TXNRD1 (NM_003330) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Synonyms	GRIM-12; TR; TR1; TRXR1; TXNR
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC118040 sequence for NM_003330 edited (data generated by NextGen Sequencing) ATGTCATGTGAGGACGGTCGGGCCCTGGAAGGAACGCTCTCGGAATTGGCCGCGGAAACC GATCTGCCCGTTGTGTTTGTGAAACAGAGAAAGATAGGCGGCCATGGTCCAACCTTGAAG GCTTATCAGGAGGGCAGACTTCAAAAGCTACTAAAAATGAACGGCCCTGAAGATCTTCCC AAGTCCTATGACTATGACCTTATCATCATTGGAGGTGGCTCAGGAGGTCTGGCAGCTGCT AAGGAGGCAGCCCAATATGGCAAGAAGGTGATGGTCCTGGACTTTGTCACTCCCACCCCT CTTGGAACTAGATGGGGTCTYGGAGGAACATGTGTGAATGTGGGTTGCATACCTAAAAAA CTGATGCATCAAGCAGCTTTGTTAGGACAAGCCCTGCAAGACTCTCGAAATTATGGATGG AAAGTCGAGGAGACAGTTAAGCATGATTGGGACAGAATGATAGAAGCTGTACAGAATCAC ATTGGCTCTTTGAATTGGGGCTACCGAGTAGCTCTGCGGGAGAAAAAAGTCGTCTATGAG AATGCTTATGGGCAATTTATTGGTCCTCACAGGATTAAGGCAACAAATAATAAAGGCAAA GAAAAAATTTATTCAGCAGAGAGATTTCTCATTGCCACTGGTGAAAGACCACGTTACTTG GGCATCCCTGGTGACAAAGAATACTGCATCAGCAGTGATGATCTTTTCTCCTTGCCTTAC TGCCCGGGTAAGACCCTGGTTGTTGGAGCATCCTATGTCGCTTTGGAGTGCGCTGGATTT CTTGCTGGTATTGGTTTAGACGTCACTGTTATGGTTAGGTCCATTCTTCTTAGAGGATTT GACCAGGACATGGCCAACAAAATTGGTGAACACATGGAAGAACATGGCATCAAGTTTATA AGACAGTTCGTACCAATTAAAGTTGAACAAATTGAAGCAGGGACACCAGGCCGACTCAGA GTAGTAGCTCAGTCCACCAATAGTGAGGAAATCATTGAAGGAGAATATAATACGGTGATG CTGGCAATAGGAAGAGATGCTTGCACAAGAAAAATTGGCTTAGAAACCGTAGGGGTGAAG ATAAATGAAAAGACTGGAAAAATACCTGTCACAGATGAAGAACAGACCAATGTGCCTTAC ATCTATGCCATTGGCGATATATTGGAGGATAAGGTGGAGCTCACCCCAGTTGCAATCCAG GCAGGAAGATTGCTGGCTCAGAGGCTCTATGCAGGTTCCACTGTCAAGTGTGACTATGAA AATGTTCCAACCACTGTATTTACTCCTTTGGAATATGGTGCTTGTGGCCTTTCTGAGGAG AAAGCTGTGGAGAAGTTTGGGGAAGAAAATATTGAGGTTTACCATAGTTACTTTTGGCCA TTGGAATGGACGATTCCGTCAAGAGATAACAACAAATGTTATGCAAAAATAATCTGTAAT ACTAAAGACAATGAACGTGTTGTGGGCTTTCACGTACTGGGTCCAAATGCTGGAGAAGTT ACACAAGGCTTTGCAGCTGCGCTCAAATGTGGACTGACCAAAAAGCAGCTGGACAGCACA ATTGGAATCCACCCTGTCTGTGCAGAGGTATTCACAACATTGTCTGTGACCAAGCGCTCT GGGGCAAGCATCCTCCAGGCTGGCTGCTGAGGTTAA Clone variation with respect to NM_003330.2 321 c=>y 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_003330 unedited GGGGGGGGAACTTTTTTNCCCCCCCGTTAATTGTATCGATCATTAGGCGCCGGATTCGCC GAGTGAAGGAGGCNCTGAGTCTTCATCATTCTCAAATTCTTGTAAGCTCTGCGTCGGGTG AAACCAGACAAAGCCGCGAGCCCAGGGATGGGAGCACGCGGGGGACGGCCTGCCGGCGGG GACGACAGCATTGCGCCTGGGTGCAGCAGTGTGCGTCTCGGGGAAGGGAAGATATTTTAA GGCGTGTCTGAGCAGACGGGGAGGCTTTTCCAAACCCAGGCAGCTTCGTGGCGTGTGCGG TTTCGACCCGGTCACACAAAGCTTCAGCATGTCATGTGAGGACGGTCGGGCCCTGGAAGG AACGCTCTCGGAATTGGCCGCGGAAACCGATCTGCCCGTTGTGTTTGTGAAACAGAGAAA GATAGGCGGCCATGGTCCAACCTTGAAGGCTTATCAGGAGGGCAGACTTCAAAAGCTACT AAAAATGAACGGCCCTGAAGATCTTCCCAAGTCCTATGACTATGACCTTATCATCATTGG AGGTGGCTCANGAGGTCTGGCAGCTGCTAAGGAGGCAGCCCAATATGGCAAGAAGGTGAT GGTCCTGGACTTTGTCACTCCCACCCCTCTTGGAACTAGATGGGGTCTTGGAGGAACATG TGTGAATGTGGGTTGCATACCTAAAAACTGATGCATCAAGCAGCTTTGTTAGGACAAGCC CTGCAAGACTCTCGAAAATATGGATTGAAAGTCCAAGAGACAGTTAAGCCTGATTGGGAC ACATGATAGAAACTGTCCAGATCACATTGGCTTTTGAATTGGGCTACCGAGTACCTCTGG GGGAGAAAAAGCCGCCTAGGAAAAGCTTATGGGCAATTAATTGGTCCTCCCAGGATTAGG GCACCAATATTAAGGCCAAGAAAAATTTATTCCCCCAAGATTCTCATTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_003330 unedited GGCCTTTAATTTAAGTTTCACATTGTTTTGTTTTTTTTCAGAAGATTGTAACAAAAATAC ATGGNAACTTAGTATATGTTTCAATAAAATGGGTAAATACAGAATCTGAACAAGACAAGT CTATAAGATATAATCCTTCTTGTGGGGATAGAACATTCCACTCCACTAAGAAAAGAGAAT CACAACATGATTAGGCCTGATTATCTCATACCGCCTACTGTTCTAATTTCAGAGACTGGT GGACTTGGAGAAAAAAACAAATTAAAAATAAATAAATAAAACTGAATATGGTCAACTGCC TCAATTGCTCTCTCCTTTTCCCTTTTCCCATTTCTTTACACAGCCAAATGAGATGAGGAC GTGAGGCAGAGCCCTGTGGGGGACGGTCAGGGGCTCTGCTGCCTAAATGCCAGGCAGATG CATGGGTTCCCTGAGGTGGCCCAGAAATGTATTTTGACCACTCAGTGCTGCAACTGAGAA ATACAGGTAGAGTAGCAGGAATGAAAAAGAAGCTTCAAGAACTGCTGGCAGGAGGCCTGA TGTCCACAAGCACGTGTTCAAAACTCAACCTACACAGTTAGAAAAGACAGCTGAGATAGA ATCCTCAGCCAGTACATTGACAAACACATAACTGAGGCATTAATACCTCTTTATAATAAT GCAAGTTGAAATGCTAACAAAGCATAAACACTTCTGCAAAAATTCCACAAAGCACAGTTG TTCATTCAACAGAAAAAGTCAAAACCACTTGGTTNTTAAATGATAATCCTTCACATCCNA CCTGNTGTNCAAAATATTAATNNATAACTCATGATATTAATAACCTNCCTTTNAAAAAGC ATTACCACAAACAGCTAAATCATAAGCATGAAAAGACTTATNTTCCTACTAATGTAGTNN CTANAAAGAATAGATACCCCATGTCATTATTTTTTTAGCATAAGCTAAGCTACAGAAAAA CTTTAACAGTTACGTGTTTTCATGCGAATGATCCACCTGCTAGACACGCACATAACTAN
Restriction Sites	NotI-NotI
ACCN	NM_003330
Insert Size	4420 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003330.2, NP_003321.3
RefSeq Size	3923 bp
Locus ID	7296
UniProt ID	Q16881
Domains	pyr_redox, pyr_redox_dim
Protein Families	Druggable Genome
Protein Pathways	Pyrimidine metabolism
Gene Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. [provided by RefSeq, May 2017] Transcript Variant: This variant (4) uses an alternate donor splice site at the 5' terminal exon, which results in translation initiation from an in-frame upstream start codon compared to variant 1. The encoded isoform (2, also known as TXNRD1_v2) has a longer and distinct N-terminus, and is thought to differentially regulate genes associated with differentiation and adhesion compared to isoform 1 (PMID:26464515).

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