GON4L (NM_017710) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC113993 sequence for NM_017710 edited (data generated by NextGen Sequencing) ATGACGGATGAAGCAGTTGGAGACTCTGCTGAGAAGCCTCCTACTTTTGCTTCACCTGAG ACTGCTCCAGAAGTGGAGACCAGCAGAACTCCACCAGGAGAGAGCATCAAAGCTGCTGGA AAAGGCCGGAACAATCATCGAGCTCGCAACAAGCGGGGAAGTCGGGCTCGGGCCAGCAAG GACACCTCCAAGCTGCTGTTGCTGTATGATGAGGACATTCTCGAGCGAGATCCACTCAGG GAGCAGAAGGACTTGGCCTTTGCCCAAGCTTATCTGACCAGGGTGCGAGAAGCCCTACAA CATATCCCTGGCAAGTATGAAGACTTCCTTCAAGTCATCTATGAATTTGAGTCAAGTACC CAGAGACGGACGGCTGTAGATCTCTACAAAAGCCTGCAAATTCTGCTCCAAGACTGGCCT CAGCTGTTGAAAGACTTTGCTGCTTTCCTGTTACCTGAGCAAGCTCTGGCCTGTGGATTA TTTGAGGAGCAGCAGGCTTTTGAGAAGAGCCGCAAGTTCCTTCGGCAGCTGGAGATTTGC TTTGCAGAGAACCCCTCACACCACCAGAAGATTATCAAGGTCCTCCAAGGCTGTGCAGAC TGCCTTCCCCAGGAGATCACCGAGCTCAAGACACAGATGTGGCAGCTCCTCAAGGGCCAC GACCACCTGCAGGATGAGTTTTCTATCTTCTTTGACCACTTGCGCCCAGCAGCTAGCCGG ATGGGTGACTTTGAAGAGATCAATTGGACTGAGGAAAAGGAGTATGAGTTTGATGGCTTT GAAGAAGTGGCCCTGCCTGATGTGGAAGAAGAGGAGGAGCCTCCCAAGATACCCACAGCC TCAAAGAACAAGAGGAAAAAAGAGATCGGGGTCCAAAATCATGATAAGGAGACTGAATGG CCAGATGGGGCCAAGGACTGTGCCTGCTCCTGCCATGAAGGAGGTCCAGATTCCAAGCTG AAGAAGAGCAAAAGGCGGAGCTGTAGCCACTGTAGCAGCAAGGTCTGTGACAGCAAATCC TACAAGAGCAAGGAGCCCCATGAGTTGGTGGGCAGCAGCCCCCACCGAGAGGCTAGTCCT ATGCCTGGTGCTAAGGAAGCTGGGCAGGGCAAGGATATGATGGAAGAGGAAGCCCCAGAG GAGCGGGAGAGCACTGAGGCCACCCAGAGCAGGACTGTCAGGACCACCAGAAAGGGAGAG ATGCCTGTTTCAGGATTGGCAGTGGGGAGCACTTTGCCATCCCCTCGAGAAGTGACTGTT ACAGAACGGCTCCTCCTGGATGGCCCACCACCTCATTCACCAGAGACTCCTCAATTTCCC CCCACAACTGGAGCTGTACTGTACACTGTTAAGAGAAACCAGGTTGGGCCTGAGGTTCGC TCCTGCCCCAAGGCATCCCCCAGACTTCAGAAAGAGAGGGAGGGCCAAAAGGCAGTGAGT GAGTCAGAGGCTTTGATGCTGGTCTGGGATGCATCAGAAACTGAGAAATTGCCTGGTACC GTGGAACCCCCTGCTTCCTTCCTGAGTCCTGTTTCCTCAAAGACCAGAGATGCAGGGAGA AGACATGTGTCCGGGAAACCAGACACTCAAGAGAGATGGCTGCCCTCAAGCAGAGCTCGG GTGAAGACAAGAGACAGGACGTGCCCTGTCCATGAATCTCCATCAGGAATTGACACCTCA GAGACTTCTCCCAAAGCCCCTAGAGGGGGTTTGGCTAAAGACAGTGGAACACAGGCCAAG GGTCCAGAGGGGGAGCAGCAGCCAAAGGCCGCAGAAGCTACGGTGTGTGCCAACAACAGC AAGGTCAGCTCCACTGGGGAAAAGGTTGTCCTGTGGACAAGGGAAGCTGACCGTGTGATC CTCACCATGTGCCAGGAGCAAGGGGCACAGCCACAGACCTTCAACATCATCTCCCAGCAG CTGGGAAATAAGACCCCTGCTGAGGTTTCCCACCGTTTTCGAGAACTCATGCAGCTCTTC CACACTGCCTGTGAAGCCAGCTCTGAGGATGAGGATGATGCAACCAGTACCAGCAATGCA GACCAGCTGTCTGACCATGGGGACCTTCTGTCTGAAGAGGAGCTGGATGAATGA Clone variation with respect to NM_017710.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_017710 unedited NGGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGNAATTC TCAGNAGGAGAACTCTGAGCCANAAGAAGAGAGGAAGAAGAAGCAGAAGGAATGGAAAGC CTGCAGAAAGAGGATGAAATGACGGATGAAGCAGTTGGAGACTCTGCTGAGAAGCCTCCT ACTTTTGCTTCACCTGAGACTGCTCCAGAAGTGGAGACCAGCAGAACTCCACCAGGAGAG AGCATCAAAGCTGCTGGAAAAGGCCGGAACAATCATCGAGCTCGCAACAAGCGGGGAAGT CGGGCTCGGGCCAGCAAGGACACCTCCAAGCTGCTGTTGCTGTATGATGAGGACATTCTC GAGCGAGATCCACTCAGGGAGCAGAAGGACTTGGCCTTTGCCCAAGCTTATCTGACCAGG GTGCGAGAAGCCCTACAACATATCCCTGGCAAGTATGAAGACTTCCTTCAAGTCATCTAT GAATTTGAGTCAAGTACCCAGAGACGGACGGCTGTAGATCTCTACAAAAGCCTGCAAATT CTGCTCCAAGACTGGCCTCAGCTGTTGAAAGACTTTGCTGCTTTCCTGTTACCTGAGCAA GCTCTGGCCTGTGGATTATTTGAGGAGCAGCAGGCTTTTGAGAAGAGCCGCAAGTTCCTT CGGCAGCTGGAGATTTGCTTTGCAGAGAACCCCTCACACCACCAGAAGATTATCAAGGTC CTCCAAGGCTGTGCAGACTGCCTTCCCCAGNAGATCACCGAGCTCAAGAACAGATGTGGC AGCTCCTCAAGGCCACGACCACCTGCAGGATGAGTTNTCTATCTTCTTTGACCACTTGCG CCCAGCAGCTAGCCGGATGGGTGACTCTGAAGAGATCAATTGGACTGAGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_017710 unedited GTCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAACAGTTAAACATTCC TTTATTCACCCTATGGCTCCCAAAAAAACATTCTTCCTCTGGAGTACTGGTGTACTAAGG GGACAATACACCAAATTTGTTGAGTTTACAATCAAGTCTACTAAGGTTGGACTTCCTTAT CAGTTTGGCGAGTCCCAGGGCAGAATAATCATCCATCTACAGGTCTCTGTTTCCTCTCCC TCCACAGCAGTGGAGAGCATCCCAGTGTTTGGGGCACTGTGTTCCTCTTCGTCCCTGCAC CAGACCCTGGAAGCCTTGGCCAGAGACCTCACCAAACTCGACTTGCGGCGCTGGGCCAGC TTCATGGATGCTGGAGTGGAGCACGATGACGTAGCAAAGCTGCTGCAGGAGCTACAAAGC CTGGCCCAGTGCTACCAGGGTGGTGACAGCCTCGTGGACTAAAGTTCCCAGTGTGGGAGA AAGGAGCTAGTTTGCAATAAAAACAGCTGGATGCAGGAGCCCAGTGTCTTCATGCAGAGG AGCTCAATGTCGCGGGACTAGCTACACCAACATATGCACTTTTTACATTTAGAAACACTG TGATTAGACCACAGAACAATAAATATGTGCCATCAGACCAAAAAAAAGTAGAGAAAGGAG CTGAACTCCACTCTCGATGCTATTTACAGAGGACATCTGTAAAGTCTTCATAAAAGACCT TGAATGATGCCTAGGATGGCAGAGCCCCTGGGTCCTACTCCATCCTCCAGCCTTTGTCCT TGTCCTGGCCTCCTGCTCTCCAGATCTGTAAACTGGGCTCAAGGACTGTANCAGCAGAGT ACAACTACCCCCTTCCCCGTGCCAGGCGCCCTGTNGGGTTTGGTCCTGTGTANATGATTN CCCAGATCTCATTCATTCCAGCTCTCTCAGACAGAAGGTCCCATGGTCAC
Restriction Sites	NotI-NotI
ACCN	NM_017710
Insert Size	3540 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017710.1, NP_060180.1
RefSeq Size	2994 bp
RefSeq ORF	2994 bp
Locus ID	54856
Gene Summary	Has transcriptional repressor activity, probably as part of a complex with YY1, SIN3A AND HDAC1. Required for B cell lymphopoiesis.[UniProtKB/Swiss-Prot Function]

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