MRPS18B (NM_014046) Human Untagged Clone
CAT＃: SC115191
MRPS18B (untagged)-Human mitochondrial ribosomal protein S18B (MRPS18B), nuclear gene encoding mitochondrial protein
CNY 2400.00
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C6orf14; HSPC183; HumanS18a; MRP-S18-2; MRPS18-2; PTD017; S18amt |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC115191 sequence for NM_014046 edited (data generated by NextGen Sequencing)
ATGGCGGCGTCTGTATTAAACACCGTGCTGAGGCGGCTTCCTATGCTATCTCTCTTCCGA GGTTCTCACAGAGTTCAGGTTCCCCTCCAGACTCTTTGCACCAAAGCTCCCTCTGAGGAA GATTCTTTGTCCTCAGTTCCCATTTCTCCTTATAAGGATGAGCCCTGGAAATATCTGGAA TCAGAAGAATACCAGGAGCGATATGGTTCTCGCCCCGTCTGGGCTGACTACCGCCGCAAC CACAAGGGTGGTGTACCCCCACAGCGGACTCGGAAGACATGTATTCGTCGGAATAAAGTT GTTGGGAATCCCTGCCCCATCTGTCGAGATCACAAGTTGCATGTTGACTTTAGGAACGTG AAGCTCTTGGAGCAATTTGTCTGCGCCCACACGGGTATCATCTTCTATGCTCCATACACA GGAGTCTGTGTGAAGCAGCACAAGCGGTTGACCCAGGCCATCCAGAAAGCCAGGGATCAT GGTCTCCTCATTTACCACATCCCCCAGGTTGAACCACGGGACCTTGACTTCAGTACCTCT CATGGGGCTGTGAGTGCTACTCCGCCAGCCCCCACCCTGGTCTCAGGTGACCCCTGGTAC CCATGGTACAACTGGAAACAGCCACCGGAGAGAGAACTGTCTCGCCTTCGCCGGCTTTAC CAGGGTCATCTCCAAGAAGAGAGTGGCCCCCCACCTGAGTCAATGCCCAAGATGCCCCCT AGAACACCAGCGGAAGCCTCCTCCACTGGGCAGACAGGCCCTCAGAGTGCTCTGTAG Clone variation with respect to NM_014046.3 >OriGene 5' read for NM_014046 unedited
GTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGGTCAAGATGGCGGCGTC TGTATTAAACACCGTGCTGAGGCGGCTTCCTATGCTATCTCTCTTCCGAGGTTCTCACAG AGTTCAGGTTCCCCTCCAGACTCTTTGCACCAAAGCTCCCTCTGAGGAAGATTCTTTGTC CTCAGTTCCCATTTCTCCTTATAAGGATGAGCCCTGGAAATATCTGGAATCAGAAGAATA CCAGGAGCGATATGGNTCTCGCCCCGNCTGGGCTGACTACCGCCGCNNCCNCNNGGGGGG NGNNCCCCCNCNGCGGNCTCGGAAGACATGTATTCGTCGGAATAAAGTTGTTGGGAATCC CTGCCCCATCTGTCGAGATCACAAGTTGCATGTTGACTTTAGGAACGTGAAGCTCTTGGA GCAATTTGTCTGCGCCCACACGGGTATCATCTTCTATGCTCCATACACAGGAGTCTGTGT GAAGCAGCACAAGCGGTTGACCCAGGCCATCCAGAAAGCCAGGGATCATGGTCTCCTCAT TTACCACATCCCCCAGGTTGAACCACGGGACCTTGACTTCAGTACCTCTCATGGGGCTGT GAGTGCTACTCCGCCAGCCCCCACCCTGGTCTCAGGTGACCCCTGGTACCCATGGTACAA CTGGAAACAGCCACCGGAGAGAGAACTGTCTCGCCTTCGCCGGCTTTACCAGGGTCATCT CCAAGAAGAGAGTGGCCCCCCACCTGAGTCAATGCCCAAGATGCCCCCTAGAACACCAGC GGNAGCCTCCTCCCTGGGCAGACAGGCCCTCAGAGTGCTCTGTNAGAGCTGTAAACTGGG AAGAGAGGCCAGCGTGGGGCTCACTCTGATCACTTTTGGGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014046 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014046.2, NP_054765.1 |
| RefSeq Size | 1439 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 28973 |
| UniProt ID | Q9Y676 |
| Domains | Ribosomal_S18 |
| Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Jul 2008] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202795 | MRPS18B (Myc-DDK-tagged)-Human mitochondrial ribosomal protein S18B (MRPS18B), nuclear gene encoding mitochondrial protein |
CNY 2400.00 |
|
| RC202795L3 | Lenti ORF clone of Human mitochondrial ribosomal protein S18B (MRPS18B), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202795L4 | Lenti ORF clone of Human mitochondrial ribosomal protein S18B (MRPS18B), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5890.00 |
|
| RG202795 | MRPS18B (tGFP-tagged) - Human mitochondrial ribosomal protein S18B (MRPS18B), nuclear gene encoding mitochondrial protein |
CNY 4000.00 |
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