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AIM (CD5L) (NM_005894) Human Untagged Clone
CAT＃: SC116428
CD5L (untagged)-Human CD5 molecule-like (CD5L)
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AIM; API6; CT-2; hAIM; PRO229; SP-ALPHA; Spalpha |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC116428 sequence for NM_005894 edited (data generated by NextGen Sequencing)
ATGGCTCTGCTATTCTCCTTGATCCTTGCCATTTGCACCAGACCTGGATTCCTAGCGTCT CCATCTGGAGTGCGGCTGGTGGGGGGCCTCCACCGCTGTGAAGGGCGGGTGGAGGTGGAA CAGAAAGGCCAGTGGGGCACCGTGTGTGATGACGGCTGGGACATTAAGGACGTGGCTGTG TTGTGCCGGGAGCTGGGCTGTGGAGCTGCCAGCGGAACCCCTAGTGGTATTTTGTATGAG CCACCAGCAGAAAAAGAGCAAAAGGTCCTCATCCAATCAGTCAGTTGCACAGGAACAGAA GATACATTGGCTCAGTGTGAGCAAGAAGAAGTTTATGATTGTTCACATGATGAAGATGCT GGGGCATCGTGTGAGAACCCAGAGAGCTCTTTCTCCCCAGTCCCAGAGGGTGTCAGGCTG GCTGACGGCCCTGGGCATTGCAAGGGACGCGTGGAAGTGAAGCACCAGAACCAGTGGTAT ACCGTGTGCCAGACAGGCTGGAGCCTCCGGGCCGCAAAGGTGGTGTGCCGGCAGCTGGGA TGTGGGAGGGCTGTACTGACTCAAAAACGCTGCAACAAGCATGCCTATGGCCGAAAACCC ATCTGGCTGAGCCAGATGTCATGCTCAGGACGAGAAGCAACCCTTCAGGATTGCCCTTCT GGGCCTTGGGGGAAGAACACCTGCAACCATGATGAAGACACGTGGGTCGAATGTGAAGAT CCCTTTGACTTGAGACTAGTAGGAGGAGACAACCTCTGCTCTGGGCGACTGGAGGTGCTG CACAAGGGCGTATGGGGCTCTGTCTGTGATGACAACTGGGGAGAAAAGGAGGACCAGGTG GTATGCAAGCAACTGGGCTGTGGGAAGTCCCTCTCTCCCTCCTTCAGAGACCGGAAATGC TATGGCCCTGGGGTTGGCCGCATCTGGCTGGATAATGTTCGTTGCTCAGGGGAGGAGCAG TCCCTGGAGCAGTGCCAGCACAGATTTTGGGGGTTTCACGACTGCACCCACCAGGAAGAT GTGGCTGTCATCTGCTCAGGATAG Clone variation with respect to NM_005894.2 >OriGene 5' read for NM_005894 unedited
CCCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG AGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAT TCGGCACGAGACTTGCAGCTAAAACTAAATATTGCTGCTTGGGGACCTCCTTCTAGCCTT AAATTTCAGCTCATCACCTTCACCTGCCTTGGTCATGGCTCTGCTATTCTCCTTGATCCT TGCCATTTGCACCAGACCTGGATTCCTAGCGTCTCCATCTGGAGTGCGGCTGGTGGGGGG CCTCCACCGCTGTGAAGGGCGGGTGGAGGTGGAACAGAAAGGCCAGTGGGGCACCGTGTG TGATGACGGCTGGGACATTAAGGACGTGGCTGTGTTGTGCCGGGAGCTGGGCTGTGGAGC TGCCAGCGGAACCCCTAGTGGTATTTTGTATGAGCCACCAGCAGAAAAAGAGCAAAAGGT CCTCATCCAATCAGTCAGTTGCACAGGAACAGAAGATACATTGGCTCAGTGTGAGCAAGA AGAAGTTTATGATTGTTCACATGATGAAGATGCTGGGGCATCGTGTGAGAACCCAGAGAG CTCTTTCTCCCCAGTCCCAGAGGGTGTCAGGCTGGCTGACGGCCCTGGGCATTGCAAGGG ACGCGTGGAAGTGAAGCACCAGAACCAGTGGTATACCGTGTGCCAGACAGGCTGGAGCCT CCGGGCCGCANAGGTGGTGTGCCCGCAGCTGGNNATGTGGAGGGCTGTACTGACTCAAAA ACGCTGNCACAAGCATGCCTATGGNCCGAAACCCATCTGGCTGAGCCAGATGTCATGCTC AGGACGAGAAGCAACCCTTCANGGATGCCCTNCTGGGCCCTGGGGAAGNAACACTGCACC ATGATGAAGACACGTGGGTCGATGTGAAGATCCTTTGACTGNGACTAGTNAGAGGAGACA ACTCTGCTCTGGCGACC >OriGene 3' read for NM_005894 unedited
GAACATGCTTACATAACCCANNNGCTGAAGGGTAAATGTGTTAAAGCTAGCCGTATAATA AGTACTCATCCTATCCTCAGAATCTAAAGTTGTCAATTTTTACAATCAAGTCTTGATTCT CTTATTTCTGTCTTGCCTCAAGCTTACAGGCCCCAAAGTCTCAGTTGATAAAGTGAAAAT GATATTTTTCACTTTCGCTTGGCATAAGACACAACTTTAGCCCTCCCTGATCTCTTTCTG CCTCTTTCTTGACCTTTTCCCAGTAACCAATATATTTTTCTTTGAATAAAGTAAAATGTA TTTTTGACATTCCTCTCATTAAATGTCAACCATGTTGGCAGGTTGTATAGGGATGGACAA CAAAGATTTTTATTGGGGCAATCAGAGGATGAAACATCATTTTTTACACGTCATGAAAAG GTCTAGGATTATAGGACGCTGCTCTGCTCCTGAAAGAATTGTCTACCTACACTAAGAAAT AACAGAATAGGGAGGTGTTGCTATTGTGGTCACCTGAAGCTTGAGGAAAAAAAAAAAAAG TAGTCCAATCAAAAGAATTCTAAACTTTCCCCCAAGTTGCAAGATATTGTCTACCCACCT TTTCTTTCAAATGTTCCTTTCATTGTTTCATTCCTTTAATGCTTGCAGACCTAGATTAGT AATACGACCTATATCAGTAATGTTTGCCGACCTAGACCTTAGTAATGGGCTGCACATCTG ACCCAAGTGACAAGCTTGAGGATTCCAGGCGCCTTGTGGAAAGGCCGGGAAGGCCCGTAC CAGTGTAAAGGGCAAGGGTGGGCACCGGATACCTGGAAGCTTATCTCCCCCGCAAGAGGG AACCCACCACTCACTTTACAGGCAGCAAAGGGGGCTGCTGTCCCTTGGAGACAAGCATAA CCAAGGTTTAAACTCCGGAGGGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005894 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005894.1, NP_005885.1 |
| RefSeq Size | 2181 bp |
| RefSeq ORF | 1044 bp |
| Locus ID | 922 |
| UniProt ID | O43866 |
| Domains | SR |
| Protein Families | Druggable Genome, Secreted Protein |
| Gene Summary | Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (By similarity). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:24295828). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (PubMed:16030018, PubMed:24223991, PubMed:24583716, PubMed:25713983).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1, which differs only in the last aa compared to isoform 2. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206528 | CD5L (Myc-DDK-tagged)-Human CD5 molecule-like (CD5L) |
CNY 3,656.00 |
|
| RC206528L1 | Lenti ORF clone of Human CD5 molecule-like (CD5L), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206528L2 | Lenti ORF clone of Human CD5 molecule-like (CD5L), mGFP tagged |
CNY 5,890.00 |
|
| RC206528L3 | Lenti ORF clone of Human CD5 molecule-like (CD5L), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206528L4 | Lenti ORF clone of Human CD5 molecule-like (CD5L), mGFP tagged |
CNY 5,890.00 |
|
| RG206528 | CD5L (tGFP-tagged) - Human CD5 molecule-like (CD5L) |
CNY 4,370.00 |
