IF3EI (EIF3L) (NM_016091) Human Untagged Clone
CAT＃: SC114447
EIF3L (untagged)-Human eukaryotic translation initiation factor 3, subunit L (EIF3L)
CNY 4208.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF3EIP; EIF3S6IP; EIF3S11; HSPC021; HSPC025; MSTP005 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114447 sequence for NM_016091 edited (data generated by NextGen Sequencing)
ATGTCTTATCCCGCTGATGATTATGAGTCTGAGGCGGCTTATGACCCCTACGCTTATCCC AGCGACTATGATATGCACACAGGAGATCCAAAGCAGGACCTTGCTTATGAACGTCAGTAT GAACAGCAAACCTATCAGGTGATCCCTGAGGTGATCAAAAACTTCATCCAGTATTTCCAC AAAACTGTCTCAGATTTGATTGACCAGAAAGTGTATGAGCTACAGGCCAGTCGTGTCTCC AGTGATGTCATTGACCAGAAGGTGTATGAGATCCAGGACATCTATGAGAACAGCTGGACC AAGCTGACTGAAAGATTCTTCAAGAATACACCTTGGCCCGAGGCTGAAGCCATTGCTCCA CAGGTTGGCAATGATGCTGTCTTCCTGATTTTATACAAAGAATTATACTACAGGCACATA TATGCCAAAGTCAGTGGGGGACCTTCCTTGGAGCAGAGGTTTGAATCCTATTACAACTAC TGCAATCTCTTCAACTACATTCTTAATGCCGATGGTCCTGCTCCCCTTGAACTACCCAAC CAGTGGCTCTGGGATATTATCGATGAGTTCATCTACCAGTTTCAGTCATTCAGTCAGTAC CGCTGTAAGACTGCCAAGAAGTCAGAGGAGGAGATTGACTTTCTTCGTTCCAATCCCAAA ATCTGGAATGTTCATAGTGTCCTCAATGTCCTTCATTCCCTGGTAGACAAATCCAACATC AACCGACAGTTGGAGGTATACACAAGCGGAGGTGACCCTGAGAGTGTGGCTGGGGAGTAT GGGCGGCACTCCCTCTACAAAATGCTTGGTTACTTCAGCCTGGTCGGGCTTCTCCGCCTG CACTCCCTGTTAGGAGATTACTACCAGGCCATCAAGGTGCTGGAGAACATCGAACTGAAC AAGAAGAGTATGTATTCCCGTGTGCCAGAGTGCCAGGTCACCACATACTATTATGTTGGG TTTGCATATTTGATGATGCGTCGTTACCAGGATGCCATCCGGGTCTTCGCCAACATCCTC CTCTACATCCAGAGGACCAAGAGCATGTTCCAGAGGACCACGTACAAGTATGAGATGATT AACAAGCAGAATGAGCAGATGCATGCGCTGCTGGCCATTGCCCTCACGATGTACCCCATG CGTATTGATGAGAGCATTCACCTCCAGCTGCGGGAGAAATATGGGGACAAGATGTTGCGC ATGCAGAAAGGTGACCCACAAGTCTATGAAGAACTTTTCAGTTACTCCTGCCCCAAGTTC CTGTCGCCTGTAGTGCCCAACTATGATAATGTGCACCCCAACTACCACAAAGAGCCCTTC CTGCAGCAGCTGAAGGTGTTTTCTGATGAAGTACAGCAGCAGGCCCAGCTTTCAACCATC CGCAGCTTCCTGAAGCTCTACACCACCATGCCTGTGGCCAAGCTGGCTGGCTTCCTGGAC CTCACAGAGCAGGAGTTCCGGATCCAGCTTCTTGTCTTCAAACACAAGATGAAGAACCTC GTGTGGACCAGCGGTATCTCAGCCCTGGATGGTGAATTTCAGTCAGCCTCAGAGGTTGAC TTCTACATTGATAAGGACATGATCCACATCGCGGACACCAAGGTCGCCAGGCGTTATGGG GATTTCTTCATCCGTCAGATCCACAAATTTGAGGAGCTTAATCGAACCCTGAAGAAGATG GGACAGAGACCTTGA Clone variation with respect to NM_016091.3 >OriGene 5' read for NM_016091 unedited
NGGGTCAATATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGCAAGC GAGGCAGCCATGTCTTATCCCGCTGATGATTATGAGTCTGAGGCGGCTTATGACCCCTAC GCTTATCCCAGCGACTATGATATGCACACAGGAGATCCAAAGCAGGACCTTGCTTATGAA CGTCAGTATGAACAGCAAACCTATCAGGTGATCCCTGAGGTGATCAAAAACTTCATCCAG TATTTCCACAAAACTGTCTCAGATTTGATTGACCAGAAAGTGTATGAGCTACAGGCCAGT CGTGTCTCCAGTGATGTCATTGACCAGAAGGTGTATGAGATCCAGGACATCTATGAGAAC AGCTGGACCAAGCTGACTGAAAGATTCTTCAAGAATACACCTTGGCCCGAGGCTGAAGCC ATTGCTCCACAGGTTGGCAATGATGCTGTCTTCCTGATTTTATACAAAGAATTATACTAC AGGCACATATATGCCAAAGTCAGTGGGGGACCTTCCTTGGAGCAGAGGTTTGAATCCTAT TACAACTACTGCAATCTCTTCAACTACATTCTTAATGCCGATGGTCCTGCTCCCCTTGAA CTACCCAACCAGTGGCTCTGGGATATTATCGATGAGTTCATCTACCAGTTTCAGTCATTC AGTCAGTACCGCTGTAAGACTGCCAAGAAGTCAGAGGAGGAGATTGACTNTCTTCGTTCC AATCCCAAAATCTGGAATGTTCATAGTGTCCTCAATGTCCTTCATTCCCTGGTAGACANA TCCAACATCAACCCGACAGTGGGAGTATACACAAGCGGNAGTGACCCTGANAGTGTGGCT GGGGAGTATGGGNCGGACTCCCTCTACAAATGCTTGGTACCTCAGCCTGGTCGGNCT >OriGene 3' read for NM_016091 unedited
CGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCTGGCTCCAAATATCCTTTACTGAG ATCCACTTGAAACACTTCGGTCCTTAACTTGTTAACTGAGTTGACAGGCTGATGGCTGAT CTAGGTAAAGGTTTCACGGTAGCAAAAACACTTCCTGCCTATAATACATCAAAACAGGTT CCTGAATGTGTGTGAATATCATCAAGGTCTCTGTCCCATCTTCTTCAGGGTTCGATTAAG CTCCTCAAATTTGTGGATCTGACGGATGAAGAAATCCCCATAACGCCTGGCGACCTTGGT GTCCGCGATGTGGATCATGTCCTTATCAATGTAGAAGTCAACCTCTGAGGCTGACTGAAA TTCACCATCCAGGGCTGAGATACCGCTGGTCCACACGAGGTTCTTCATCTTGTGTTTGAA GACAAGAAGCTGGATCCGGAACTCCTGCTCTGTGAGGTCCAGGAAGCCAGCCAGCTTGGC CACAGGCATGGTGGTGTAGAGCTTCAGGAAGCTGCGGATGGTTGAAAGCTGGGCCTGCTG CTGTACTTCATCAGAAAACACCTTCAGCTGCTGCAGGAAGGGCTCTTTGTGGTAGTTGGA GTGCACATTATCATAGTTGGGCACTACAGGCGACAGGAACTTGNGGCAGGAGTAACTGAA AAGTTCTTCATAGACTTGTGGGTCACCTTTCTGCATGCGCAACATCTTGTCCNCATATTT CTCCAGCAGCTGGAGGTGAATGCTCTCATCNATACGCATGGNGTACATCGTGAAGGCAAT GGNCAGCAGCGCATGCATCTGCTCATTCTGGCTGTAATCATCTCATACTTGNACGGAGGC CTCTGGAACATGCTCTTGGTCCTCTGGATGTANAGGAAGATTGTNGCGAAAACCCGNATG CCTCCTGNTACGACGCATCTCAATATGCAAACCCACATATATATTGGTGACCTGCACTCT GCACACGGAATACTACTCTCTGTCATTCGAGTTTTCAA |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_016091 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016091.2, NP_057175.1 |
| RefSeq Size | 1970 bp |
| RefSeq ORF | 1695 bp |
| Locus ID | 51386 |
| UniProt ID | Q9Y262 |
| Gene Summary | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206690 | EIF3L (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit L (EIF3L) |
CNY 4200.00 |
|
| RC206690L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit L (EIF3L), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC206690L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit L (EIF3L), mGFP tagged |
CNY 5890.00 |
|
| RG206690 | EIF3L (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit L (EIF3L) |
CNY 4370.00 |
|
| SC323942 | EIF3L (untagged)-Human eukaryotic translation initiation factor 3, subunit L (EIF3L) |
CNY 4208.00 |
