATGTATATGAAGCTTTATTGAACACT GATATTGACATAGATTCACTGGAAGCAGACTCACATTTAGATGAAGTCTGGATCAAAGAA GTGATAACGAAGGCAGGGATGAAGTTAAAGTGGAGTAAATTAAAACAGGACAAAGTCAGA AAAAATAAAGATGCTGTAAGAAGGCCCCAGGCAGATCCAGCTTTATTAACCCCAAGGTCC CCAGTTGTTACTATAATGGGCCATGTTGATCACGGGAAAACGACATTACTTGACAAATTT CGAAAAACTCAAGTGGCAGCAGTGGAAACTGGAGGCATCACTCAGCACATTGGTGCCTTT CTTGTCTCTCTGCCTTCTGGGGAAAAGATAACTTTTCTTGATACTCCAGGACATGCTGCT TTCTCAGCAATGAGAGCCAGAGGTGCTCAGGTCACTGACATTGTCGTATTGGTTGTAGCT GCAGATGATGGAGTGATGAAACAAACTGTAGAATCTATTCAGCATGCCAAAGATGCACAG GTTCCTATTATCCTTGCCGTAAATAAATGTGACAAAGCTGAGGCTGATCCTGAGAAAGTG AAAAAAGAGCTGCTGGCTTACGATGTGGTATGTGAAGATTATGGAGGTGATGTTCAAGCA GTGCCTGTCTCCGCACTTACGGGCGATAATCTGATGGCTTTGGCAGAAGCAACAGTTGCT CTTGCAGAAATGTTAGAATTGAAAGCAGATCCCAATGGTCCAGTGGAAGGAACAGTAATA GAGTCTTTCACAGACAAAGGAAGAGGTCTTGTTACTACAGCTATAATTCAAAGAGGAACT TTAAGAAAAGGCTCTGTTCTGGTTGCTGGAAAATGTTGGGCAAAAGTACGCTTAATGTTT GATGAAAATGGAAAAACAATTGATGAGGCCTATCCCAGCATGCCAGTGGGAATTACAGGC TGGAGAGACCTTCCTTCTGCAGGAGAAGAAATTCTTGAAGTAGAATCTGAGCCAAGGGCA CGTGAAGTTGTTGACTGGAGGAAATATGAACAAGAACAGGAGAAAGGTCAGGAGGATCTG AAAATAATAGAAGAAAAGCGAAAGGAACACAAAGAAGCACATCAGAAAGCCCGTGAGAAG TATGGCCATCTACTGTGGAAGAAGAGATCAATTCTACGGTTTTTAGAAAGAAAAGAACAA ATACCCTTAAAGCCAAAAGAGAAAAGGGAAAGAGATTCAAATGTACTTTCTGTGATTATT AAAGGTGATGTTGATGGTTCTGTTGAGGCCATTTTGAACATTATAGATACCTATGATGCT TCACACGAGTGTGAACTAGAATTAGTACATTTTGGAGTGGGTGATATAAGTGCAAATGAT GTTAACCTTGCTGAAACATTTGATGGTGTTATATATGGCTTTAATGTGAATGCAGGCAAT GTTATCCAACAGTCAGCTGCAAAAAAAGGAGTAAAAATTAAACTTCACAAAATAATTTAC CGTCTTGTTGAAGATTTGCAAGAGGAACTGAGCAGCAGATTACCCTGTGCTGTGGAAGAG CACCCAGTAGGTGAGGCATCTATACTAGCTACCTTCTCTGTAACAGAAGGGAAGAAAAAA GTTCCTGTGGCTGGCTGCAGAGTCCAAAAGGGACAGTTAGAAAAACAAAAAAAATTTAAA CTAACCCGTAATGGACATGTAATTTGGAAGGGCTCATTAACCTCATTGAAACACCATAAA GATGACATTTCAATTGTCAAAACGGGAATGGATTGTGGTCTCAGTTTAGATGAAGACAAT ATGGAATTTCAAGTGGGAGACAGAATTGTTTGTTATGAAGAAAAGCAAATTCAAGCCAAG ACTTCTTGGGATCCAGGATTTTAA Clone variation with respect to NM_002453.2 176 c=>a;1666 g=>a >OriGene 5' read for NM_002453 unedited
TTTATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCTCCGCCCGTGCT CAGAATCCAGGGGCCCGGGGCTGTAGATTCCTTGACAAGGATATCCTAGCGGCGAAACAA CACCGTACTGGGAGTCAGAACGTCTGGGTTCTAGTCTTGACTGCCATTAACTAGCGAGAT CCGGAAAATGAGGCCCATAGGAAACAAGTGACTTGCTGAGTCCAGATAACACTGACTGTC AGAGAGAAACATGAACCAGAAGCTACTGAAGTTGGAGAACTTGCTACGATTTCACACTAT TTATAGGCAACTGCACAGTCTGTGTCAAAGAAGAGCATTAAGACAGTGGAGGCATGGGTT TTCATCTGCTTACCCTGTGTGGACAGCTCAACTGTGTGCCTGGCCCTGGCCAACAGATGT GCTCAATGGGGCTGCTTTATCTCAGTATAGGCTTCTAGTAACAAAAAAGGAAGAAGGACC ATGGAAATCTCAGTTATCTTCAACAAAATCTAAAAAGGTGGTAGAAGTATGGATTGGAAT GACTATTGAGGAACTGGCCAGGGCAATGGAAAAAAACACAGATTATGTATATGAAGCTTT ATTGAACACTGATATTGACATAGATTCACTGGAAGCAGACTCACATTTAGATGAAGTCTG GATCAAAGAAGTGATAACGAAGGCAGGGATGAAGTTAAAGTGGAGTAAATTAAAACAGGA CANAGTCAGAAAAAATAAAGATGCTGTAAGAAAGCCCCCAGCAGATCCAGCTNTATTAAA CCCCAAGTCCCCAGTTGTTACTATAATGGGGCCATGTGATCACGGGAAACGAATTACTTG ACCAATTCGAAAACTCAGTGGCAGCAGTGGAAACTGGAGGCTCACTCACACATTGGGCCT TTCTGNCTCTCTGCTCCTGGGAAAGATACTT >OriGene 3' read for NM_002453 unedited
ACCCGCACCCCCCCCCGCCGCCCCNGGGGCCGAACACCNCNNCCTTTTGACTCTGCACCG CGGNCCGCATACTAGGATCGNGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTGGGGTATTTACATTTTTAATGGAATTTTAAAAACCTGGATCCCAAA AAGTCTTGGCTTGAATTTGCTTTTTTTCATAACAAACAATTTTGTCTCCCACTTGAAATT CCATATTGGCTTCATTTAAACTGAAACCACAATCCATTCCCGTTTTGACAATTGAAATGG CATTTTTATGGGGTTTCAATGAGGGTAATGAGCCCTTCCAAATTACATGGCCATTACGGG TTAATTTAAAATTTTTTTGGTTTTCTAACTGGCCCTTTTGGACTTTGCAGCCAGCCACAG GAACTTTTTTTTTCCCTTTTGTTACAAAAAAGGGAGCTAGTATAAATGCCTCACCTACTG GGGGCTCTTCCACAACACAGGGGAATCTGGTGGTCAATTCCTTTTGCAAATCTTTAACAA AACGGGAAATTATTTTGGGAAGGTAAAATTTTACTCCTTTTTTTGCAGCTGACTGGTGGA TAACATTGCCTGCATTCACATTAAAGCCCTATATAACACCCTCAAATGTTTCAACAAGGG TAACATCATTTGCACTTATATCACCCACTCCAAAATGGCCTAATTCTAATTCACACTCGG GGGAAGCATCATAGGTATCTATAATGTTCAAAATGGCCTTAACAGAACCATCAACATTCC CTTTATAATCCCCGAAAGGCCCTTTGAAACTCTTTCCCCTTTCTTTTTTGGGCTTTAAGG AATTTGGTCTTTTTCTTTTAAAAACCGGAAAAATGATCTCCCTCCCCAAGAAAATGCCCC TAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002453 |
| Insert Size | 2710 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002453.2, NP_002444.2 |
| RefSeq Size | 2544 bp |
| RefSeq ORF | 2184 bp |
| Locus ID | 4528 |
| UniProt ID | P46199 |
| Domains | GTP_EFTU, GTP_EFTU_D2 |
| Gene Summary | During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. [provided by RefSeq, Mar 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219573 | MTIF2 (Myc-DDK-tagged)-Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5320.00 |
|
| RC219573L3 | Lenti ORF clone of Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 7700.00 |
|
| RC219573L4 | Lenti ORF clone of Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 7700.00 |
|
| RG219573 | MTIF2 (tGFP-tagged) - Human mitochondrial translational initiation factor 2 (MTIF2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6928.00 |
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