GPNMB (NM_002510) Human Untagged Clone
CAT＃: SC118567
GPNMB (untagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2
CNY 4176.00
CNY 9030.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HGFIN; NMB; PLCA3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002510, the custom clone sequence may differ by one or more nucleotides
ATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGATGCCGCCAAAC GATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAGCACAATCAATTAAATGGCTG GTCTTCTGATGAAAATGACTGGAATGAAAAACTCTACCCAGTGTGGAAGCGGGGAGACATGAGGTGGAAA AACTCCTGGAAGGGAGGCCGTGTGCAGGCGGTCCTGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATA TAACATTTGCGGTGAACCTGATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGA GAAGAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATGGTCAGAG GACAGTGACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAACCTTTTCCTC ACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTCAGTATTTCCAGAAATT GGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAATGTGACACTTGGGCCTCAACTCATGGAAGTG ACTGTCTACAGAAGACATGGACGGGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGGTAACAG ATCAGATTCCTGTGTTTGTGACTATGTTCCAGAAGAACGATCGAAATTCATCCGACGAAACCTTCCTCAA AGATCTCCCCATTATGTTTGATGTCCTGATTCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAAC TACAAGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTTCCACCAATCATACTGTGAATCACACGTATG TGCTCAATGGAACCTTCAGCCTTAACCTCACTGTGAAAGCTGCAGCACCAGGACCTTGTCCGCCACCGCC ACCACCACCCAGACCTTCAAAACCCACCCCTTCTTTAGGACCTGCTGGTGACAACCCCCTGGAGCTGAGT AGGATTCCTGATGAAAACTGCCAGATTAACAGATATGGCCACTTTCAAGCCACCATCACAATTGTAGAGG GAATCTTAGAGGTTAACATCATCCAGATGACAGACGTCCTGATGCCGGTGCCATGGCCTGAAAGCTCCCT AATAGACTTTGTCGTGACCTGCCAAGGGAGCATTCCCACGGAGGTCTGTACCATCATTTCTGACCCCACC TGCGAGATCACCCAGAACACAGTCTGCAGCCCTGTGGATGTGGATGAGATGTGTCTGCTGACTGTGAGAC GAACCTTCAATGGGTCTGGGACGTACTGTGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCAC GAGCACCCTGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAACAGTGCCCTGATC TCCGTTGGCTGCTTGGCCATATTTGTCACTGTGATCTCCCTCTTGGTGTACAAAAAACACAAGGAATACA ACCCAATAGAAAATAGTCCTGGGAATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGCAAA AGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGATCCGCTACTCAAAAACCAAGAATTTAAAGGAGTTTCT TAA >OriGene 5' read for NM_002510 unedited
NGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGGACGGG CCCAGAGGAATTCAGAGTTAAACCTTGAGTGCCTGCGTCCGTGAGAATTCAGCATGGAAT GTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGCAAGATTGCCACTTGATGCCGCCA AACGATTTCATGATGTGCTGGGCAATGAAAGACCTTCTGCTTACATGAGGGAGCACAATC AATTAAATGGCTGGTCTTCTGATGAAAATGACTGGAATGAAAAACTCTACCCAGTGTGGA AGCGGGGAGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCGGTCCTGA CCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTGAACCTGATATTCC CTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGTCTATGAGAAGAACTGCAGAAATG AGGCTGGTTTATCTGCTGATCCGTATGTTTACAACTGGACAGCATGGTCAGAGGACAGTG ACGGGGAAAATGGCACCGGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAACCTTTTC CTCACCACCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTCAGT ATTTCCAGAAATTGGGACGATGTTCAGTGAGAGTTTCTGTGAACACAGCCAATGTGACAC TTGNGCCTCAACTCATGGAAGTGACTGTCTACAGAAGACATGGACGGGCATATGTTCCCA TCGCACAAGTGAAAGATGTGTACGTGGTAACAGATCAGATNCCTGTGTTTGTGACTATGT TCCAGAAAGAACGATCGAATTCATCCGACGAAACCTTTCTCAAAGACTCCCCATTATGTT GATGTCCTGATNCAGATCC >OriGene 3' read for NM_002510 unedited
GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCTTTTTTTTTCAGGAGGGAAAA AAAAAAAATGTCTTGTTTAACATTTAGAAAAGGGTTTTATTTCCAAACAGGGGGGAAATC CTTTTTGATAAAGTTGCCTTAAAAAACCAGGGCCCTTTTTTTTGGAAAGTTTTAATTTTT GATTTAAAATTTGGGCCGGGCCTGTCCAAAATTTTGGAAATTTCCCCAAAAGGGCCCCTC ACCTTCTTGCCTGGTTTTTAAAAACCCCTACCTAAGGGCCCTTGGAAAAAAGGAATTTTT GAATTTTTTTCTTTCACTCACCCAAACAAAGTAGGTTGTCCCCAAAATCCTCCCCCCCTT TTTTGAAAGTATTTTTTTTTTTGAGTTTAACAGGGGGGCCCATTTTACCAAAGGTTTGGG CCTGTTTTTGTCCCATCAGGAGTTCATGAGGGGGTTTTGAAGGGGGATTCATTTACCTTG GAACATGATTTTAAAAACGGGCCTACTTTGGTTTTATGCTTCCAGTTCCTTGGAAAGCTG AAAGTTTCCCCCCTTTTTTTGCAGTTAACCGGGGTAAGGCTTTAATCAATAGGAGTTTTT TTCCTTACTTTGGGGACACAGGGTTTTTATCCTACTAGTTCCCTAAAACTTTTTAAAGGG AAACTTAAAAATTATGAAAAAACCCCTGCCTAAGTGCTTTTATAAANTTCCCAACATGCC TGAAGCTGCCTGCAGTTAAATCCCTCTCCCATTCTTTAATGACCTTTACCTTTAAAAATT CCCTTCCCCAACCCAAATTCTTTTAACAATATTTTTAGAAAAAAGGTTATACCCCCTCCA CCATTCCATTATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002510 |
| Insert Size | 2740 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002510.2, NP_002501.1 |
| RefSeq Size | 2739 bp |
| RefSeq ORF | 1683 bp |
| Locus ID | 10457 |
| UniProt ID | Q14956 |
| Domains | PKD |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217699 | GPNMB (Myc-DDK-tagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2 |
CNY 5610.00 |
|
| RC217699L3 | Lenti-ORF clone of GPNMB (Myc-DDK-tagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2 |
CNY 7510.00 |
|
| RC217699L4 | Lenti-ORF clone of GPNMB (mGFP-tagged)-Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2 |
CNY 6984.00 |
|
| RG217699 | GPNMB (tGFP-tagged) - Human glycoprotein (transmembrane) nmb (GPNMB), transcript variant 2 |
CNY 6184.00 |
