KLHL21 (NM_014851) Human Untagged Clone
CAT＃: SC114808
KLHL21 (untagged)-Human kelch-like 21 (Drosophila) (KLHL21)
CNY 4456.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114808 sequence for NM_014851 edited (data generated by NextGen Sequencing)
ATGGAGCGACCGGCGCCCCTGGCCGTGCTTCCCTTCTCGGACCCCGCGCACGCCCTGAGC CTGCTGCGCGGCCTGAGCCAGCTGCGCGCCGAGCGCAAGTTCCTGGACGTGACCCTGGAG GCGGCGGGCGGGCGCGACTTCCCGGCGCACCGTGCGGTGCTGGCCGCCGCCAGCCCCTAC TTCCGCGCCATGTTCGCGGGGCAGCTGCGCGAGAGCCGCGCCGAGCGGGTGCGCCTGCAC GGAGTGCCTCCCGACATGCTGCAGCTGCTGCTGGACTTCAGCTACACGGGCCGCGTGGCG GTAAGCGGCGACAACGCTGAGCCGCTGCTGCGCGCCGCCGACCTGCTGCAGTTCCCGGCC GTGAAGGAGGCGTGCGGGGCCTTCCTGCAGCAGCAGCTCGACCTGGCCAACTGCCTGGAC ATGCAGGACTTCGCTGAGGCCTTCAGCTGCTCGGGACTGGCGAGCGCGGCGCAGCGGTTC ATTCTGCGCCACGTGGGCGAGCTGGGCGCCGAGCAGCTGGAGCGGCTGCCACTGGCGCGC CTGCTGCGCTACCTGCGGGACGACGGGCTGTGTGTGCCCAAGGAGGAGGCCGCCTACCAG CTGGCGCTGCGCTGGGTCCGCGCTGACCCGCCGCGCCGCGCCGCGCACTGGCCGCAGCTG CTGGAGGCCGTGCGCCTGCCCTTCGTGCGCCGCTTCTACCTGTTGGCGCACGTCGAGGCC GAGCCGCTGGTGGCGCGCTGCCCACCCTGCCTGCGCCTGCTGCGCGAGGCGCGCGACTTC CAGGCGGCGCGCTACGACCGCCACGACCGCGGGCCCTGTCCCCGAATGCGTCCTCGCCCG TCCACCGGTCTCGCCGAGATCCTCGTGCTCGTGGGCGGCTGCGACCAGGACTGTGACGAG CTGGTCACTGTCGACTGCTACAACCCGCAGACGGGTCAGTGGCGCTACCTGGCCGAGTTC CCAGACCACCTGGGCGGAGGCTACAGCATCGTGGCGCTGGGCAATGACATCTACGTGACG GGTGGGTCCGATGGCTCCCGGCTCTATGACTGCGTGTGGAGGTACAACTCAAGCGTGAAT GAGTGGGCGGAGGTGGCGCCCATGCTGAAGGCCCGCGAGTACCACAGCTCCTCTGTGCTG GACGGACTGCTGTACGTGGTGGCCGCCGACAGCACCGAGCGCTATGACCACACCACTGAC TCCTGGGAGGCCCTGCAGCCCATGACCTACCCCATGGACAACTGCTCCACCACTGCGTGC CGTGGCCGGCTCTATGCCATCGGCTCCCTGGCTGGCAAGGAGACCATGGTGATGCAGTGC TACGACCCGGACACCGACCTGTGGTCGCTGGTGGACTGCGGCCAGCTCCCGCCCTGGTCC TTCGCCCCCAAGACTGCGACTCTAAACGGACTCATGTACTTTGTCAGGGATGACTCCGCT GAGGTGGACGTGTACAACCCGACGAGGAACGAATGGGACAAGATCCCGTCCATGAATCAG GTACATGTGGGGGGCAGCCTGGCCGTCCTTGGGGGGAAGCTGTACGTCTCTGGGGGATAC GACAATACATTTGAACTCTCGGACGTGGTAGAGGCCTATGACCCAGAGACTCGCGCGTGG AGCGTGGTGGGGCGGCTCCCAGAACCCACCTTCTGGCATGGCAGTGTCAGCATCTTCCGC CAGTTCATGCCCCAGACCTTCTCGGGTGGGCGTGGCTTCGAGTTGGACAGTGGCAGCGAT GACATGGACCCAGGCCGACCCCGGCCGCCGCGGGACCCCGATGAGCTGCACTAG Clone variation with respect to NM_014851.2 1029 t=>c >OriGene 5' read for NM_014851 unedited
GCATTTGTATACGACTACTATAGGCGGCCCGCGAATTCGCACGAGGCTAGCGCGCCGCGT CTCTCCGCAGGCCGCGGCCGCGGCGTCCTCGACAACCTATCGAAGGCGCCATGGAGCGAC CGGCGCCCCTGGCCGTGCTTCCCTTCTCGGACCCCGCGCACGCCCTGAGCCTGCTGCGCG GCCTGAGCCAGCTGCGCGCCGAGCGCAAGTTCCTGGACGTGACCCTGGAGGCGGCGGGCG GGCGCGACTTCCCGGCGCACCGTGCGGTGCTGGCCGCCGCCAGCCCCTACTTCCGCGCCA TGTTCGCGGGGCAGCTGCGCGAGAGCCGCGCCGAGCGGGTGCGCCTGCACGGAGTGCCTC CCGACATGCTGCAGCTGCTGCTGGACTTCAGCTACACGGGCCGCGTGGCGGTAAGCGGCG ACAACGCTGAGCCGCTGCTGCGCGCCGCCGACCTGCTGCAGTTCCCGGCCGTGAAGGAGG CGTGCGGNGCCTTCCTGCAGCAGCAGCTCGACCTGGCCAACTGCCTGGACATGCAGGACT TCGCTGAGGCCTTCAGCTGCTCGGGACTGGCGAGCGCGGCGCAGCGGTTCATTCTGCGCC ACGTGGGCGAGCTGGGCGCCGAGCAGCTGGAGCGGCTGCCACTGGCGCGCCTGCTGCGCT ACCTGCGGGACGACGGGCTGTGTGTGCCCAGGGAGAGGCCGCCTACCAGCTGGCGCTGCG CTGGGTCCGCGCTGACCCGCCGNGCCGCGCCGCGCACTGGCCGCAGCTGCTTGGAGGCCG TGCGCCTGCCCTTCGTGCGCCGCTTCTACCTGTTGGCGCACGTCNAGGCCGAGCCGCTGG TGGNCGCGCTGCCCACCCTGCCTGCGCCTGCTGCGCGAGGCGCGCGACTTNCAGGCGGNG CGCTACGACGCCGNNACGCGGGCCTGTNCCCCGAATGCGTCTCGCCGTCCACGTCTTTGC CGGGATCTCGGGCT >OriGene 3' read for NM_014851 unedited
GACGCGGCCGCACTCTATGATCGAGTTTTTTTTTTTTTTTTTTGGATAGGAAATACTTTT ATTGCAAAAAGTACTGAAGGTACCCTAAAACCTTAAGCAGGATTCTGGACATGGAAGCCT ACAGAATAGACAAAAATAAATATGTCAACCTGCCCGACCCTCTGGGGTGAACTGGATGTG GACACTGGAGGGAGGGCGTCCTTTATTACATACGCGTCTCTGAAGTCATATAAATATAGA ATACCTTATAGTAGATCAGCATTAAATACCAGTCACTGTGTTTATATAACTTAATTTGAG CTGTAGACAAAGTTCTGTACATGTAACATGTGGCCATGCCCAGGCATCCCAGCATCTATC CTGAAGTCAGTGTAAAGACATCCTTAAGTGGTGGAGACATGACAGCCCAGAACCCACAGC AGAGGGAAACTGGGGAGAGGAGGGGGCCTGACCCTGGATCCCCCAGCTGGTGTCCACAGG CTAGAGGGACCCATTGCTGCAGAAGCTGGTGCCTGGTTTTCCCCATACTTGGTCTTCTAA ACCCAAAGACAGGGTCCTGATGGTGGAGACCTGGGTAGGTCCCGAGGACAGACACAGGAG AGGGCACAATCCCAAGCGCAGCTCTCCTGCACTGGCTCGCACCACAAGCCTCATCTGAGT GGCCGACACAAGCCTGGAGTACCGCAGCTGCCAACCACAGCAAAGCCGCAAAAAGACTGA GAAAACAAGGATCCCAGATTTCCACAAGGGCAAGGCCTCGCATACCATCCAAGCCCGAGA TGCCCTGTGCCTCCAGTCATCACATTCTGGACAGATGTTCCTCTCGTGGTGCCCACCTCA CTGCCTTGGAACGTCAGGGATTGAGGCCGTAACAACCCTTTATGCCAAACCAGCCTTAAC CCCCTGCTGAAAGGAGAAAAGCTGCCCCGCTAACTCCACAACCTTTGGTGGGCTAACACC CCCTAGATTTAAGGAACCCCGGCGCGGGGCCTCCTGAGATTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014851 |
| Insert Size | 4470 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014851.2, NP_055666.2 |
| RefSeq Size | 4502 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 9903 |
| UniProt ID | Q9UJP4 |
| Domains | BTB, Kelch |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205352 | KLHL21 (Myc-DDK-tagged)-Human kelch-like 21 (Drosophila) (KLHL21) |
CNY 4448.00 |
|
| RC205352L3 | Lenti ORF clone of Human kelch-like 21 (Drosophila) (KLHL21), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205352L4 | Lenti ORF clone of Human kelch-like 21 (Drosophila) (KLHL21), mGFP tagged |
CNY 6848.00 |
|
| RG205352 | KLHL21 (tGFP-tagged) - Human kelch-like 21 (Drosophila) (KLHL21) |
CNY 4370.00 |
