GLCNE (GNE) (NM_005476) Human Untagged Clone
CAT＃: SC116716
GNE (untagged)-Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2
CNY 5296.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DMRV; GLCNE; IBM2; NM; Uae1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116716 sequence for NM_005476 edited (data generated by NextGen Sequencing)
ATGGAGAAGAATGGAAATAACCGAAAGCTGCGGGTTTGTGTTGCTACTTGTAACCGTGCA GATTATTCTAAACTTGCCCCGATCATGTTTGGCATTAAAACCGAACCTGAGTTCTTTGAA CTTGATGTTGTGGTACTTGGCTCTCACCTGATAGATGACTATGGAAATACATATCGAATG ATTGAACAAGATGACTTTGACATTAACACCAGGCTACACACAATTGTGAGGGGAGAAGAT GAGGCAGCCATGGTGGAGTCAGTAGGCCTGGCCCTAGTGAAGCTGCCAGATGTCCTTAAT CGCCTGAAGCCTGATATCATGATTGTTCATGGAGACAGGTTTGATGCCCTGGCTCTGGCC ACATCTGCTGCCTTGATGAACATCCGAATCCTTCACATTGAAGGTGGGGAAGTCAGTGGG ACCATTGATGACTCTATCAGACATGCCATAACAAAACTGGCTCATTATCATGTGTGCTGC ACCCGCAGTGCAGAGCAGCACCTGATATCCATGTGTGAGGACCATGATCGCATCCTTTTG GCAGGCTGCCCTTCCTATGACAAACTTCTCTCAGCCAAGAACAAAGACTACATGAGCATC ATTCGCATGTGGCTAGGTGATGATGTAAAATCTAAAGATTACATTGTTGCACTACAGCAC CCTGTGACCACTGACATTAAGCATTCCATAAAAATGTTTGAATTAACATTGGATGCACTT ATCTCATTTAACAAGCGGACCCTAGTCCTGTTTCCAAATATTGACGCAGGGAGCAAAGAG ATGGTTCGAGTGATGCGGAAGAAGGGCATTGAGCATCATCCCAACTTTCGTGCAGTTAAA CACGTCCCATTTGACCAGTTTATACAGTTGGTTGCCCATGCTGGCTGTATGATTGGGAAC AGCAGCTGTGGGGTTCGAGAAGTTGGAGCTTTTGGAACACCTGTGATCAACCTGGGAACA CGTCAGATTGGAAGAGAAACAGGGGAGAATGTTCTTCATGTCCGGGATGCTGACACCCAA GACAAAATATTGCAAGCACTGCACCTTCAGTTTGGTAAACAGTACCCTTGTTCAAAGATA TATGGGGATGGAAATGCTGTTCCAAGGATTTTGAAGTTTCTCAAATCTATCGATCTTCAA GAGCCACTGCAAAAGAAATTCTGCTTTCCTCCTGTGAAGGAGAATATCTCTCAAGATATT GACCATATTCTTGAAACTCTAAGTGCCTTGGCCGTTGATCTTGGCGGGACGAACCTCCGA GTTGCAATAGTCAGCATGAAGGGTGAAATAGTTAAGAAGTATACTCAGTTCAATCCTAAA ACCTATGAAGAGAGGATTAATTTAATCCTACAGATGTGTGTGGAAGCTGCAGCAGAAGCT GTAAAACTGAACTGCAGAATTTTGGGAGTAGGCATTTCCACAGGTGGCCGTGTAAATCCT CGGGAAGGAATTGTGCTGCATTCAACCAAACTGATCCAAGAGTGGAACTCTGTGGACCTT AGGACCCCCCTTTCTGACACTTTGCATCTCCCTGTGTGGGTAGACAATGATGGCAACTGT GCTGCCCTGGCGGAAAGGAAATTTGGCCAAGGAAAGGGACTGGAAAACTTTGTTACACTT ATCACAGGCACAGGAATCGGTGGTGGAATTATCCATCAGCATGAATTGATCCACGGAAGC TCCTTCTGTGCTGCAGAACTGGGCCACCTTGTTGTGTCTCTGGATGGGCCTGATTGTTCC TGTGGAAGCCATGGGTGCATTGAAGCATACGCCTCTGGAATGGCCTTGCAGAGGGAGGCA AAAAAGCTCCATGATGAGGACCTGCTCTTGGTGGAAGGGATGTCAGTGCCAAAAGATGAG GCTGTGGGTGCGCTCCATCTCATCCAAGCTGCGAAACTTGGCAATGCGAAGGCCCAGAGC ATCCTAAGAACAGCTGGAACAGCTTTGGGTCTTGGGGTTGTGAACATCCTCCATACCATG AATCCCTCCCTTGTGATCCTCTCCGGAGTCCTGGCCAGTCACTATATCCACATTGTCAAA GACGTCATTCGCCAGCAGGCCTTGTCCTCCGTGCAGGACGTGGATGTGGTGGTTTCGGAT TTGGTTGACCCCGCCCTGCTGGGTGCTGCCAGCATGGTTCTGGACTACACAACACGCAGG ATCTACTAG Clone variation with respect to NM_005476.5 >OriGene 5' read for NM_005476 unedited
CCCATTCCCCTCGAGGCTAAACCATTAAACGCTGCTCTGCTCATTATTTCATACTCTACT AGGGTACAGAGCTCGTGCTTCGGGATGGAAACCTATGGTTATCTGCAGAGGGAGTCATGC TTTCAAGGACCTCATGAACTCTATTTTAAGAACCTCTCAAAACGAAACAAGCTAAACTGN GNTTAAGAATGGAAATAACCTTTAGCTGCGGGTTTGTGTTGCTACTTGTAACCGTGCAGA TTATTCTAAACTTGCCCCTATTATGTTTGGCATTAAAACCGAACCTGAGTTCTTTGAACT TGATGTTGTGGTACTTGGCTCTCACCTGATAGATGACTATGGAAATACATATCGAATGAT TGAACAAGATGACTTTGACATTAACACCAGGCTACACACAATTGTGAGGGGAGAAGATGA GGCAGCCATGGTGGAGTCAGTAGGCCTGGCCCTAGTGAAGCTGCCAGATGTCCTTAATCG CCTGAAGCCTGATATCATGATTGTTCATGGAGACAGGTTTGATGCCCTGGCTCTGGCCAC ATCTGCTGCCTTGATGAACATCCGAATCCTTCACATTGAAGGTGGGGAAGTCAGTGGGAC CATTGATGACTCTATCAGACATGCCATAACAAAACTGGCTCATTATCATGTGTGCTGCAC CCGCAGTGCATAGCAGCACCTGATATCCATGTGTGAGGACCATGATCGCATCCTTTTGGC AAGCTGCCCTTCCTATGACAAACTTCTCTCAGCCAAGACAAAGACTACCTGAGCATCATT CGCATGTGGCTAAGTGATGATGTAAAATCTAAAGATACATTGTTGCACTACGCACCCTGT GACCACTGACTT >OriGene 3' read for NM_005476 unedited
NAATCCTGNACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTAGTAGAGACG GGGTTTCACCATGTTTCACCATGTTAGCCAGGGCGGTCTCGATCTCCTGACCTCGTGATC CGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCCGGCCTG GAAGGATTATTAATGCAAACTTCAGGATGAAGTGATATCCCAGGCAAGGCCTGAAGGTCT CAGTGGTATTAATACATTAGTAAGCAGAGTTCTAAGAAGGCTTCCTCTCTATTATTGTAG CTGCTTTGGCACAGAAAATTGTGACTGTAACTTACAGGGTTTGAGCTAAAATGACCCCTA GTAAGAAGACATCAGAAAGAATAGCATCTACAAAAATAGGAGTGGGGAAAGGTGACTCTG GAAGAGGAGACCAAAAGTGAAAACATTTCTCTGCCAAAGTCACCTGCAGTTCAATACCAG ATTTGATTGTTAAGAAACGGTCATCCTAAAGACAAGAACTTGATTCCACTCAGGAGCTCT GGAGAGAAGGTCCATGTCTGTTCCTGGAGGTCTAGTAGATCCTGCGTGTTGTGTAGTCCA GAACCATGCTGGCAGCACCCANCAGGGCCGGGTCAACCAAATCCGAACCACCACATCCAC GTCCTGCACGGAGGACAAGGCCTGCTGGCGAATGACGTCTTTGACAATGGGGATATAGTG ACTGGCCCAGACTCCGGAGAGATCACCAGGGAGGAATTCTGGTTTGGGGGTGTTCACACC CCCAAAACCCAAGCTGTTCCGACCTTTCTTAGGAGCCCCGGGCCTTCCCCTTGCCAGTTT CCCAGCTCGGAGAGAAGGAGCCCCTCCCCGTGCTACATTTTTTTGCCGCAACGCCCTCCA CCCACACCGAGTACA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005476 |
| Insert Size | 2940 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005476.3, NP_005467.1 |
| RefSeq Size | 3760 bp |
| RefSeq ORF | 2169 bp |
| Locus ID | 10020 |
| UniProt ID | Q9Y223 |
| Domains | ROK, Epimerase_2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains a different 5' terminal exon compared to transcript variant 1, which results in translation initiation from an in-frame downstream AUG, and a protein (isoform 2) with a shorter N-terminus compared to isoform 1. This variant has been called GNE1 in the scientific literature. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222626 | GNE (Myc-DDK-tagged)-Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2 |
CNY 5288.00 |
|
| RC222626L1 | Lenti ORF clone of Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, Myc-DDK-tagged |
CNY 7688.00 |
|
| RC222626L2 | Lenti ORF clone of Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mGFP tagged |
CNY 7700.00 |
|
| RC222626L3 | Lenti ORF clone of Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, Myc-DDK-tagged |
CNY 7688.00 |
|
| RC222626L4 | Lenti ORF clone of Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2, mGFP tagged |
CNY 7700.00 |
|
| RG222626 | GNE (tGFP-tagged) - Human glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 2 |
CNY 6888.00 |
