VAPB (NM_004738) Human Untagged Clone
CAT＃: SC117187
VAPB (untagged)-Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1
CNY 2400.00
CNY 2950.00
| Cited in 2 publications. |
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALS8; VAMP-B; VAP-B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_004738, the custom clone sequence may differ by one or more nucleotides
ATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGAGCTCAAATTCCGAGGTCCCTTCACCG ATGTTGTCACCACCAACCTAAAGCTTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTAC AGCACCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGCCTCAATTAATGTATCT GTGATGTTACAGCCTTTCGATTATGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGT TTGCTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTATGGATTC AAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAACCACATGATGTAGAAATAAATAAAATT ATATCCACAACTGCATCAAAGACAGAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACA CCGAAGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGAGGCTACGGGAGGAGAA CAAGCAGTTCAAGGAAGAAGATGGACTGCGGATGAGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCA TTAGCCCCAACTGGGAAGGAAGAAGGCCTTAGCACCCGGCTCTTGGCTCTGGTGGTTTTGTTCTTTATCG TTGGTGTAATTATTGGGAAGATTGCCTTGTAG >OriGene 5' read for NM_004738 unedited
GCTTTGCGGATTTGTAATCCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGC GCCCTCGCCCTCCGCCCCTGCGCCTGCACCGCGTAGACCGACCCCCCCCCCCATCGCGCC CACCCGGTAGAGGACCCCCGCCCGTGCCCCGACCGGTCCCCGCCTTTTTGTAAAACTTAA AGCGGGCGCAGCATTAACGCTTCCCGCCCCGGTGACCTCTCATGGGTCTCCCCGCCAAAG GTGCTCCGCCGCTAAGGAACATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGC ACGAGCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCACCAACCTAAAGCTTGGCA ACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTACAGCACCACGTAGGTACTGTG TGAGGCCCAACAGCGGAATCATCGATGCAGGGGCCTCAATTAATGTATCTGTGATGTTAC AGCCTTTCGATTATGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGT TTGCTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACC TTATGGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAACCACATG ATGTAGAAATAAATAAAATTATATCCACAACTGCATCANAGACAGANACACCAATAGTGT CTAAGTCTCTGAGTTCTTCTTTGGATGACACCGAAAGTAAGAAAGTTATGGAAGAATGCT AGATGCTGNCAGGTGAGCTCANATGCTACGGGAGGAGAACAAGCAGTTCAAGGAAAGAGA TGGACTGCNGATGATGAAGACAGTGCAGAGCATCAGCCCCATTGCAGCATTAGCCCCCAC TGGGAAGGAAGAATGCCNNTANCACCCNGCTCTTGGCTCTGGTAGGGTTGGTCCTTATCG TAGGAGTATNTATG >OriGene 3' read for NM_004738 unedited
TTTATTATAATTTTTCTTTTCAGAATGCTACATAGTCAAACAGAACTGGGTTGGTCTTTT ATGGCATAAAATTAATTCACAGTCAAACTCTCAACTAGTACTAGAAGGATTCGCTGAAGA GTTTCTCTCTTCCCTTGCTCCCCACCCACAACTGCTGGTGCCCTCGCTTCTAACCCTCTG CAGCCCGATCCATTTGTCCTGACTCCAGGCTAGGTCCCCAAGGGGAGGTCAGGCTCAGAC TTGGACCTGGGCGCTGGAAGTGTGAGTAATGGTTGAGAGGTGGTATAAGAACAATCTAGA AGACTGACTACTCTTCATTAAAAAAGGTAACCTAGAGTTGACTGTCACTACTGAGAATTT CCCTCCCTATGACAGTTGTTATTTACTGACCCAGAAAAAACACTTCCTGGCAGCTCTTCT CCAGCATCCCAATAAACCCCACCTCCACATGACCAGACTCTCCAGAGACAGAGGAGCCCA CAGCCCTCCAGCAGCAATACCAGTTCACCCAACAGAGTTCAGCAGCACGCAGCATTAACT GTACTCTTGTGACAATAAGAAAACATGAGATGAAATACATTAAATAAGTTAAATATGCAT TAAACATCTCTGAATAACAGTTTGACTTCACTACATGAACCAATACAAATTTAGCTTGGT CCCTTTAAGTGAACAAAGCACTGCCATNTATTTCCAAAGCGCTGGGTCAATCAGTCAACA CACGCCTCTCACTTATGACGAGTACGTCTGATGCTGNCACAGTGCAATTCCTTTTGGCTT CTGACTCTGNTCATCAGTCCCAAGGAGCTGATGGCTTGGACAGAAACTGAGACAGGNAAA GCAGNCTGGCCCGGNAGTGGGCCACGTNNGGGACTACTGTGTGNAGCTGACCGNNACTCC CACATGCTGGGNCTCAGCTCCCCAGGGCCAGCACCACAGGCGAGGAGGGTTTCATTNTAG AGAGGTATCCAGCTCTGTACCAGGCCTACATGNTAAATGACNTACATTTGCATGNGGCAT ACTAAGGGTTCCCCTGCATATAAGACTA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004738 |
| Insert Size | 2440 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004738.3, NP_004729.1 |
| RefSeq Size | 3689 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 9217 |
| UniProt ID | O95292 |
| Domains | MSP_domain |
| Protein Families | Transmembrane |
| Gene Summary | The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Amyotrophic lateral sclerosis-related VAPB P56S mutation differentially affects the function and survival of corticospinal and spinal motor neurons
,Leonardo Aliaga, Chen Lai, Jia Yu, Nikolai Chub, Hoon Shim, Lixin Sun, Chengsong Xie, Wan-Jou Yang, Xian Lin, Michael J. O'Donovan, and Huaibin Cai,
Hum. Mol. Genet., Jun 2013; 10.1093/hmg/ddt279.
,PubMed ID 23771029
[VAPB]
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VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis
,Kurt J. De Vos, Gábor M. Mórotz, Radu Stoica, Elizabeth L. Tudor, Kwok-Fai Lau, Steven Ackerley, Alice Warley, Christopher E. Shaw, and Christopher C.J. Miller,
Hum. Mol. Genet., Mar 2012; 21: 1299 - 1311.
[VAPB]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200517 | VAPB (Myc-DDK-tagged)-Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1 |
CNY 3600.00 |
|
| RC200517L1 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC200517L2 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, mGFP tagged |
CNY 6000.00 |
|
| RC200517L3 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC200517L4 | Lenti ORF clone of Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1, mGFP tagged |
CNY 6000.00 |
|
| RG200517 | VAPB (tGFP-tagged) - Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1 |
CNY 5200.00 |
