TRM1 (TRMT1) (NM_017722) Human Untagged Clone
CAT＃: SC114002
TRMT1 (untagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1
CNY 4912.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MRT68; TRM1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114002 sequence for NM_017722 edited (data generated by NextGen Sequencing)
ATGCAAGGATCGTCTCTGTGGCTAAGCCTCACTTTCCGCTCCGCCCGGGTGCTCTCTAGA GCCCGGTTTTTCGAGTGGCAGTCTCCAGGGCTGCCGAATACAGCAGCGATGGAGAACGGC ACCGGGCCCTACGGAGAAGAACGTCCACGTGAAGTCCAGGAGACGACAGTCACCGAGGGG GCTGCCAAAATCGCCTTTCCCAGTGCCAACGAGGTCTTTTATAACCCGGTGCAGGAATTC AATCGGGACCTGACATGTGCTGTGATCACCGAGTTTGCTCGCATTCAGCTTGGGGCCAAA GGAATCCAGATCAAGGTTCCAGGAGAGAAGGACACGCAAAAAGTGGTCGTGGACTTGTCA GAGCAAGAGGAGGAAAAGGTTGAACTGAAAGAGAGTGAAAACCTGGCCTCAGGAGACCAA CCTCGCACAGCGGCCGTGGGGGAGATCTGTGAGGAAGGCCTGCATGTGCTGGAAGGCCTG GCAGCTTCAGGCCTACGTTCCATTCGATTTGCCCTAGAGGTGCCTGGGCTCAGATCTGTG GTTGCAAACGATGCCTCCACCCGGGCTGTGGATCTCATACGCCGGAATGTCCAGCTCAAT GACGTGGCCCACCTGGTACAGCCGAGCCAAGCAGATGCCCGGATGCTGATGTACCAGCAC CAGAGGGTGTCGGAGAGGTTTGACGTCATCGATCTGGACCCCTATGGCAGCCCAGCCACC TTCCTGGATGCAGCTGTGCAGGCTGNNNGTGCAGGAGGGTTGCTGTGTGTGACCTGCACA GACATGGCGGTGTTGGCGGGGAACAGCGGGGAGACGTGCTACAGCAAGTACGGGGCCATG GCCCTCAAGAGCCGGGCCTGCCACGAGATGGCCCTGAGAATCGTCCTGCACAGCCTGGAC CTCCGCGCCAACTGCTACCAGCGCTTCGTGGTGCCGCTGCTCAGCATCAGCGCTGACTTC TACGTGCGTGTTTTTGTCCGTGTCTTCACCGGCCAGGCCAAGGTCAAGGCCTCAGCCAGC AAGCAGGCGCTGGTGTTCCAGTGTGTGGGCTGCGGGGCCTTCCACCTTCAGCGTCTCGGC AAAGCGTCAGGAGTCCCCAGCGGCCGGGCCAAGTTCTCTGCAGCCTGTGGTCCCCCTGTG ACCCCCGAGTGTGAACACTGTGGGCAACGACACCAGCTTGGTGGCCCCATGTGGGCAGAG CCCATCCATGACCTGGATTTTGTGGGCCGTGTCCTGGAGGCTGTGAGCGCTAACCCCGGC CGCTTCCACACCTCGGAGCGGATCCGAGGGGTCCTGAGCGTCATCACTGAGGAGCTCCCG GACGTGCCTCTGTACTACACCCTGGACCAGCTGAGCAGCACCATCCACTGCAACACACCA AGCCTCCTGCAGTTGCGGTCGGCCCTCCTCCACGCTGACTTCCGGGTCTCACTCTCCCAC GCCTGTAAGAACGCTGTGAAGACGGATGCCCCTGCCTCTGCCCTCTGGGACATCATGCGT TGCTGGGAGAAGGAATGTCCGGTGAAACGGGAGCGACTATCAGAGACTAGCCCAGCGTTC CGCATTCTCAGTGTGGAGCCCAGGCTGCAGGCCAACTTCACCATCCGGGAAGATGCCAAC CCCAGCTCCCGACAGCGAGGACTCAAGCGCTTCCAGGCTAACCCGGAGGCCAACTGGGGT CCCCGGCCTCGTGCCCGGCCAGGGGGCAAGGCGGCCGACGAAGCTATGGAGGAGAGACGC AGGCTGCTTCAGAACAAGCGGAAGGAGCCGCCGGAAGATGTGGCCCAGCGGGCTGCCCGG CTCAAGACATTTCCTTGCAAGAGGTTTAAGGAGGGCACCTGTCAACGCGGGGACCAGTGC TGCTACTCCCACAGCCCCCCGACACCCAGGGTTTCTGCTGATGCTGCCCCTGACTGTCCA GAGACCTCCAACCAGACCCCCCCTGGACCTGGGGCTGCCGCTGGGCCAGGCATAGACTGA Clone variation with respect to NM_017722.3 746 t=>n;747 g=>n;748 a=>n;752 a=>c >OriGene 5' read for NM_017722 unedited
CACATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGNAAACGTGCA CCTTGACTAATTCGGTTTTCTGTGGCTACGAGAGCAGGCTTGGCGGGCGGAGGCGCCAGC GGATGTCTCATGCAAGGATCGTCTCTGTGGCTAAGCCTCACTTTCCGCTCCGCCCGGGTG CTCTCTAGAGCCCGGTTTTTCGAGTGGCAGTCTCCAGGGCTGCCGAATACAGCAGCGATG GAGAACGGCACCGGGCCCTACGGAGAAGAACGTCCACGTGAAGTCCAGGAGACGACAGTC ACCGAGGGGGCTGCCAAAATCGCCTTTCCCAGTGCCAACGAGGTCTTTTATAACCCGGTG CAGGAATTCAATCGGGACCTGACATGTGCTGTGATCACCGAGTTTGCTCGCATTCAGCTT GGGGCCAAAGGAATCCAGATCAAGGTTCCAGGAGAGAAGGACACGCAAAAAGTGGTCGTG GACTTGTCAGAGCAAGAGGAGGAAAAGGTTGAACTGAAAGAGAGTGAAAACCTGGCCTCA GGAGACCAACCTCGCACAGCGGCCGTGGGGGAGATCTGTGAGGAAGGCCTGCATGTGCTG GAAGGCCTGGCAGCTTCAGGCCTACGTTCCATTCGATTTGCCCTAGAGGTGCCTGGGCTC AGATCTGTGGTTGCAAACGATGCCTCCACCCGGGCTGTGGATCTCATACGCCGGAATGTC CAGCTCAATGACGTGGCCCACCTGGTACAGCCGAGCCAAGCAGATGCCCGGATGCTGATG TACCAGCACCAGNAGGTGTCGGAGAAGTTTGACGTCATCGATCTGGACCCCTATGGCAGC CCAGCCACCTTCCTGNATGCANCTGTGCANGCTGGNTGCTGTGTGTGACCTGCACGACAT GNNCGTGTTNNGCGGNGACANCGNGNAGACGTGCTACAGCAGTACGGNGCCATGGNCCTC CAGACCNGGCCTN >OriGene 3' read for NM_017722 unedited
NNNNNTTACTTGNACCCGGCCGCATNCTANNGATCGGTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTGACGTGACATCTCTTTATTGGTTCAGTCTATGCCTGGCCCAGCGGCAGCCCC AGGTCCAGGGGGGGTCTGGTTGGAGGTCTCTGGACAGTCAGGGGCAGCATCAGCAGAAAC CCTGGGTGTCGGGGGGCTGTGGGAGTAGCAGCACTGGTCCCCGCGTTGACAGGTGCCCTC CTTAAACCTCTTGCAAGGAAATGTCTTGAGCCGGGCAGCCCGCTGGGCCACATCTTCCGG CGGCTCCTTCCGCTTGTTCTGAAGCAGCCTGCGTCTCTCCTCCATAGCTTCGTCGGCCGC CTTGCCCCCTGGCCGGGCACGAGGCCGGGGACCCCAGTTGGCCTCCGGGTTAGCCTGGAA GCGCTTGAGTCCTCGCTGTCGGGAGCTGGGGTTGGCATCTTCCCGGATGGTGAAGTTGGC CTGCAGCCTGGGCTCCACACTGAGAATGCGGAACGCTGGGCTAGTCTCTGATAGTCGCTC CCGTTTCACCGGACATTCCTTCTCCCAGCAACGCATGATGTCCCAGAGGGCAAAGGCAGG GGCATCCGTCTTCACAGCGTTCTTACAGGCGTGGGAGAGTGAGACCCGGAAGTCAGCGTG GAGGAGGGCCGACCGCAACTGCAGGAGGCTTGGTGTGTTGCAGTGGATGGTGCTGCTCAN CTGGTCCAGGGTGTAGTACAGAGGCACGTCCGGGAGCTCCTGCGATGGGGGACAGGATGG GCATGAGTGGAGATGGACCGGCACAGGTGGAGCCCCCCTCCCCGTCACAGGCCGGTTCTC ACCTTAGAGATGACGCTCAGGACCCCTCGGATCCGGTCCCGAGTGTGGAAGCGCCCGGGG T |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017722 |
| Insert Size | 2640 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017722.2, NP_060192.1 |
| RefSeq Size | 2217 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 55621 |
| UniProt ID | Q9NXH9 |
| Domains | zf-CCCH, TRM |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1). |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208040 | TRMT1 (Myc-DDK-tagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 7360.00 |
|
| RC208040L3 | Lenti-ORF clone of TRMT1 (Myc-DDK-tagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 5890.00 |
|
| RC208040L4 | Lenti-ORF clone of TRMT1 (mGFP-tagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 5890.00 |
|
| RG208040 | TRMT1 (tGFP-tagged) - Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 4370.00 |
