TLE2 (NM_003260) Human Untagged Clone
CAT＃: SC118085
TLE2 (untagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1
CNY 5448.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ESG; ESG2; GRG2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_003260, the custom clone sequence may differ by one or more nucleotides
ATGTACCCCCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGA TCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGA GAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTC AACATTGAAATGCATAAGCAGGCGGAGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCT TCCTGACCCAGGAGCATCAGCAGCAGGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGA GCTGAACAGCCTCATCGGGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCC CGCCCAGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCCAGG CTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAGAGC CCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGT GGTGGCGGGAAGCAGAGAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTG ATTACAATCTGGTGGTGGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAA GGTACCCATCTGCATTCCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGC TCACCGCTGCCTAGAGCCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCT GTGACTCCTCCCCGCCCCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGC TGCCAAGCCAGCACCTTCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACC ACGTCCTTCAGCCTGGGCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCC TCCACCTGTCCCCCCAGGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCA TCCCCATCTCCGAGGGTCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCC TTCCACGTGTCTGCGGACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCA TCCCGCGGCACGCCCGGCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGG CTCCACACAGCATGTGTACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCC AAGACGCCCGTGGCCCAGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGG ATGGCCGGAGTCTGATCGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCC CCGTATCAAGGCCGAGCTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAG GTTTGCTTCTCCTGCTGCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGC AGTTCCAGGGCCACACGGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGG GGGCCTGGACAACACGGTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGC TCCCAGATTTTCTCCCTGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACG TGGAGATCCTGCACGTCCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCT GAAGTTTGCCTCCTGCGGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGGACG CCGTACGGGGCCAGCATTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAAATA ACAAATACATCGTGACAGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTACTGA >OriGene 5' read for NM_003260 unedited
AAATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCCGCTTCTCCCTCC TCCCTCTTTCTTCGGGCAGCCTCCCCACCACCCCACTTCAGCCTCCCCCACTCTTGCCGC CTCCATATCATCAAGCTCTGGTGGCGCCTGGGGGGCTTTTCGGATCGGCAGGATGTACCC CCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGA GATCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAA GCTAGAATGTGAGAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTA TTATGAGATGTCGTACGGGCTCAACATTGAAATGCATAAGCAGGCGGACATTGTGAAGCG TCTGAGCGGTATCTGCGCTCAGATTATCCCCTTCCTGACCCAGGAGCATCAGCAGCAGGT GCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGAGCTGAACAGCCTCATCGG GCAGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCCCGCCC AGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGC CCAGGCTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCCGAGGGTC CAAAGTGGAGAGAGCCCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGA GGGAGAGCGACCGAGTGGCCCTNGGTGGTGGCGGNNAGCANAGAGCANATGAGAAGGAGC CATCANGACCTTTATGAAGCGACGAAGACCAGAGTGATA >OriGene 3' read for NM_003260 unedited
TGACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTGATAGATACATTT TATTTCCACCGAGGTCCCCTGCCCATCGGCCCCCACATGCAGCAGGGGAAGGTGTGAAGC CGTTGGCCAGAGAGCAGATGGGATGTACGGTTCCTAGGCAGGGCTGGGAGGCGGCTGCTA GGATGTCTGTCCCGGCTGCTGATTCCCCTGGGAGTCTGGACTTCGGGTACAGGAAGGGGG GTCATGTCTCAGTAGACCACCTCATACACGGTGGCCTTCTTGTCCCCCGAGCCTGTCACG ATGTATTTGTTATTTCTGGAGATGTCACAACTCAGGACTGAGGACGACTCCTTGGACTGG AAAATGCTGGCCCCGTACGGCGTCCTCCAGGCGTTGAGCAGGTTGTCCTTCCCGGTGCTC ACAAACCACCGTCCGCAGGAGGCAAACTTCAGGGACAGCACGCAGCTCTCGTGGAGGTGC AGCTGGTATTTCTCCGGCTTGCGGACGTGCAGGATCTCCACGTTGCTACTCTCCATTCCG ACCGCCAGCCAGTCCTGGTTAGGGCAGTGGCCCAGGGAGAAAATCTGGGAGCTGAAGTCA TGCTGCTGCAGCTGGCGGCCCTCCCGCAGGTCCCAGCAGCGCACCGTGTTGTCCAGGCCC CCTGTCCAGAGCCGAGTGCCGTAATCGGAAATATCAATGCAGCTGGCGCCGTCCGTGTGG CCCTGGAACTGCCTGACCATAGTCTGATTCTGCAGGTCCCCAGACCACATGTTGCCATCG CTGCAGCAGGAGAAAGCAACCTTGGCGTCNGGGCTGACGGCCANGCGTAACAAGCTGGGG CTGANGAANNTCACTCGCTGCGCGNGGGGGGGGGGCCCCCCCAAGTTCCAATGGACACAG GGGCTGTCCTACCGCCCACATCAGACTCCGGCATTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003260 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003260.3, NP_003251.2 |
| RefSeq Size | 2579 bp |
| RefSeq ORF | 2232 bp |
| Locus ID | 7089 |
| UniProt ID | Q04725 |
| Domains | WD40, TLE_N |
| Protein Families | Transcription Factors |
| Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 1, which is shorter by an amino acid, compared to isoform 4. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202474 | TLE2 (Myc-DDK-tagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1 |
CNY 5440.00 |
|
| RC202474L1 | Lenti ORF clone of Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1, Myc-DDK-tagged |
CNY 7840.00 |
|
| RC202474L2 | Lenti ORF clone of Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC202474L3 | Lenti ORF clone of Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202474L4 | Lenti ORF clone of Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG202474 | TLE2 (tGFP-tagged) - Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1 |
CNY 4370.00 |
