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Epac1 (RAPGEF3) (NM_006105) Human Untagged Clone
CAT＃: SC116327
RAPGEF3 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006105, the custom clone sequence may differ by one or more nucleotides
ATGGTGTTGAGAAGGATGCACCGGCCCCGAAGCTGCTCCTACCAGCTGCTGCTGGAGCACCAGCGTCCGA GCTGCATCCAGGGGCTGCGCTGGACACCACTCACCAACAGCGAGGAGTCCCTGGATTTCAGCGAGAGCCT GGAGCAGGCCTCCACAGAGCGGGTGCTCAGGGCTGGGAGGCAGCTGCATCGGCATCTGCTGGCCACCTGC CCAAACCTCATCCGAGACCGGAAGTACCACCTTAGGCTCTATCGGCAGTGCTGCTCTGGCCGGGAGCTGG TGGATGGGATCTTGGCCCTGGGACTTGGGGTCCATTCCCGGAGCCAAGTTGTGGGAATCTGCCAGGTGCT GCTGGATGAAGGTGCCCTCTGCCATGTGAAACACGACTGGGCCTTCCAGGACCGAGATGCCCAATTCTAC CGGTTCCCCGGGCCCGAGCCCGAGCCCGTGGGAACTCATGAGATGGAGGAGGAGTTGGCCGAAGCTGTGG CCCTGCTCTCCCAGCGGGGGCCTGACGCCCTGCTCACTGTGGCACTTCGAAAGCCCCCAGGTCAGCGCAC GGATGAAGAGCTGGACCTCATCTTTGAGGAGCTGCTGCACATCAAGGCTGTGGCCCACCTCTCCAACTCG GTGAAGCGAGAATTAGCGGCTGTTCTGCTCTTTGAACCACACAGCAAGGCAGGGACCGTGTTGTTCAGCC AGGGGGACAAGGGCACTTCGTGGTACATTATCTGGAAGGGATCTGTCAACGTGGTGACCCATGGCAAGGG GCTGGTGACCACCCTGCATGAGGGAGATGATTTTGGACAGCTGGCTCTGGTGAATGATGCACCCCGGGCA GCCACCATCATCCTGCGAGAAGACAACTGTCATTTCCTGCGTGTGGACAAGCAGGACTTCAACCGTATCA TCAAGGATGTGGAGGCAAAGACCATGCGGCTGGAAGAACATGGCAAAGTGGTGCTGGTGCTGGAGAGAGC CTCTCAGGGCGCCGGCCCTTCCCGACCCCCAACCCCAGGCAGGAACCGGTATACAGTGATGTCTGGCACC CCAGAGAAGATCCTAGAGCTTCTGTTGGAGGCCATGGGACCAGATTCCAGTGCTCATGACCCAACAGAGA CATTCCTCAGCGACTTCCTCCTGACCCACAGGGTCTTCATGCCCAGCGCCCAACTCTGCGCTGCCCTTCT GCACCACTTCCATGTGGAGCCTGCGGGTGGCAGCGAGCAGGAGCGCAGCACCTACGTCTGCAACAAGAGG CAGCAGATCTTGCGGCTGGTCAGCCAGTGGGTGGCCCTGTATGGCTCCATGCTCCACACTGACCCTGTGG CCACCAGCTTCCTCCAGAAACTCTCAGACCTGGTGGGCAGGGACACCCGACTCAGCAACCTGCTGAGGGA GCAGTGGCCAGAGAGGCGGCGATGCCACAGGTTGGAGAATGGCTGTGGGAATGCATCTCCTCAGATGAAG GCCCGGAACTTGCCTGTTTGGCTCCCCAACCAGGACGAGCCCCTTCCTGGCAGCAGCTGTGCCATCCAAG TTGGGGATAAAGTCCCCTATGACATCTGCCGGCCAGACCACTCAGTGTTGACCCTGCAGCTGCCTGTGAC AGCCTCCGTGAGAGAGGTGATGGCAGCGTTGGCCCAGGAGGATGGCTGGACCAAGGGGCAGGTGCTGGTG AAGGTCAATTCTGCAGGTGATGCCATTGGCCTGCAGCCAGATGCCCGTGGTGTGGCCACATCTCTGGGGC TCAATGAGCGTCTCTTTGTTGTCAACCCACAGGAAGTGCATGAGCTGATCCCACACCCTGACCAGCTGGG GCCCACTGTGGGCTCTGCTGAGGGGCTGGACCTGGTGAGTGCCAAGGACCTGGCAGGCCAGCTGACGGAC CACGACTGGAGCCTCTTCAACAGTATCCACCAGGTGGAGCTGATCCACTATGTGCTGGGCCCCCAGCATC TGCGGGATGTCACCACCGCCAACCTGGAGCGCTTCATGCGCCGCTTCAATGAGCTGCAGTACTGGGTGGC CACCGAGCTGTGTCTCTGCCCCGTGCCCGGCCCCCGGGCCCAGCTGCTCAGGAAGTTCATTAAGCTGGCG GCCCACCTCAAGGAGCAGAAGAATCTCAATTCCTTCTTTGCCGTCATGTTTGGCCTCAGCAACTCGGCCA TCAGCCGCCTAGCCCACACCTGGGAGCGGCTGCCTCACAAAGTCCGGAAGCTGTACTCCGCCCTCGAGAG GCTGCTGGATCCCTCATGGAACCACCGGGTATACCGACTGGCCCTCGCCAAGCTCTCCCCTCCTGTCATC CCCTTCATGCCCCTTCTTCTCAAAGACATGACCTTCATTCATGAGGGAAACCACACACTAGTGGAGAATC TCATCAACTTTGAGAAGATGAGAATGATGGCCAGAGCCGCGCGGATGCTGCACCACTGCCGAAGCCACAA CCCTGTGCCTCTCTCACCACTCAGAAGCCGAGTTTCCCACCTCCACGAGGACAGCCAGGTGGCGAGGATT TCCACATGCTCGGAGCAGTCCCTGAGCACCCGGAGTCCAGCCAGCACCTGGGCTTATGTCCAGCAGCTGA AGGTCATTGACAACCAGCGGGAACTCTCCCGCCTCTCCCGAGAGCTGGAGCCATGA >OriGene 5' read for NM_006105 unedited
AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCCCCACCAC GCCCCCACCCCTCTACTCACCCAAGAGGAGCCTCTCCACCGAGCCCTGCATCCTGCTGCC CTGGCAGCCAGTCCAGCTAGGCACTGGCCCAGTTCACTGTACCTGGACTTACCCACTGGC ACCTTCCGGATCCCCTTATCAAAGCTGATGGGGGTCGCTGGGGACCCCCCAGCCTTTTGC TAGAGCCTGCACGGCTGTGCGAGCTTGAAAAGAAACATGAAGGTGGGCTGGCCAGGTGAG AGCTGCTGGCAGGTGGGCCTGCCTGTGGAGGATAGCCCAGCTCTGGGAGCACCGCGGGTG GGAGCCCTCCCTGACGTGGTGCCGGAGGGGACACTACTCAACATGGTGTTGAGAAGGATG CACCGGCCCCGAAGCTGCTCCTACCAGCTGCTGCTGGAGCACCAGCGTCCGAGCTGCATC CAGGGGCTGCGCTGGACACCACTCACCAACAGCGAGGAGTCCCTGGATTTCAGCGAGAGC CTGGAGCAGGCCTCCACAGAGCGGGTGCTCAGGGCTGGGAGGCAGCTGCATCGGCATCTG CTGGCCACCTGCCCAAACCTCATCCGAGACCGGAAGTACCACCTTANGCTCTATCGGCAG TGCTGCTCTGGCCGGGAGCTGGTGGATGGGATCTTGGCCCTGNNGACTTGGGTCCATTCC CGGACCAAGTTGTGGGAATCTGNCCAGTGCTGCTGGATGAAGTGCCCTCTGCCATGTGAA ACACGACTGGGCCTTTNCAGACCGAGATGCCCAATTCTACCGGNTCCCCGGGCCCGAGCC CGAGCCCGTGGGGACTCATGAGATGGANNGAGAGTNGNCGAANCTGTGC >OriGene 3' read for NM_006105 unedited
CCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTGGTGTGGAGACCTCTCTATTG CACACAACGTCCCAGCACACTCCACTCTCCACCCACTCCTGGGGCCTCTCTGTCCTCCCT TCCTTGGCCTTGGGTCATTCGTTATTCCTGGCTCGGGTCCACTCCGAGGTCCTCCTTAGC TGCCAGTCATCACAGGGGATGGCTGCCTCCACACTGCCTGCTCCAGGGACTCGAGTCCAC TCCACGGAGAGCCTTGCCCAGCTGTGGCCAGCCTGTGAGTATCTTGGCCCGGCACACCCT GGCTTTCCCGGCTGCAAGTGCCTGCTCCAGCTCCAGTCCCAGCCCCTCCTCATGGCTCCA GCTCTCGGGAGAGGCGGGAGAGTTCCCGCTGGTTGTCAATGACCTTCACCTGCTGGACAT AAGCCCAGGTGCTGGCTGGACTCCGGGTGCTCACGGACTGCTCCGAGCCTGTGGAAATCC TCCCCACCTGGCTGTCCTCGCGGAGGTGGGAAACTCGGCTCCTGAGTGGTGACAGAGGCA CAGGGCTGTGGCTTCGGCAGTGGTGCAACATCCGTGCGGGTCTGGCCATCATTCTCATCT TCCCACAGCCGATGAGATTCTCCACTAGCGTGTGGGTTCCCTCATGACAGAAGGGCATGC CTTTCACAACAACGGGGCCGAACGGGATGACAGGAGGCGAAAGCTCCGGCCAGGCCCANT CGGTATACCCGGGGGCTTCATGACGGACCCCACCTTCCCTTTGGGGGGGAAACAATTTCC GGCCTTTGGGCGCAATCCCTTCCACCCGCCGGTTTAGGTCGCCGTTGTCCAATTCCCCCG GCCACCCCTCCTCCGTAACAACGAATTCCTTATCTTCCGCTCCTTCGCGCGCCGCCGCCC CTTAATTTATTCCCTCCAACTCGGCCCCGTTGCGCCCCTCTGCGCCCACACCCCCTCTGC GAATCTTATCCCCCCCCCTACTTCGCTTATACCGCACCCT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_006105 |
| Insert Size | 3570 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006105.3, NP_006096.2 |
| RefSeq Size | 3327 bp |
| RefSeq ORF | 2646 bp |
| Locus ID | 10411 |
| UniProt ID | O95398 |
| Domains | DEP, cNMP, RasGEFN, RasGEF |
| Protein Pathways | Leukocyte transendothelial migration, Long-term potentiation |
| Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate 5' exon and differs in the 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream AUG and an isoform (b) with a shorter N-terminus compared to isoform a. Variants 2 and 3 encode the same isoform. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Overexpression of exchange protein directly activated by cAMP-1 (EPAC1) attenuates bladder cancer cell migration
,Ichikawa, H;Itsumi, M;Kajioka, S;Maki, T;Lee, K;Tomita, M;Yamaoka, S;,
Biochemical and Biophysical Research Communications
,PubMed ID 29111327
[RAPGEF3]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204518 | RAPGEF3 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2 |
CNY 3,990.00 |
|
| RC204518L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204518L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204518 | RAPGEF3 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2 |
CNY 4,370.00 |
|
| SC323918 | RAPGEF3 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 3 (RAPGEF3), transcript variant 2 |
CNY 2,950.00 |
