ARFGAP3 (NM_014570) Human Untagged Clone
CAT＃: SC114939
ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1
CNY 3848.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARFGAP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114939 sequence for NM_014570 edited (data generated by NextGen Sequencing)
ATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTTCAAGCGCCTCCGCTCGGTGCCC ACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCCCAGCTGGGCAAGCATAACCTAT GGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCGGTCACTTGGTGTTCACTTGAGT TTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATGGTTTCAGTTGCGATGCATGCAA GTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCAACATGGGTGTTCCACCAATGAC ACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTATAGGGAGAAAATCAAATCGCTC GCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTGGCTTGATAGTTGTGTGGTTCCA CCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGCCTCTCACGTTTCTCCTGAGGTG AGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATCTTCTTTAACATCAAGGCCTGTG GAAACCACTTTGGAAAATAATGAAGGTGGACAAGAGCAAGGACCAAGTGTGGAAGGTCTT AATGTACCAACAAAGGCTACTTTAGAGGTATCCTCTATCATAAAAAAGAAACCAAATCAA GCTAAAAAAGGCCTTGGGGCCAAAAAAGGAAGTTTGGGAGCTCAGAAACTGGCAAACACA TGCTTTAATGAAATTGAAAAACAAGCTCAAGCTGCGGATAAAATGAAGGAGCAGGAAGAC CTGGCCAAGGTGGTATCTAAAGAAGAATCAATTGTTTCATCATTACGATTAGCCTATAAG GATCTTGAAATTCAAATGAAGAAAGACGAAAAGATGAACATTAGTGGCAAAAAAAATGTT GACTCAGACAGACTCGGCATGGGATTTGGAAATTGCAGAAGTGTTATTTCACATTCAGTG ACTTCAGATATGCAGACCATAGAGCAGGAATCACCCATTATGGCAAAACCAAGAAAAAAG TATAATGATGACAGTGACGATTCATATTTTACTTCCAGCTCAAGTTACTTTGACGAGCCA GTGGAGTTAAGGAGCAGTTCTTTCTCTAGCTGGGATGACAGTTCAGATTCCTATTGGAAA AAAGAGACCAGCAAAGATACTGAAACAGTTCTGAAAACCACAGGCTATTCAGACAGACCT ACTGCTCGCCGCAAGCCAGATTATGAGCCAGTTGAAAATACAGATGAGGCCCAGAAGAAG TTTGGCAATGTCAAGGCCATTTCATCAGATATGTATTTTGGAAGACAATCCCAGGCTGAT TATGAGACCAGGGCCCGCCTAGAGAGGCTGTCGGCAAGTTCCTCCATAAGCTCGGCTGAT CTGTTCGAGGAGCCGAGGAAGCAGCCAGCAGGGAACTACAGCCTGTCCAGTGTGCTGCCC AACGCCCCCGACATGGCGCAGTTCAAGCAGGGAGTGAGATCGGTTGCTGGAAAACTCTCC GTCTTTGCTAATGGAGTCGTGACTTCAATTCAGGATCGCTACGGTTCTTAA Clone variation with respect to NM_014570.4 >OriGene 5' read for NM_014570 unedited
TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGC ACGAGGCTGCTGAGGCGGTAGCCGCTTTTCGTCGACTCTTACCGGTTGGCTGGGCCAGCT GCGCCGCGGCTCACAGCTGACGATGGGGGACCCCAGCAAGCAGGACATCTTGACCATCTT CAAGCGCCTCCGCTCGGTGCCCACTAACAAGGTGTGTTTTGATTGTGGTGCCAAAAATCC CAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGATTGCTCAGGGTCCCACCG GTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAACTGGTCATG GTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTTTTCATCA ACATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTCAGCTCTA TAGGGAGAAAATCAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTGATCTGTG GCTTGATAGTTGTGTGGTTCCACCTTTGTCCCCTCCACCAAAGGAGGAAGATTTTTTTGC CTCTCACGTTTCTCCTGAGGTGAGTGACACAGCGTGGGCATCAGCAATAGCAGAACCATC TTCTTTACATCAAGGCCTGTGGAAACCACTTTGGAAAATAATGAAGGTGGACAAAGAGCA GGACCAAGTGTGGAAGGTCTTAATGTACCAACANAGGCTACTNTAGAGGTATCCTCTATC ATAAAAAGAACCAAATCAGCTAAAAAAGGCCTTGGGCCAAAAAGGAAAGTTGGAGCTCAG AACTGGCAACACATGCCTTAATGAATTGAAAACANGCTCAGCTGCGGATAAATGAANGAG CAGAGACTGNCCAGGTGGTATCTAAGAAAC >OriGene 3' read for NM_014570 unedited
GGCGCAGGCGGNGNNGNCCCTCACCCCCAGATTTGCCGTGTTTTTCTTTTATAATCTCAA ATTNGACTTTTAAAAAACATCACAAAATTTCATTCAAAACCACATTTTTGGCTTATCAAA TTTCAAATATATTTTACTGTGCNTGAACAATATATTCTAATGCTGTCTAAAACACAGCTA AATTATTTTTCTTTATTTGTTTATACACATTCGGTAATTTCTGAAAAGCAAGATTTAAAA ATATTTATTAACAAAACTACCCAATTACAATGACTGTTCTCCCATACACGCAACTATTTT CTGTAGCTGTATCTTCTTACCTCATTCCACTTTAACTCTGTATACCGTATTGATTTGTGA TGAGATGATTTATTATGAGAACTCTTAGGGAGTTCTCATCTTCCATTTCTCATCAATTCA AACAGCAACACCTTTCACAAGATAACATTAATTCCCTTGGCAGGGCAGAAGCTTAAGTTT GTTAAAAGCACTCACTGAAAAACATTTTTAAATTTATAGGTCATATAAAATAATTTACAA AGAGACAGATGACTTCAAATATTATTTGGCAGTCACCTTACTATGTAGAAACATAAATGA AGCAATCTGTCACACGAGACACCAGTCACTTTTGGTCTTTTGAAGGAAGTGTGTGTGGTG AGTGTTGTTTCTGCAGGCCCATCCAGCATTCGCTGATTCTGGCAGCCCTGAGCCCATGTG TCACATGGAGACCTCTTCTTCCTCCCCACATGAAGCCTCCTGGTTCAAGAGCANGAGGAG CCCGAGTCTCCAGGCCCTCATGTTGAAATCAAAGTTCCAGGAAATAAACCAGGATTTCCC GAAGCGCCCAAGAAGGTGTGACAGGAAGAACGTGAACCAATTCACAGAAAGCTCCTACTC AAACTCAAATTTTCAAAGAAATTTAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014570 |
| Insert Size | 2910 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014570.3, NP_055385.2 |
| RefSeq Size | 2725 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 26286 |
| UniProt ID | Q9NP61 |
| Domains | ArfGap |
| Protein Pathways | Endocytosis |
| Gene Summary | The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202392 | ARFGAP3 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3840.00 |
|
| RC202392L3 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202392L4 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG202392 | ARFGAP3 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 5440.00 |
|
| SC324381 | ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3848.00 |
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