EOMES (NM_005442) Human Untagged Clone
CAT＃: SC116738
EOMES (untagged)-Human eomesodermin (EOMES)
CNY 7544.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TBR2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005442 edited
ATGCAGTTAGGGGAGCAGCTCTTGGTGAGCTCAGTGAACCTGCCTGGCGCGCACTTCTAC CCGCTGGAGAGTGCGCGAGGCGGCAGCGGCGGGAGCGCTGGCCACCTCCCCAGCGCGGCC CCCTCTCCTCAGAAGTTGGACTTAGACAAAGCGTCCAAGAAGTTTTCCGGCAGTCTCTCC TGCGAGGCGGTGAGCGGGGAGCCCGCAGCCGCCAGCGCAGGGGCCCCCGCGGCCATGCTT AGTGACACCGACGCCGGGGACGCATTTGCCAGCGCTGCGGCAGTGGCCAAGCCGGGGCCC CCGGACGGCCGCAAGGGCTCCCCCTGCGGGGAGGAGGAGCTGCCCTCCGCCGCTGCAGCC GCCGCCGCCGCCGCCGCCGCGGCTGCGGCCACTGCGCGCTACTCCATGGACAGCCTGAGC TCCGAGCGGTACTACCTCCAGTCCCCCGGTCCTCAGGGGTCGGAGCTGGCTGCGCCCTGC TCACTCTTCCCGTACCAGGCGGCGGCTGGGGCGCCCCACGGACCTGTGTACCCGGCTCCT AACGGGGCGCGCTACCCCTACGGCTCCATGCTGCCCCCCGGCGGCTTCCCCGCGGCTGTG TGCCCACCCGGGAGGGCGCAGTTCGGCCCAGGAGCCGGTGCGGGCAGTGGCGCGGGCGGT AGCAGCGGCGGGGGCGGCGGCCCGGGCACCTATCAGTACAGCCAGGGGGCTCCGCTCTAC GGGCCGTACCCTGGAGCCGCAGCGGCGGGATCTTGCGGAGGACTGGGGGGCCTGGGGGTT CCAGGTTCTGGCTTCCGTGCCCACGTCTACCTGTGCAACCGGCCTCTGTGGCTCAAATTC CACCGCCACCAAACTGAGATGATCATTACGAAACAGGGCAGGCGCATGTTTCCTTTCTTG AGCTTCAACATAAACGGACTCAATCCCACTGCCCACTACAATGTGTTCGTAGAGGTGGTG CTGGCGGACCCCAACCACTGGCGCTTCCAGGGGGGCAAATGGGTGACCTGTGGCAAAGCC GACAATAACATGCAGGGCAACAAAATGTATGTTCACCCAGAGTCTCCTAATACTGGTTCC CACTGGATGAGACAGGAGATTTCATTCGGGAAATTAAAACTCACCAATAACAAAGGCGCA AATAACAACAACACCCAGATGATAGTCTTACAATCCTTACACAAATACCAACCCCGACTG CATATTGTTGAAGTTACAGAGGATGGCGTGGAGGACTTGAATGAGCCCTCAAAGACCCAG ACTTTTACCTTCTCAGAAACGCAATTCATTGCAGTGACTGCCTACCAAAACACCGATATT ACTCAACTAAAGATTGATCATAACCCCTTTGCAAAAGGCTTCAGAGACAACTATGATTCA TCCCATCAGATTGTCCCTGGAGGTCGGTACGGCGTTCAATCCTTCTTCCCGGAGCCCTTT GTCAACACTTTACCTCAAGCCCGCTATTATAATGGCGAGAGAACCGTGCCACAGACCAAC GGCCTCCTTTCACCCCAACAGAGCGAAGAGGTGGCCAACCCTCCCCAGCGGTGGCTTGTC ACGCCTGTCCAGCAACCTGGGACCAACAAACTAGACATCAGTTCCTATGAATCTGAATAT ACTTCTAGCACATTGCTCCCATATGGCATTAAATCCTTGCCCCTTCAGACATCCCATGCC CTGGGGTATTACCCAGACCCAACCTTTCCTGCAATGGCAGGGTGGGGAGGTCGAGGTTCT TACCAGAGGAAGATGGCAGCTGGACTACCATGGACCTCCAGAACAAGCCCCACTGTGTTC TCTGAAGATCAGCTCTCCAAGGAGAAAGTGAAAGAGGAAATTGGCTCTTCTTGGATAGAG ACACCCCCTTCCATCAAATCTCTAGATTCCAATGATTCAGGAGTATACACCAGTGCTTGT AAGCGAAGGCGGCTGTCTCCTAGCAACTCCAGTAATGAAAATTCACCCTCCATAAAGTGT GAGGACATTAATGCTGAAGAGTATAGTAAAGACACCTCAAAAGGCATGGGAGGGTATTAT GCTTTTTACACAACTCCCTAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005442 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005442.2, NP_005433.2 |
| RefSeq Size | 2756 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 8320 |
| UniProt ID | O95936 |
| Domains | T-box |
| Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221014 | EOMES (Myc-DDK-tagged)-Human eomesodermin (EOMES) |
CNY 7536.00 |
|
| RC221014L1 | Lenti ORF clone of Human eomesodermin (EOMES), Myc-DDK-tagged |
CNY 9936.00 |
|
| RC221014L2 | Lenti ORF clone of Human eomesodermin (EOMES), mGFP tagged |
CNY 9936.00 |
|
| RC221014L3 | Lenti ORF clone of Human eomesodermin (EOMES), Myc-DDK-tagged |
CNY 9936.00 |
|
| RC221014L4 | Lenti ORF clone of Human eomesodermin (EOMES), mGFP tagged |
CNY 9120.00 |
|
| RG221014 | EOMES (tGFP-tagged) - Human eomesodermin (EOMES) |
CNY 9136.00 |
