IFT52 (NM_016004) Human Untagged Clone
CAT＃: SC114489
IFT52 (untagged)-Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52)
CNY 3656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C20orf9; CGI-53; NGD2; NGD5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114489 sequence for NM_016004 edited (data generated by NextGen Sequencing)
ATGGAGAAAGAGCTGCGGAGCACCATTCTTTTCAATGCCTACAAAAAGGAGATATTTACC ACCAACAATGGCTACAAATCCATGCAGAAAAAACTTCGGAGTAATTGGAAGATTCAGAGC TTAAAAGATGAAATCACATCTGAGAAGTTAAATGGAGTAAAACTGTGGATTACAGCTGGG CCAAGGGAAAAATTTACTGCAGCTGAGTTTGAAATCCTGAAGAAATATCTTGACACTGGT GGAGATGTCTTTGTGATGCTAGGAGAAGGTGGAGAATCCAGATTTGACACCAATATTAAC TTTTTACTAGAAGAATATGGAATCATGGTTAATAATGATGCTGTGGTTAGAAATGTATAT CACAAATATTTCCATCCTAAAGAAGCTCTAGTTTCCAGTGGAGTCTTGAACAGGGAAATT AGCCGAGCTGCAGGAAAGGCTGTGCCTGGGATCATTGATGAGGAAAGCAGTGGAAACAAT GCCCAGGCTCTCACCTTTGTGTATCCTTTTGGTGCCACATTGAGTGTCATGAAACCAGCA GTGGCGGTTCTGTCTACAGGTTCTGTCTGCTTCCCACTTAACAGACCCATTTTGGCTTTC TATCACTCAAAGAACCAAGGTGGGAAGCTGGCAGTGCTTGGTTCATGTCACATGTTCAGT GATCAATATTTGGACAAAGAAGAAAACAGCAAAATCATGGATGTTGTTTTCCAGTGGCTC ACGACAGGAGACATCCACCTAAACCAGATTGATGCTGAGGACCCAGAGATTTCTGACTAC ATGATGCTGCCCTACACAGCCACCCTATCAAAGCGGAATCGAGAGTGTCTCCAGGAGAGT GATGAGATCCCAAGGGACTTTACCACCCTCTTCGACCTGTCCATCTTCCAGCTGGATACC ACCTCCTTCCACAGCGTCATCGAGGCTCACGAGCAGCTAAATGTGAAACATGAACCACTC CAGCTCATCCAGCCTCAGTTTGAGACGCCGCTGCCAACCCTTCAGCCTGCGGTTTTTCCT CCCAGTTTCCGGGAGTTACCACCTCCTCCTCTGGAGCTATTTGATTTAGATGAAACGTTC TCCTCTGAGAAGGCACGGCTGGCTCAGATTACCAATAAGTGTACTGAAGAAGACCTGGAA TTTTATGTCAGGAAGTGTGGTGATATTCTTGGAGTAACCAGTAAACTACCAAAGGACCAA CAGGATGCCAAACATATCCTTGAGCACGTCTTCTTCCAAGTGGTGGAGTTCAAGAAATTG AACCAGGAACATGACATCGATACAAGTGAAACAGCATTCCAGAACAATTTCTGA Clone variation with respect to NM_016004.2 >OriGene 5' read for NM_016004 unedited
CACATTTGTAAACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGCGCCTCCC GGCGCACCGACGCGCCTCTCCGGTTACTAAGCGGCCTTGGATACCTGGCCGCGGGATGCT GGGCGGCGTCAGGTGAGCGGTGGTCGCTGGGCCTCAGGTAACCATGGAGAAAGAGCTGCG GAGCACCATTCTTTTCAATGCCTACAAAAAGGAGATATTTACCACCAACAATGGCTACAA ATCCATGCAGAAAAAACTTCGGAGTAATTGGAAGATTCAGAGCTTAAAAGATGAAATCAC ATCTGAGAAGTTAAATGGAGTAAAACTGTGGATTACAGCTGGGCCAAGGGAAAAATTTAC TGCAGCTGAGTTTGAAATCCTGAAGAAATATCTTGACACTGGTGGAGATGTCTTTGTGAT GCTAGGAGAAGGTGGAGAATCCAGATTTGACACCAATATTAACTTTTTACTAGAAGAATA TGGAATCATGGTTAATAATGATGCTGTGGTTAGAAATGTATATCACAAATATTTCCATCC TAAAGAAGCTCTAGTTTCCAGTGGAGTCTTGAACAGGGAAATTAGCCGAGCTGCAGGAAA GGCTGTGCCTGNGATCATTGATGAGGAAAGCAGTGGAAACAATGCCCAGGCTCTCACCTT TGTGTATCCCTTTTGGTGCCACATTGAGTGTCATGAAACCAGCAGTGGCGGNTCTGTCTA CAGGTTCTGTCTGCTTCCCACTTACAGACCCATTCTGCTTTCTATCACTCAAAGACCCAG GTTGGGAACCTGCCAATGCTTGTTTACATGCACATGTTCACTGATCAATATTTTGGCCAA GAGAAAACACCCAATCATGGATGTTGTTTTCAGTGCCTCCCGAAAGAGAACATCCCCTCA ACCAAATTGAGCCTGAGACCCCAAAATT >OriGene 3' read for NM_016004 unedited
ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTAGGGGATAAAAAAACAAATGCCACCATTCTTTTAATTT TTAAAAATGGAATGTTTTTTACATAAGGGTTTTGGGAAAAATCTTATACCTATTTGAGTT TTACAAAAAATGCCTTTCCCTTCCAGAGCTCATGGAGGAAAAAATGTTTAAACAATTTTG ATAAGGAGTTGAAAAACCATTTGAAAATAGTTTACAAAAAGAGAATCAGGGGGCCGAAAA AGCTTTAAGAGGCATGGGTTTTAAAAATTGTGCGGGAATGGTGCTTCACTTCTATTGACG CCATGTTCCTGGTTAAATTTCTGGAACTCCCCCCCTTGGAAAAAAAACTGCTCTAAGGTT TGTCCGGCATCCCTGGTGGTCCTTTGTGAATCTTACCTGGGCACCTCAAAAATTTTCCCC CCCTTCCCGACCTAAAAACTCCAGGCTTCTTTAAGACCCCTATTGGGAACTCGAACCCAC CCGCCCTCTTCAAAGGGAAACCTCCTATTAAATTAAAATCTCCCCAGGAGGAGGGGGAAT CTCCGGAAATTGGGAGGAGAACCCCGTGGCTTCCAGGGGTGTTTCGTCTCTTTCACACTG CTGGGCCCGAAAAGCCCGCGACGTCTAATGCTCCACTATCAGGTCCCGGCGGCCCTCTCA TAACGCTGGGGAAGGTGTGGCTTCATACCAGAAAATTAACACGTTCCATACGTGTGTTAC ATTCCTTTTGGTTTTTACCCCTTTTTGTAACTCCTCTTCTCACCTCCTTGTAGGGCGGTG GTTCATCGAACAACCACTCACCACAAAATTCTTGGCCCCCCCACAAATCGGCGCACGCGG GTGTCCCGTGCCGACCCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016004 |
| Insert Size | 1720 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016004.2, NP_057088.2 |
| RefSeq Size | 1675 bp |
| RefSeq ORF | 1314 bp |
| Locus ID | 51098 |
| UniProt ID | Q9Y366 |
| Gene Summary | This gene encodes a conserved proline-rich protein that is a component of the intraflagellar transport-B (IFT-B) core complex. The encoded protein is essential for the integrity of the IFT-B core complex, and for biosynthesis and maintenance of cilia. Mutations in this gene are associated with ciliopathy that affects the skeleton. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208044 | IFT52 (Myc-DDK-tagged)-Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52) |
CNY 3656.00 |
|
| RC208044L1 | Lenti ORF clone of Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52), Myc-DDK-tagged |
CNY 6056.00 |
|
| RC208044L2 | Lenti ORF clone of Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52), mGFP tagged |
CNY 5890.00 |
|
| RC208044L3 | Lenti ORF clone of Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC208044L4 | Lenti ORF clone of Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52), mGFP tagged |
CNY 5890.00 |
|
| RG208044 | IFT52 (tGFP-tagged) - Human intraflagellar transport 52 homolog (Chlamydomonas) (IFT52) |
CNY 5256.00 |
