eRF1 (ETF1) (NM_004730) Human Untagged Clone
CAT＃: SC117181
ETF1 (untagged)-Human eukaryotic translation termination factor 1 (ETF1)
CNY 3656.00
CNY 7030.00
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | D5S1995; ERF; ERF1; RF1; SUP45L1; TB3-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117181 sequence for NM_004730 edited (data generated by NextGen Sequencing)
ATGGCGGACGACCCCAGTGCTGCCGACAGGAACGTGGAGATCTGGAAGATCAAGAAGCTC ATTAAGAGCTTGGAGGCGGCCCGCGGCAATGGCACCAGCATGATATCATTGATCATTCCT CCCAAAGACCAGATTTCACGAGTGGCAAAAATGTTAGCGGATGAGTTTGGAACTGCATCT AACATTAAGTCACGAGTAAACCGCCTTTCAGTCCTGGGAGCCATTACATCTGTACAACAA AGACTCAAACTTTATAACAAAGTACCTCCAAATGGTCTGGTTGTATACTGTGGAACAATT GTAACAGAAGAAGGAAAGGAAAAGAAAGTCAACATTGACTTTGAACCTTTCAAACCAATT AATACGTCATTGTATTTGTGTGACAACAAATTCCATACAGAGGCTCTTACAGCACTACTT TCAGATGATAGCAAGTTTGGATTCATTGTAATAGATGGTAGTGGTGCACTTTTTGGCACA CTCCAAGGAAACACAAGAGAAGTCCTGCACAAATTCACTGTGGATCTCCCAAAGAAACAC GGTAGAGGAGGTCAGTCAGCCTTGCGTTTTGCCCGTTTAAGAATGGAAAAGCGACATAAC TATGTTCGGAAAGTAGCAGAGACTGCTGTGCAGCTGTTTATTTCTGGGGACAAAGTGAAT GTGGCTGGTCTAGTTTTAGCTGGATCCGCTGACTTTAAAACTGAACTAAGTCAATCTGAT ATGTTTGATCAGAGGTTACAATCAAAAGTTTTAAAATTAGTTGATATATCCTATGGTGGT GAAAATGGATTCAACCAAGCTATTGAGTTATCTACTGAAGTCCTCTCCAACGTGAAATTC ATTCAAGAGAAGAAATTAATAGGACGATACTTTGATGAAATCAGCCAGGACACGGGCAAG TACTGTTTTGGCGTTGAAGATACACTAAAGGCTTTGGAAATGGGAGCTGTAGAAATTCTA ATAGTCTATGAAAATCTGGATATAATGAGATATGTTCTTCATTGCCAAGGCACAGAAGAG GAGAAAATTCTCTATCTAACTCCAGAGCAAGAAAAGGATAAATCTCATTTCACAGACAAA GAGACCGGACAGGAACATGAGCTTATCGAGAGCATGCCCCTGTTGGAATGGTTTGCTAAC AACTATAAAAAATTTGGAGCTACGTTGGAAATTGTCACAGATAAATCACAAGAAGGGTCT CAGTTTGTGAAAGGATTTGGTGGAATTGGAGGTATCTTGCGGTACCGAGTAGATTTCCAG GGAATGGAATACCAAGGAGGAGACGATGAATTTTTTGACCTTGATGACTACTAG Clone variation with respect to NM_004730.2 >OriGene 5' read for NM_004730 unedited
GTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGAGGGAGC CGGTGTCCTACGTCAGAGCCGCCGCCGCCGCGGAGCCGCCGCCGGGGAGGAGCAGCCGCT GCCGCCCAGGACTGGGCCCTTAGGGAGGAGGAGGCGAGAAGATGGCGGACGACCCCAGTG CTGCCGACAGGAACGTGGAGATCTGGAAGATCAAGAAGCTCATTAAGAGCTTGGAGGCGG CCCGCGGCAATGGCACCAGCATGATATCATTGATCATTCCTCCCAAAGACCAGATTTCAC GAGTGGCAAAAATGTTAGCGGATGAGTTTGGAACTGCATCTAACATTAAGTCACGAGTAA ACCGCCTTTCAGTCCTGGGAGCCATTACATCTGTACAACAAAGACTCAAACTTTATAACA AAGTACCTCCAAATGGTCTGGTTGTATACTGTGGAACAATTGTAACAGAAGAAGGAAAGG AAAAGAAAGTCAACATTGACTTTGAACCTTTCAAACCAATTAATACGTCATTGTATTTGT GTGACAACAAATTCCATACAGAGGCTCTTACAGCACTACTTTCAGATGATAGCAAGTTTG GATTCATTGTAATAGATGGTAGTGGTGCACTTTTTGGCACACTCCAAGGAAACACAAGAG AAGTCCTGCACAAATTCACTGTGGATCTCCCAAAGAAACACGGTAGAGGAGGTCAGTCAG CCTTGCGTTTTGCCCGTTTTAAGATGGAAAAGCGACATAACTATGTTCGGAAAGTAGCAG AGACTGCTGTGCAGCTNGTTATTTCTGGNGACAAAGTGATGTGGCTGGTCTAGTTTAGCT GGATCCGCTGACTTTAAAACTGACTAAGTCATCTGGATATGTTGATCAAAGGNTACATCA AAAGTTTAAAAATAGNTGTA >OriGene 3' read for NM_004730 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTCTTAAATATGGTTTAAT ACTCTTCTCCATTTCTGTACATCACAACACCAAGATTTTGCTGCTTAGTATCTCTCTGCA GAGGCTATAGAGAATGCAAAGGCTACGGTTTTCACCCCCTCTTATATGTGTTTGTATGTG TAAGTGTAATACATATCAATATATATTGATACACACATCAATATATAATGCAATATATAT CACCGAAGAGAACACATTGATTAAAGAAATACAAAAATTTGGCATCATTTCCAAACTTAA ATAGTAAAAATAAAAACTACAAAAGGAGCTGCATACCCTAAATGTATCATGTGAAACAAC AAGCATATTCAAAAATGTAAATTTACATCCAATTTCTCTGGTCTTGTCATATCACATCAG ACCCATTTACAATGGCAAAACCCTAAGGTACTGCTGTGAAGAGAGCATGTTTGCTCGCTC TTGGGTGTTCTGCAAACAAATAGCAGAGCCCAAAGCANAAAAGCCTGGTCCGGTGAAGTC TCCACGTTGGTGAACACCAAATAATGGACTCTTCTCACAGGGGGTTGAGACATCANGTTA CACATTGACAGACAGTATTTGGACCTCAGAGTACAGTGTGAGAACCAGAAACTTTACATT TAAGACATACTCCCTTCATTCNAGTGAAAACAGATATTGGAACGATANGCANGTCTGTAA CCCTGGAACAAGGAGCTGTGTCAGACCCATAAAGCTACGAGTGACTGAATCAAGTCAGTA AAGAACAGCAGTCAGGCACTAAAGTTGTAAAAGTACCCANTTGAAAACAAATGCACCCAC TACCCTATCAGTGNGNAGGTGATACCAATGACTTTNAAACNNCTTGNNCTTCAAGAGCAT TNGGNATACTTTTTNCATCGCTACACTGGGCACGTACTTTACACTTTGTTCACTGTACCA CCCTGGGCCCGCATGACCTGCATAAANCNTGGTTCAGNCTGACTTACGCCAGCTGTGAGC ATAGCTCAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004730 |
| Insert Size | 3780 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004730.1, NP_004721.1 |
| RefSeq Size | 3653 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 2107 |
| UniProt ID | P62495 |
| Domains | eRF1_1, eRF1_2, eRF1_3 |
| Gene Summary | This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220387 | ETF1 (Myc-DDK-tagged)-Human eukaryotic translation termination factor 1 (ETF1) |
CNY 3656.00 |
|
| RC220387L1 | Lenti ORF clone of Human eukaryotic translation termination factor 1 (ETF1), Myc-DDK-tagged |
CNY 6056.00 |
|
| RC220387L2 | Lenti ORF clone of Human eukaryotic translation termination factor 1 (ETF1), mGFP tagged |
CNY 5890.00 |
|
| RC220387L3 | Lenti ORF clone of Human eukaryotic translation termination factor 1 (ETF1), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC220387L4 | Lenti ORF clone of Human eukaryotic translation termination factor 1 (ETF1), mGFP tagged |
CNY 5890.00 |
|
| RG220387 | ETF1 (tGFP-tagged) - Human eukaryotic translation termination factor 1 (ETF1) |
CNY 5256.00 |
