Tryptophan rich protein (WRB) (NM_004627) Human Untagged Clone
CAT＃: SC117233
WRB (untagged)-Human tryptophan rich basic protein (WRB), transcript variant 1
CNY 2400.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHD5; WRB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117233 sequence for NM_004627 edited (data generated by NextGen Sequencing)
ATGAGCTCAGCCGCGGCCGACCACTGGGCGTGGTTGCTGGTGCTCAGCTTCGTGTTTGGA TGCAATGTTCTTAGGATCCTCCTCCCGTCCTTCTCATCCTTCATGTCCAGGGTGCTGCAG AAGGACGCGGAGCAGGAGTCACAGATGAGAGCGGAGATCCAGGACATGAAGCAGGAGCTC TCCACAGTCAACATGATGGACGAGTTTGCCAGATATGCCAGGCTGGAAAGAAAGATCAAC AAGATGACGGATAAGCTCAAAACCCATGTGAAAGCTCGGACAGCTCAATTAGCCAAGATA AAATGGGTGATAAGTGTCGCTTTCTACGTATTGCAGGCTGCCCTGATGATCTCACTCATT TGGAAGTATTATTCTGTCCCTGTGGCTGTCGTGCCGAGTAAATGGATAACCCCTCTAGAC CGCCTGGTAGCCTTTCCTACTAGAGTAGCAGGTGGTGTTGGAATTACCTGTTGGATTTTA GTCTGTAACAAAGTTGTCGCTATTGTGCTTCATCCGTTCAGCTGA Clone variation with respect to NM_004627.4 >OriGene 5' read for NM_004627 unedited
TCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGTGGAGCTGCCGT AGCGGACCCAGCACAGCCAGGAGCGTCCGGGATGAGCTCAGCCGCGGCCGACCACTGGGC GTGGTTGCTGGTGCTCAGCTTCGTGTTTGGATGCAATGTTCTTAGGATCCTCCTCCCGTC CTTCTCATCCTTCATGTCCAGGGTGCTGCAGAAGGACGCGGAGCAGGAGTCACAGATGAG AGCGGAGATCCAGGACATGAAGCAGGAGCTCTCCACAGTCAACATGATGGACGAGTTTGC CAGATATGCCAGGCTGGAAAGAAAGATCAACAAGATGACGGATAAGCTCAAAACCCATGT GAAAGCTCGGACAGCTCAATTAGCCAAGATAAAATGGGTGATAAGTGTCGCTTTCTACGT ATTGCAGGCTGCCCTGATGATCTCACTCATTTGGAAGTATTATTCTGTCCCTGTGGCTGT CGTGCCGAGTAAATGGATAACCCCTCTAGACCGCCCTGGTAGCCTTTCCTACTAGAGTAG CAGGTGGTGTTGGAATTACCTGTTGGATTTTAGTCTGTAACAAAGTTGTCGCTATTGTGC TTCATCCGTTCAGCTGAACAGGAGGATGGATACAGCCGCGAGGCCTAAAAACGGATTTCC TCTTCCCTACTTAAAATCTGATTTACACTGTTTTGTTTTTTAAGAAACCCAAGTGCATAG TTTAGATTCTCTTTTTGGTGAAAATGCTTGCTCTTGGACTTTATGAGATAGTCTTTTAAG ATCACGATTTTCTCACCCGTCATGGACCCAGAAGGCCAGTTTATGACCCGTATGTCCTAT GGAACACGTCCTCCATCTGTCCCAAATGCGAAATGCTTAGGAACCTCCCCTGCTGGCCCT TGGGATTTGCCCTCTTAG >OriGene 3' read for NM_004627 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTCAGATTAATTTAATCA GGACTTATAAGGTTTGTTTTCTGTGTTCCATTCTAAAGAGCATAAATATTAAATGCTTAT AAAATACACTTCATTCAAAATGCTTTAAATACAAAACACAAGTTGAGCACAAAACAATGC ATCATGGTCTCGTGGATACATTTACAAAACACTGTAATGAAAAATTAACAGCCTCAAGTA AGTAGATAATACTATACAACTCTTGCTCAACAAATGACATTTTAAACAAAGGGAGAAAAA GCCCACGCAATGTCAATTAGTGTTGTTCTTTTAACCATATTGAATGTGATTAAAGAAGAA ATTTTCGAATTTTTAGTAGCGGTATTAAAATTAAGTTACTTTCTAAACTGGATTAAAAAC AGCACTAAGTCTTCAAAGAACTTACTTAAAGTTGTTAGAAAATTTTGACTTTCCAGTTGG CAATATGACCAAAATACATAAGAGGGCAAATCACAAGTGACAGCATGGAGATGTCCCTAA ACATTTCACATTTCGTACAGATCGAGGACGGTGTTCACTAGTACATACGTGTCATAAACT GGACTTTCTTGGCTCAATGACAGGTGTAGAAAATCGTGATTCTTATAAGACTATCTCATA AAGTCCAAGAACAAACATATTCAACAAAAAAAAAATCTAAACTATGCACTTTTGTTTCTT AAAAAACAAAACAGTGTAAATCAGATTTTAAGCTAGGAAGAGGAAATCCGTTTTTTAGCC TCGCGGCTGTATCCATCCTCNCTGTCAGCTGAACGGATGAAGCACAATAGCGACAACTTT GTTACAGACTANAATCCCACAGGTAATTNNCACACCACCTGCTACTCTAGTANGAAAGGC TACCCAAGCGTCTAAAGGGGTATNCCANTACTCCGNACGAAGCACAGGAACGNATATACT NCAATGAGTGAACATCAGGCAGCTGCATAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004627 |
| Insert Size | 1260 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004627.2, NP_004618.2 |
| RefSeq Size | 1360 bp |
| RefSeq ORF | 525 bp |
| Locus ID | 7485 |
| UniProt ID | O00258 |
| Domains | CHD5 |
| Protein Families | Transmembrane |
| Gene Summary | This gene is located in the candidate region for congenital heart disease (CHD) in Down syndrome (DS). It encodes a basic protein that functions as a receptor that promotes insertion of tail-anchored proteins in the endoplasmic reticulum membrane. This gene is located at a maternally-methylated differentially methylated region (DMR); however, its transcription may be biallelic, not imprinted. Alternative splicing results in different transcript variants. A pseudogene has been defined on chromosome 4. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (1) encodes the longest isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202035 | WRB (Myc-DDK-tagged)-Human tryptophan rich basic protein (WRB), transcript variant 1 |
CNY 2400.00 |
|
| RC202035L1 | Lenti ORF clone of Human tryptophan rich basic protein (WRB), transcript variant 1, Myc-DDK-tagged |
CNY 4800.00 |
|
| RC202035L2 | Lenti ORF clone of Human tryptophan rich basic protein (WRB), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC202035L3 | Lenti ORF clone of Human tryptophan rich basic protein (WRB), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202035L4 | Lenti ORF clone of Human tryptophan rich basic protein (WRB), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG202035 | WRB (tGFP-tagged) - Human tryptophan rich basic protein (WRB), transcript variant 1 |
CNY 4000.00 |
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