NOP53 (NM_015710) Human Untagged Clone
CAT＃: SC114619
GLTSCR2 (untagged)-Human glioma tumor suppressor candidate region gene 2 (GLTSCR2)
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GLTSCR2; PICT-1; PICT1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114619 sequence for NM_015710 edited (data generated by NextGen Sequencing)
ATGGCGGCAGGAGGCAGTGGCGTTGGTGGGAAGCGCAGCTCGAAAAGCGATGCCGATTCT GGTTTCCTGGGGCTGCGGCCCACTTCGGTGGACCCAGCGCTGAGGCGGCGGCGGCGAGGC CCAAGAAATAAGAAGCGGGGCTGGCGGCGGCTTGCTCAGGAGCCGCTGGGGCTGGAGGTT GACCAGTTCCTGGAAGACGTGCGGCTACAGGAGCGCACGAGCGGTGGCTTGTTGTCAGAG GCCCCAAATGAAAAACTCTTCTTCGTGGACACTGGCTCCAAGGAAAAAGGGCTGACAAAG AAGAGAACCAAAGTCCAGAAGAAGTCACTGCTTCTCAAGAAACCCCTTCGGGTTGACCTC ATCCTCGAGAACACATCCAAAGTCCCTGCCCCCAAAGACGTCCTCGCCCACCAGGTCCCC AACGCCAAGAAGCTCAGGCGGAAGGAGCAGCTATGGGAGAAGCTGGCCAAGCAGGGCGAG CTGCCCCGGGAGGTGCGCAGGGCCCAGGCCCGGCTCCTCAACCCTTCTGCAACAAGGGCC AAGCCCGGGCCCCAGGACACCGTAGAGCGGCCCTTCTACGACCTCTGGGCCTCAGACAAC CCCCTGGACAGGCCGTTGGTTGGCCAGGATGAGTTTTTCCTGGAGCAGACCAAGAAGAAA GGAGTGAAGCGGCCAGCACGCCTGCACACCAAGCCGTCCCAGGCACCCGCCGTGGAGGTG GCGCCTGCCGGAGCTTCCTACAATCCATCCTTTGAAGACCACCAGACCCTGCTCTCAGCG GCCCACGAGGTGGAGTTGCAGCGGCAGAAGGAGGCGGAGAAGCTGGAGCGGCAGCTGGCC CTGCCCGCCACGGAGCAGGCCGCCACCCAGGAGTCCACATTCCAGGAGCTGTGCGAGGGG CTGCTGGAGGAGTCGGATGGTGAGGGGGAGCCAGGCCAGGGCGAGGGGCCGGAGGCTGGG GATGCCGAGGTCTGTCCCACGCCCGCCCGCCTGGCCACCACAGAGAAGAAGACGGAGCAG CAGCGGCGGCGGGAGAAGGCTGTGCACAGGCTGCGGGTACAGCAGGCCGCGTTGCGGGCC GCCCGGCTCCGGCACCAGGAGCTGTTCCGGCTGCGCGGGATCAAGGCCCAGGTGGCCCTG AGGCTGGCGGAGCTGGCGCGGCGGCAGAGGCGGCGGCAGGCGCGGCGGGAGGCTGAGGCT GACAAGCCCCGAAGGCTGGGGCGGCTCAAGTACCAGGCACCTGACATCGACGTGCAGCTG AGCTCGGAGCTGACAGACTCGCTCAGGACCCTGAAGCCCGAGGGCAACATCCTTCGAGAC CGGTTCAAGAGCTTCCAGAGGAGGAATATGATCGAGCCTCGAGAGAGAGCCAAGTTCAAA CGCAAGTACAAGGTGAAGCTGGTGGAGAAGCGGGCGTTCCGTGAGATCCAGTTGTAG Clone variation with respect to NM_015710.4 >OriGene 5' read for NM_015710 unedited
TCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAGNAGAACTAG TCTCGAGTTTTTTTTTTTTTTTTTTTTGACAAGATGGCGGCAGGAGGCAGTGGCGTTGGT GGGAAGCGCAGCTCGAAAAGCGATGCCGATTCTGGTTTCCTGGGGCTGCGGCCCACTTCG GTGGACCCAGCGCTGAGGCGGCGGCGGCGAGGCCCAAGAAATAAGAAGCGGGGCTGGCGG CGGCTTGCTCAGGAGCCGCTGGGGCTGGAGGTTGACCAGTTCCTGGAAGACGTGCGGCTA CAGGAGCGCACGAGCGGTGGCTTGTTGTCAGAGGCCCCAAATGAAAAACTCTTCTTCGTG GACACTGGCTCCAAGGAAAAAGGGCTGACAAAGAAGAGAACCAAAGTCCAGAAGAAGTCA CTGCTTCTCAAGAAACCCCTTCGGGTTGACCTCATCCTCGAGAACACATCCAAAGTCCCT GCCCCCAAAGACGTCCTCGCCCACCAGGTCCCCAACGCCAAGAAGCTCAGGCGGAAGGAG CAGCTATGGGAGAAGCTGGCCAAGCAGGGCGAGCTGCCCCGGGAGGTGCGCAGGGCCCAG GCCCGGCTCCTCAACCCTTCTGCAAAAGGGCCAAGCCCGGGCCCCAGGACACCGTAGAGC GGCCCTTCTACGACCTCTGGGCCTCAGACAACCCCCTGGACAGGCCGTTGGTTGGCCAGG ATGAGTTTTTCCTGGAGCAGACCAAGAAGAAAGGAGTGAAGCGGCCAGCACGCCTGCACA CCAAGCCGTCCCAGGCACCCGCCGTGGGAGTGGCGCCTGCCGNAGCTTCCTACAATCCAT CCCTTTGAAGACACCAGAACCTGCTCTCAGCGGCCCACNAGGTGGAGTTGCAACGCANAA GGAGGCGAAAACTGGAGCGCAGCTGGCCTGCCCGCACGNACAGCCGCCCCCCAGATCCAC ATCCAGGG >OriGene 3' read for NM_015710 unedited
ATATGATGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACAACTAAAAAAAATTTTTTATTGAA GGGGGAATCTCCGGCATTTGATGGGAGCTACAACTGGGTTTTAAGGAACCCCCGCTTTTC CACCAACTTTACCTTGGACTTGCGTTTGAACTTGGCTCTTTCTCGAGGCTCGATCAAATT CCCCCCCTGGAAACTCTTGAACCCGTCTCAAAAGAAGTTGCCCCCGGGCTTTAAGGGCCT GAACGAATTTGTCAACTCCCAACTCAACTGCACCTTAATGTCAGGGGCCCGGGACTTGAA CCCCCCCAACCTTTGGGGCTTGGTAACCTTAAACTTCCGCCGGGCCTGGCGCCGCCTTTG GCGCCGGGCCAGCTTCGCCAACCTTAGGGCCACCTGGGCCTTGATCCCCCCCAACCGGAA CAACTCCTGGTGCCCGAACCGGGCGGCCCCCAACGCGGCCTGGTGTACCCGCAACCTGTG CACAACCTTTTTCCGCCGCCGCTGGTGGTCCGTTTTTTTTTTTGGGGGGGCCAAGCCGGC GGGCGTGGGACAAAACTTGGGATTCCCAGCCTCCGGGCCCTCGCCCTGGGCTGGGTTCCC CTTAACATTCGAATTCTTCAGAAGCCCCTTGCACAAGTTCTGGAATGGGGACTCCTGGGT GGCGGCCTGCTCCCTGGCGGGCAGGGCCAAATGGCGNTCCAAATTTTTCGCCTCTTTTGG CGGTTGAACTCCACCTNTGGGCCCCTGAAAACAAGTTCTGGGGGCCTTCAAAGGATGGAT TGAAGAAACTCCGGAGGGCCACCTCCACGGGGGTGCCTGGGACGCTTTGTGTGCAACCTT C |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015710 |
| Insert Size | 1750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015710.3, NP_056525.1 |
| RefSeq Size | 1567 bp |
| RefSeq ORF | 1437 bp |
| Locus ID | 29997 |
| UniProt ID | Q9NZM5 |
| Protein Families | Druggable Genome |
| Gene Summary | Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis (PubMed:24120868). In ribosome biogenesis, may also play a role in rRNA transcription (PubMed:27729611). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions (PubMed:21741933, PubMed:24120868, PubMed:27829214). DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:24120868, PubMed:27829214). It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation (PubMed:22522597). Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:15355975, PubMed:16971513, PubMed:27729611). May also inhibit cell proliferation and increase apoptosis through its interaction with NF2 (PubMed:21167305). May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation (PubMed:25818168). Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex (PubMed:25956029). May also regulate cellular aerobic respiration (PubMed:24556985). In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of DDX58/RIG-1 (PubMed:27824081).[UniProtKB/Swiss-Prot Function] |
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Other Versions
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| RC204035L3 | Lenti ORF clone of Human glioma tumor suppressor candidate region gene 2 (GLTSCR2), Myc-DDK-tagged |
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| RC204035L4 | Lenti ORF clone of Human glioma tumor suppressor candidate region gene 2 (GLTSCR2), mGFP tagged |
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| RG204035 | GLTSCR2 (tGFP-tagged) - Human glioma tumor suppressor candidate region gene 2 (GLTSCR2) |
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