RBM4 (NM_002896) Human Untagged Clone
CAT＃: SC118336
RBM4 (untagged)-Human RNA binding motif protein 4 (RBM4), transcript variant 1
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LARK; RBM4A; ZCCHC21; ZCRB3A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118336 sequence for NM_002896 edited (data generated by NextGen Sequencing)
ATGGTGAAGCTGTTCATCGGAAACCTGCCCCGGGAGGCTACAGAGCAGGAGATTCGCTCA CTCTTCGAGCAGTATGGGAAGGTGCTGGAATGTGACATCATTAAGAATTACGGCTTTGTG CACATAGAAGACAAGACGGCAGCTGAGGATGCCATACGCAACCTGCACCATTACAAGCTT CATGGGGTGAACATCAACGTGGAAGCCAGCAAGAATAAGAGCAAAACCTCAACAAAGTTG CATGTGGGCAACATCAGTCCCACCTGCACCAATAAGGAGCTTCGAGCCAAGTTTGAGGAG TATGGTCCGGTCATCGAATGTGACATCGTGAAAGATTATGCCTTCGTACACATGGAGCGG GCAGAGGATGCAGTGGAGGCCATCAGGGGCCTTGATAACACAGAGTTTCAAGGCAAACGA ATGCACGTGCAGTTGTCCACCAGCCGGCTTAGGACTGCGCCCGGGATGGGAGACCAGAGC GGCTGCTATCGGTGCGGGAAAGAGGGGCACTGGTCCAAAGAGTGTCCGATAGATCGTTCA GGCCGCGTGGCAGACTTGACCGAGCAATATAATGAGCAATACGGAGCAGTGCGTACGCCT TACACCATGAGCTATGGGGATTCATTGTATTACAACAACGCGTACGGAGCGCTCGATGCC TACTACAAGCGCTGCCGTGCTGCCCGGTCCTATGAGGCAGTGGCAGCTGCAGCTGCCTCC GTGTATAATTACGCAGAGCAGACCCTGTCCCAGCTGCCACAAGTCCAGAATACAGCCATG GCCAGTCACCTCACCTCCACCTCTCTCGATCCCTACGATAGACACCTGTTGCCGACCTCA GGAGCTGCTGCCACAGCTGCTGCTGCAGCAGCAGCCGCTGCTGCTGTTACTGCAGCTTCC ACTTCATATTACGGGCGGGATCGGAGCCCCCTGCGTCGCGCTACAGCCCCAGTCCCCACT GTTGGAGAGGGCTACGGTTACGGGCATGAGAGTGAGTTGTCCCAAGCTTCAGCAGCCGCG CGGAATTCTCTGTACGACATGGCCCGGTATGAGCGGGAGCAGTATGCCGATCGGGCGCGG TACTCAGCCTTTTAA Clone variation with respect to NM_002896.3 >OriGene 5' read for NM_002896 unedited
NNTTTCNGGATTTTGNTATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGGGAA GCGTCCGCGCCGCGAGGAGGAGGCCCTGCTGGTTTCTGTGCGGGCTCTTGTCAGGATGGT GAAGCTGTTCATCGGAAACCTGCCCCGGGAGGCTACAGAGCAGGAGATTCGCTCACTCTT CGAGCAGTATGGGAAGGTGCTGGAATGTGACATCATTAAGAATTACGGCTTTGTGCACAT AGAAGACAAGACGGCAGCTGAGGATGCCATACGCAACCTGCACCATTACAAGCTTCATGG GGTGAACATCAACGTGGAAGCCAGCAAGAATAAGAGCAAAACCTCAACAAAGTTGCATGT GGGCAACATCAGTCCCACCTGCACCAATAAGGAGCTTCGAGCCAAGTTTGAGGAGTATNG GTCCGGTCATCGAATGTGACATCGTGAAAGATTATGCCTTCGTACACATGGAGCGGGCAG AGGNATGCAGTGGAGGCCATCAGGGGCCTTGATAACACAGAGTTTCAAGGCAAACAATGC CGTGCCAGTTGTCCACCAGCCGGCTTAGGACGCGCCCGGGATAGGGAGACAGGAGCGGCT GTATCGGGTGCGGGAAAAGAGGGGCACTGGCCAAAAGAGTGTCCGATAGGATCGTTCAGG CCGCGTTGGCAGACTTGACCGAGCAATATAATGAGCATAGGGGAGCAAGTGCGTACGCCT TACACCATGACTATGGGGGGATTCCATTGTATTACAACAAACGCGTACGGGAGCGCTTCG ATGCCTACTAAAGGGGCTGGCGTGTTTGCCCGTCCTTTTAAAGGCATTGGGCAGCTGCAG CTGCCCTCCGTGTATAATTACCCCANAGCAGACCCTGTTCCAGCTTGGCACAGTTCCAGA TACAGCCCTGGCCAGTCACCTCACCTCCACTTTTCTCGATACCA >OriGene 3' read for NM_002896 unedited
CGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGGAAAAACCTTTTTTTTCCCCGGAAAAAAA AAAAGGGACCCAACTTTTTAGGGTTAGCCTTTGGACCAAAAAAAAACCCTGGAGCAGGGA AACTTTCACCAAGGGCTTTCCCAAATCCAACACCCCCAGGAGGCAGGAGGCAGGGAGGAA ACAGCACACCCCCCACCACAGTTTTTGCACACAATGGGGCCTGGTGGGAAAAAAAAACAT GAAGGGGAACCTCCAAAAGTTTTGAGGGACAAAAAAACAGGAAAATGGGCCGGAAAGGAA AGGAAAGGAAAGGAAAAAAAGGTCTTAACTTAACAAGGGAAATTAAGGGCCACGGTTCCT GAGGGACTGAACCCACAAAGCCGAAAACGTCCCGGAAATGGGGTACCACAAAGGGGGTAT TCTCATGCACAACCGGAGCTCGGAATTTCAGCCCCACACATCCCACCTCAAGCTTTAAAA GGCTGAGTACCGCGCCCCGATCGGATACTGCTCCCGCTCATACCGGGCCATTGTNGAACA GAGAATTCCCGCGCGGTTGCTGAAAGCTTGGGACAATCTCACCTCTCATGCCCCGTACCC GGTAACCCTTCTTCCAACAGGGGGGGACTGGGGGCTTGTAACCCCGACCCCAGGGGGGCC CTCCATCCCCCCCCGGTAATATGAAGTTGGAACGCTGAAATAAACACAAACCAAGGGTTG CTTGTTGAAACAACAACTGTGGCAACAACTCCTGAGGTCCGCAACAAGGGTTTATCTTAA GGATCCCAAAAAGTGGGGGGNGAGGGGACCGGCCATGGTTTTATTCTTGACTTGGGGCAC CTGGGAAAGGGTTTCTTCTGCNTATATTAACCGGGAGAGGTGGAGGTGCCACTGCCTTAA GAACGGGCAAACCGGAACCTCTGAAAAGCCTCAAGCCCTCCACCCCTTGTTTAAACACGA ACCCCTACTTGGGGTAAGGCGCCCCTGTCCCTTGCATATATTGTCGCAAAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002896 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002896.1, NP_002887.1 |
| RefSeq Size | 1620 bp |
| RefSeq ORF | 1101 bp |
| Locus ID | 5936 |
| UniProt ID | Q9BWF3 |
| Domains | RRM, zf-CCHC |
| Protein Families | Druggable Genome |
| Gene Summary | RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3' UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3' UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207607 | RBM4 (Myc-DDK-tagged)-Human RNA binding motif protein 4 (RBM4), transcript variant 1 |
CNY 3656.00 |
|
| RC207607L3 | Lenti ORF clone of Human RNA binding motif protein 4 (RBM4), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC207607L4 | Lenti ORF clone of Human RNA binding motif protein 4 (RBM4), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG207607 | RBM4 (tGFP-tagged) - Human RNA binding motif protein 4 (RBM4), transcript variant 1 |
CNY 5256.00 |
