EIF3S1 (EIF3J) (NM_003758) Human Untagged Clone
CAT＃: SC117778
EIF3J (untagged)-Human eukaryotic translation initiation factor 3, subunit J (EIF3J)
CNY 2400.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | eIF3-alpha; eIF3-p35; EIF3S1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117778 sequence for NM_003758 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCGGCGGCGGCGGCGGGGGACTCGGACTCCTGGGACGCCGACGCTTTCTCC GTGGAAGACCCAGTGCGGAAGGTGGGGGGCGGCGGCACTGCCGGCGGGGACCGCTGGGAA GGCGAGGACGAGGACGAGGACGTCAAGGATAACTGGGATGACGATGATGATGAAAAAAAA GAGGAAGCAGAAGTAAAACCAGAGGTAAAAATTTCAGAAAAGAAAAAAATAGCAGAGAAG ATAAAAGAGAAAGAACGGCAACAGAAGAAAAGGCAAGAAGAAATTAAAAAGAGGTTAGAA GAACCCGAAGAACCTAAAGTGCTAACACCAGAAGAACAATTAGCAGATAAACTGCGGCTA AAGAAATTACAGGAAGAGTCAGACCTCGAATTAGCAAAGGAAACTTTTGGTGTTAATAAT GCAGTTTATGGAATAGATGCTATGAACCCATCTTCAAGAGATGACTTTACAGAGTTTGGA AAGTTACTAAAAGATAAAATTACACAATATGAAAAGTCACTATATTATGCCAGTTTTTTG GAAGTCTTAGTTCGAGATGTGTGTATTTCATTGGAAATTGATGACTTGAAAAAAATTACC AATTCACTGACTGTGCTTTGCAGTGAAAAACAGAAGCAAGAAAAGCAAAGCAAAGCCAAA AAGAAGAAGAAAGGTGTGGTTCCTGGAGGGGGATTAAAAGCCACCATGAAAGATGATCTG GCAGATTATGGTGGTTATGATGGAGGATATGTACAAGACTATGAAGACTTCATGTGA Clone variation with respect to NM_003758.2 >OriGene 5' read for NM_003758 unedited
CGCGTCGAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCACCTCC GCCGTGCTAACTCCTCGCTAGCTCTCCCTCTCACACACGCTCACACCCGGCTCGAGATGG CGGCGGCGGCGGCGGCGGCGGGGGACTCGGACTCCTGGGACGCCGACGCTTTCTCCGTGG AAGACCCAGTGCGGAAGGTGGGGGGCGGCGGCACTGCCGGCGGGGACCGCTGGGAAGGCG AGGACGAGGACGAGGACGTCAAGGATAACTGGGATGACGATGATGATGAAAAAAAAGAGG AAGCAGAAGTAAAACCAGAGGTAAAAATTTCAGAAAAGAAAAAAATAGCAGAGAAGATAA AAGAGAAAGAACGGCAACAGAAGAAAAGGCAAGAAGAAATTAAAAAGAGGTTAGAAGAAC CCGAAGAACCTAAAGTGCTAACACCAGAAGAACAATTAGCAGATAAACTGCGGCTAAAGA AATTACAGGAAGAGTCAGACCTCGAATTAGCAAAGGAAACTTTTGGTGTTAATAATGCAG TTTATGGAATAGATGCTATGAACCCATCTTCAAGAGATGACTTTACAGAGTTTGGAAAGT TACTAANAGATAANATTACACAATATGAAAAGTCACTATATTATGCCAGTTNTTTGGAAG TCTTAGTTCGAGATGTGTGTATTTCATTGGAAATTGATGACTTGAAAAAAATACCATTCA CTGACTGTGCTTTGCAGTGAANAACANGAGCAAGAAAAAGCAAGCAAAGCCCAAAAGAAG AAGAAAGGTGTGGTTCCCTGGAGGGGATTAAAAGCCACCATGAAAGATGATCTGGCAGAT TATGGTGGTATGATGGAGGAATTTGTACAAGACTATGGAGACTTCATGTGACATTNNTAT CTTTCTGGNGTCATCCTAATGTTGCCCACATCCCTTGAA >OriGene 3' read for NM_003758 unedited
GAATTTAATTATTATTTACACAGTTAAGGAACAGGTGATACATTTTCATTTGTTAGAAAC TGATCTTTCTGTAATAAAATAGATTTTCAATTCAGTGTATGTCATTATTACTGCTAAGGA AATCTTAGCCCTTGTCTGCCTTAAAGGAATCTTTATTTAATTTACTGTAATTATTGCTGT GTAGTCACTACTTTTGTTAATTTCTCAAATCACTTAGATGATGGTCTTGTTTTCCACTTA GTAGGTATACAGAGCTTTGACGTTCCTATTATTTCCTATATAAGAGAAATTTAAAACATT TTTTGTTCTTTCTGTCTTAGGGGAATAAAAAAACACTAACCACACATTTGGTTAAACTGC TTAGGAGAAGACATAATAAAGATTCCCCAATCTATACTTAACAGCCATAAACCTGAGTTA CAGGCTCAGTTACTCCAAATAAATAGTTCTTATAGGTACTTAATTAATTAGGCCTTGGTA TCTAAATAACAAAATAATGTCCCCAAATAAATAAGAGGAAGGCAGCCCTATACCTTTGTT GCTCTATGGTGACAACATAAGGACCTGGCAAATCACTCATGTTTTAAAGCTGTAACTATG TCAAGCATTTATTAAGATTTAGTAGAGAACGTAGAGAACTGTACTCCCAAGAGCTGTGTT CTATACTCTCAAAAAGTATCTCTTGGGAGGTAACACANAATTTAAGTGCTCACAACAGTG ATTTTATTAAAATTAGTGCTTTATAAAACATTGCAGATGTCATAATTTGTAACATAACAA TTTACCAAACTGTAGTTAACTGGCGCAGCTTGCTGAGCATGTTTTATTCAAGGAAAGGAA ACAAATTGCCAAAACCCTGGTTAAGTTGTTCCATCGCAGCCTAAGAGAACAATGATTTGT CTCTCACACCCTTAATCATGCCCTAAAAATAGTTCCCTCCCCCACTGAAGCGGTCATCCT AGAAATACCTGTTAATACTAGCAGGCTTTGCCCTCAAGATCTCCAACTGTATCCAATATG GAAGTTACTTCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003758 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003758.1, NP_003749.1 |
| RefSeq Size | 2443 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 8669 |
| UniProt ID | O75822 |
| Gene Summary | This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201188 | EIF3J (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit J (EIF3J) |
CNY 2400.00 |
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| RC201188L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit J (EIF3J), Myc-DDK-tagged |
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| RC201188L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit J (EIF3J), mGFP tagged |
CNY 5890.00 |
|
| RG201188 | EIF3J (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit J (EIF3J) |
CNY 4000.00 |
