SPATA2 (NM_006038) Human Untagged Clone
CAT＃: SC116373
SPATA2 (untagged)-Human spermatogenesis associated 2 (SPATA2), transcript variant 1
CNY 3880.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PD1; PPP1R145; tamo |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006038, the custom clone sequence may differ by one or more nucleotides
ATGGGGAAGCCCAGTTCAATGGATACTAAATTCAAGGATGACTTATTTCGGAAGTACGTGCAGTTCCATG AGAGCAAAGTGGATACCACCACCAGCAGGCAGCGGCCTGGCAGCGATGAGTGCCTGCGGGTGGCAGCCTC AACCCTGCTCAGCCTGCACAAGGTGGATCCCTTTTATCGATTCCGGCTGATCCAGTTCTATGAGGTGGTG GAGAGCTCCTTGCGCTCGCTCAGCTCCTCTAGCCTGCGGGCTCTGCACGGCGCCTTCAGCATGCTGGAGA CGGTGGGCATCAACCTCTTCCTCTACCCGTGGAAGAAGGAATTCAGAAGCATCAAGACCTACACGGGCCC TTTTGTTTATTATGTCAAGTCGACATTACTGGAAGAGGACATCCGAGCCATCCTGAGCTGCATGGGCTAC ACACCTGAGCTGGGCACTGCATACAAGCTCAGAGAGCTCGTGGAGACCCTCCAGGTGAAGATGGTCTCCT TTGAGCTCTTTCTGGCCAAAGTCGAGTGTGAGCAGATGCTAGAAATCCACTCACAAGTGAAGGACAAGGG CTACTCCGAGCTGGACATTGTGAGCGAGCGCAAGAGCAGTGCAGAGGATGTGCGCGGCTGCTCGGACGCC CTGCGGCGGCGGGCAGAGGGCCGGGAGCACCTGACGGCCTCCATGTCACGAGTGGCACTCCAGAAGTCGG CCAGCGAGCGGGCGGCCAAGGACTACTACAAGCCCCGCGTGACCAAGCCCTCGAGGTCAGTGGATGCCTA TGACAGCTACTGGGAGAGCCGGAAGCCACCCCTGAAGGCCTCATTGAGTCTTCGGAAGGAGCCTGTGGCA ACGGATGTGGGGGACGACCTCAAGGATGAGATCATCCGCCCATCCCCTTCGCTGCTGACCATGGCCAGCT CCCCCCACGGCAGCCCGGATGTGCTTCCACCCGCCTCCCCCAGCAACGGCCCGGCCCTGCTGCGCGGTAC CTACTTCTCCACTCAGGATGACGTGGATCTGTACACAGACTCTGAACCCAGGGCCACCTACCGTCGGCAG GATGCTCTGCGGCCGGATGTGTGGCTGCTCAGAAACGATGCCCACTCCCTCTACCACAAGCGCTCGCCCC CTGCCAAAGAGTCCGCCCTCTCCAAGTGCCAAAGCTGCGGGCTGTCCTGCAGCTCCTCCCTCTGCCAGCG CTGTGACAGCCTGCTCACCTGTCCTCCAGCTTCCAAGCCCAGCGCCTTCCCCAGCAAGGCCTCGACTCAT GACAGCCTGGCCCACGGGGCATCTCTGCGGGAGAAGTACCCAGGCCAGACTCAGGGCCTCGACCGCCTCC CGCACCTTCACTCCAAATCCAAGCCCTCCACCACGCCCACTTCCCGCTGTGGCTTCTGCAACCGCCCAGG CGCCACCAACACCTGCACCCAGTGTTCAAAAGTCTCATGTGACGCCTGCCTCAGCGCTTACCATTATGAC CCCTGCTACAAAAAGAGTGAGCTGCACAAGTTCATGCCCAACAACCAGCTGAACTACAAGTCCACCCAGC TCTCCCATCTCGTGTACAGATAG >OriGene 5' read for NM_006038 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGCTGGCCCCCGG GTACTGGTCTCAGTACCCCTGCAGCCGGCCGGAGAGGCTAGAGCCCGCGGGGCCCGGCTC CGGCGGCAGCCGCGCCTCTCGCTTGCTTCCCTCGGCCGGGCCGTCCTCGGCGGCAGTGTC AGGAGCACTCCGCTGGTCCAGGCGGCAACATGTCCATGCTTTTAGTGATGTCCCTGCCTC TAATCCAGAAGCCATGGAGGAGATAAGGTAGCCCCCCTCGATCGGATGGGGAAGCCCAGT TCAATGGATACTAAATTCAAGGATGACTTATTTCGGAAGTACGTGCAGTTCCATGAGAGC AAAGTGGATACCACCACCAGCAGGCAGCGGCCTGGCAGCGATGAGTGCCTGCGGGTGGCA GCCTCAACCCTGCTCAGCCTGCACAAGGTGGATCCCTTTTATCGATTCCGGCTGATCCAG TTCTATGAGGTGGTGGAGAGCTCCTTGCGCTCGCTCAGCTCCTCTAGCCTGCGGGCTCTG CACGGCGCCTTCAGCATGCTGGAGACGGTGGGCATCAACCTCTTCCTCTACCCGTGGAAG AAGGAATTCAGAAGCATCAAGACCTACACGGGCCCTTTTGTTTATTATGTCAAGTCGACA TTACTGGAAGAGGACATCCGAGCCATCCTGAGCTGCATGGGCTACACACCTGAGCTGGGC ACTGCATACAAGCTCAGAGAGCTCGTGGAGACCCTCCAGGTGAANGATGGTCTCCTTTGA GCTCTTTCTGGCCAAAGTCGAGTGTGAGCAGATGCTAGAAATCCACTCACAGTGAAGGAC AAGGGCTACTCCGAGCTGGACATTGTGAGCGAGCGCAGAGCAGTGCANNAGGATGTGCGC GGGCTGCTCGACCCCTGCGGCGCGNNNCANAAGGGCCGGAGCACCTGACGNNCTCTGNTN ACGAGTGCCTTTCCANAAATCGGCACCGAGCGGC >OriGene 3' read for NM_006038 unedited
ATGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAACAATGNGAAGA CACTTTATTATTATTATTTTTAATCATCTCTCAACATTTCAACTCTTGGTAGAAATCAAA TGTAAAACATCTGCATTTTTAAATTAGCATGTATTTAACTATCTCAATCACAGCAACCTC ATCCAGTAAGATGCACACCTGGGAGCAGGGCTGTAGTATTTCGTATTCCAGTCTGTGACC ACAGTGCGTCCGTGATGACAGGTACACTGGACTTTCCCTCCTAGGATGTGTTAGTCATTC CTGCTTTTGTCCATAGGGTAAGTTACGTGGCATCACGGTTGGTTAGAAAGAGTTAGTTAC TGTAATGCTGGCGCAATCTGGAAATGGAAAACTAGAAAACTAGAGCTCTCAGGAGTGGGC CAGGCATTGCCGATTCTGAAATGTGAAAAGGAAGAACATCAAAGATGAATGCCCTGCTAG GCTGGTCTTAGTAATAAAGTTGGTTGAAATTCAGCTTAATATACACACTGTACATCTACA TGTGATCTACCAGACAGATATATATATATATATATATATATATATATATATATACATATA TATTAAAAAGAGAGCCCTAGAAGATTTGGAAAACCTTAAAATCACTCAACTGAGTTGGAA GAACATCACCAAATTCACAGTGGCCTCAGGATTCAGCCAGACTTAAACTTGCTCAAGAGC TGAATAACTTTTTCCCAGAAAAGCCCACTGCCTATAAACTCCTTAACATTGTGTACATCA CACACAGTAATGGCTAAAAATAGGTAGTCCACAGGAGCGGCTTCATTTTCAACTCTTGGC CCCTCTCGTTCTGTCCCCCACACGGAAATCCAGCGTTGCCATTCACAGCAAAATTGAGGG CCCCCTAGTCGGCAACCTTAGCCTACTTGACACTTGAGGGCCGCCCTTTGGGGGCCCAGC CGACCCCGCCACTTTTAGCAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006038 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006038.2, NP_006029.1 |
| RefSeq Size | 4058 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 9825 |
| UniProt ID | Q9UM82 |
| Gene Summary | Bridging factor that mediates the recruitment of CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis (PubMed:27307491, PubMed:27458237, PubMed:27545878, PubMed:27591049). Acts as a direct binding intermediate that bridges RNF31/HOIP, the catalytic subunit of the LUBAC complex, and the deubiquitinase (CYLD), thereby recruiting CYLD to the TNF-R1 signaling complex (TNF-RSC) (PubMed:27458237, PubMed:27545878, PubMed:27591049). Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked deubiquitinase activities of CYLD (PubMed:27458237, PubMed:27591049). Controls the kinase activity of RIPK1 and TNF-alpha-induced necroptosis by promoting 'Met-1'-linked deubiquitination of RIPK1 by CYLD (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) and variant 2 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203482 | SPATA2 (Myc-DDK-tagged)-Human spermatogenesis associated 2 (SPATA2), transcript variant 1 |
CNY 3872.00 |
|
| RC203482L3 | Lenti ORF clone of Human spermatogenesis associated 2 (SPATA2), transcript variant 1, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC203482L4 | Lenti ORF clone of Human spermatogenesis associated 2 (SPATA2), transcript variant 1, mGFP tagged |
CNY 6460.00 |
|
| RG203482 | SPATA2 (tGFP-tagged) - Human spermatogenesis associated 2 (SPATA2), transcript variant 1 |
CNY 5472.00 |
