GALNT4 (NM_003774) Human Untagged Clone
CAT＃: SC117755
GALNT4 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4)
CNY 4312.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GALNAC-T4; GALNACT4 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117755 sequence for NM_003774 edited (data generated by NextGen Sequencing)
ATGGCGGTGAGGTGGACTTGGGCAGGCAAGAGCTGCCTGCTGCTGGCGTTTTTAACAGTG GCCTATATCTTCGTGGAGCTCTTGGTCTCTACTTTTCATGCCTCCGCAGGAGCCGGCCGT GCCAGGGAGCTGGGGTCAAGAAGGCTCTCAGACCTCCAGAAAAATACGGAGGATTTGTCT CGACCGCTTTATAAGAAGCCCCCTGCAGATTCCCGTGCACTTGGGGAGTGGGGGAAAGCC AGCAAACTCCAGCTCAACGAGGATGAACTGAAGCAGCAAGAAGAACTCATTGAGAGATAC GCCATCAATATTTACCTCAGTGACAGGATTTCCCTGCATCGACACATAGAGGATAAAAGA ATGTATGAGTGTAAGTCCCAGAAGTTCAACTATAGGACACTTCCTACCACCTCTGTTATC ATTGCTTTCTATAACGAAGCCTGGTCGACTTTGCTCCGTACCATTCACAGTGTTTTAGAA ACTTCTCCTGCAGTTCTTTTGAAAGAGATCATCTTGGTGGATGACTTGAGTGACAGAGTT TATTTGAAGACACAACTTGAAACTTACATCAGCAATCTTGATAGAGTACGCTTGATTAGG ACCAATAAGCGAGAGGGGCTGGTTAGGGCCCGTCTGATTGGGGCCACTTTCGCCACTGGG GACGTCCTCACTTTCCTGGATTGTCACTGTGAGTGTAATTCCGGTTGGCTGGAACCGCTT TTGGAAAGGATTGGGAGAGATGAAACAGCAGTTGTGTGTCCTGTTATAGACACAATTGAT TGGAATACTTTTGAATTCTATATGCAGATAGGGGAGCCCATGATTGGTGGGTTTGACTGG CGTTTAACATTTCAGTGGCATTCTGTCCCCAAACAGGAAAGGGACAGGCGGATATCAAGA ATTGACCCCATCAGATCACCTACCATGGCTGGAGGACTGTTTGCTGTCAGCAAGAAATAT TTTCAGTACCTTGGAACGTATGACACAGGAATGGAAGTGTGGGGAGGTGAAAACCTTGAG CTGTCTTTTAGGGTGTGGCAGTGTGGTGGCAAATTGGAGATCCACCCGTGTTCCCACGTG GGCCATGTGTTCCCCAAGCGGGCACCATATGCTCGCCCCAATTTCCTACAGAATACTGCT CGGGCAGCAGAAGTTTGGATGGATGAATACAAAGAGCACTTCTACAATAGAAACCCTCCA GCAAGAAAAGAAGCTTATGGTGATATTTCTGAAAGAAAATTACTACGAGAGCGGTTGAGA TGCAAGAGCTTTGACTGGTATTTGAAAAACGTTTTTCCTAATTTACATGTTCCAGAGGAT AGACCAGGCTGGCATGGGGCTATTCGCAGTAGAGGGATCTCGTCTGAATGTTTAGATTAT AATTCTCCTGACAACAACCCCACAGGTGCTAACCTTTCACTGTTTGGATGCCATGGTCAA GGAGGCAATCAATTCTTTGAATATACTTCAAACAAAGAAATAAGGTTTAATTCTGTGACA GAGTTATGTGCAGAGGTACCTGAGCAAAAAAATTATGTGGGAATGCAAAATTGTCCCAAA GATGGGTTCCCTGTACCAGCAAACATTATTTGGCATTTTAAAGAAGATGGAACTATTTTT CACCCACACTCAGGACTGTGTCTTAGTGCTTATCGGACACCGGAGGGCCAACCTGATGTA CAAATGAGAACTTGTGATGCTCTAGATAAAAATCAAATTTGGAGTTTTGAGAAATAG Clone variation with respect to NM_003774.4 1670 g=>a >OriGene 5' read for NM_003774 unedited
TATTTCCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGGGAGAGAGGAGTTGGGCTGTGCCGGAGGCCGAGGACCGAGAGGG CTCAGGTGACCCCTGGAAAGCCTGGGTGGCTGGAAAGGAGCCTAGCGCCTGCATGAAAGG AAGAACCTGCTGGGAAGTACCTGAGCTCGAGCTGTGGGTTCCGCCGCCCTTCCCCTGCGT GGTGGCTTGGTGGCCGCGTCTGCGCCTCAGCCCTGAGAATCCGGATGGCGGTGAGGTGGA CTTGGGCAGGCAAGAGCTGCCTGCTGCTGGCGTTTTTAACAGTGGCCTATATCTTCGTGG AGCTCTTGGTCTCTACTTTTCATGCCTCCGCAGGAGCCGGCCGTGCCAGGGAGCTGGGGT CAAGAAGGCTCTCAGACCTCCAGAAAAATACGGAGGATTTGTCTCGACCGCTTTATAAGA AGCCCCCTGCAGATTCCCGTGCACTTGGGGAGTGGGGGAAAGCCAGCAAACTCCAGCTCA ACGAGGATGAACTGAAGCAGCAAGAAGAACTCATTGAGAGATACGCCATCAATATTTACC TCAGTGACAGGATTTCCCTGCATCGACACATAGAGGATAAAAGAATGTATGAGTGTAAGT CCCAGAAGTTCAACTATAGGACACTTCCTACCACCTCTGTTATCATTGCTTTCTATAACG AAGCCTGGTCGACTTTGCTTCGTACCATTCACAGTGTTTTAGAAACTTCTCCTGCAGTTC TTTTGAAAGAGACATCNTGGTGGATGACTTGAGTGACAGAGNTTATTTGTAGACCAACTT GAAACTTACTCAGCATCTTGATAGAGACGCT >OriGene 3' read for NM_003774 unedited
NCGGTTTCACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTT ATCAGGCACACTTTTTATTTTACATCTTAAACAACATTACAAATACAAAAGGAAAAAAGT CAGAAACAACTCATCACAGAGGAAAAAAACATCATTCAGAAGAGATTAATCTGAACTTCA ACGTTCTTTGTGCTCATTTTAAAATTATTAAAACAGGAATTCAAAAGGACAAGCAAATAA AAACCAAGTATTTTATTAATTAAAATTGAGACCCTAAACCAACTAAGACTATACAACTTA AAAATAAGCTGTCTTCATCCTAGAAAAGTTTGATTTGCCATCTTATAATGAATATGCAGG AACTTACTAATGGGTAAGTAAACAAATTTTCTTTTACAAACAAGTTATTTTGAGTTATAG GGATCCTCCTGGTGACTAGATTTTTTTTAATGACTAAAAATGCCTATTTAGATAGTCAAC CTATCCGTAAAGTTGGACACTAAATGACATAGTGAACATTTAAAGTTGAAACTCTTTGTC TACCAAATATTTTCCTAAAACTGTTTTTAAGCCTATACATTAACTTAAAAGGTCAACTCT TAATTGTCTATAGCCAAAGAGAGCTTTGGTACCCATAAATTCGAATTAAGGAATCGGTTT TTTTAGATGTAGAATTACAGAGTATTTTTGGAATTAAATTGGAACGTGCCTCAATATGAA AATGTGGAAGTCCCACCTTTCTGGTATGTGCCCTATTATGGAGGGATGAACAGCCCTACC ATACTGGATTATTTGAATAAATAAAACACCCCCGGTAAAACTCCTCCTCAAAAAATGCTG GGGGCCCACTCGCCATTTTGGGGCGAAAAGTCCATAAAGGATTAAGGGGCTTTATTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003774 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003774.3, NP_003765.2 |
| RefSeq Size | 5360 bp |
| RefSeq ORF | 1737 bp |
| Locus ID | 8693 |
| UniProt ID | Q8N4A0 |
| Domains | RICIN, Glycos_transf_2 |
| Protein Pathways | Metabolic pathways, O-Glycan biosynthesis |
| Gene Summary | This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts, which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene, also exist. [provided by RefSeq, Dec 2010] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207176 | GALNT4 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4) |
CNY 4304.00 |
|
| RC207176L3 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC207176L4 | Lenti ORF clone of Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4), mGFP tagged |
CNY 6704.00 |
|
| RG207176 | GALNT4 (tGFP-tagged) - Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4) |
CNY 5904.00 |
