NLE1 (NM_018096) Human Untagged Clone
CAT＃: SC113777
NLE1 (untagged)-Human notchless homolog 1 (Drosophila) (NLE1), transcript variant 1
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NLE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_018096, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCAGCAGTGCCGGACGAGGCGGTGGCGCGCGATGTGCAGCGGTTGCTAGTGCAGTTCCAGGATG AGGGCGGGCAGCTGCTGGGTTCCCCGTTCGACGTGCCCGTGGACATCACCCCGGACAGGCTGCAGCTCGT GTGCAACGCGCTACTGGCCCAGGAGGATCCCCTGCCACTGGCTTTCTTTGTCCACGATGCTGAGATCGTC TCCTCACTGGGGAAGACGTTGGAGTCCCAGGCAGTGGAGACAGAGAAGGTCCTAGACATCATCTACCAGC CACAGGCTATCTTCAGAGTCCGGGCTGTGACTCGCTGCACCAGCTCCTTGGAGGGTCACAGTGAGGCAGT CATTTCTGTGGCCTTCAGCCCTACGGGAAAGTACCTGGCCAGTGGCTCTGGAGACACCACCGTGCGCTTC TGGGATCTCAGCACAGAGACACCACATTTCACATGCAAGGGACACAGACACTGGGTCCTTAGTATATCCT GGTCTCCAGATGGCAGGAAGCTGGCCTCAGGCTGCAAGAATGGCCAGATTCTCCTCTGGGACCCAAGCAC AGGGAAGCAGGTGGGCAGGACCCTCGCTGGCCACAGCAAGTGGATCACAGGCCTGAGCTGGGAGCCCCTC CATGCGAACCCTGAGTGCCGCTATGTGGCCAGCAGCTCCAAGGATGGCAGTGTGCGGATCTGGGACACAA CTGCAGGCCGCTGTGAGCGCATCCTCACCGGGCACACCCAGTCGGTCACCTGTCTCCGGTGGGGAGGGGA CGGGCTTCTCTACTCTGCCTCCCAGGACCGCACCATCAAAGTCTGGAGAGCTCATGACGGTGTGCTGTGC CGGACTCTGCAAGGCCACGGCCACTGGGTGAACACCATGGCCCTCAGCACTGACTATGCCCTGCGCACTG GGGCCTTTGAACCTGCTGAGGCCTCAGTTAATCCCCAAGACCTCCAAGGATCCTTGCAGGAGTTGAAGGA GAGGGCTCTGAGCCGATACAACCTCGTGCGGGGCCAGGGTCCAGAGAGGCTGGTGTCTGGCTCCGACGAC TTCACCTTATTCCTGTGGTCCCCAGCAGAGGACAAAAAGCCTCTCACTCGGATGACAGGACACCAAGCTC TCATCAACCAGGTGCTCTTCTCTCCTGACTCCCGCATCGTGGCTAGTGCCTCCTTTGACAAGTCCATCAA GCTGTGGGATGGCAGGACGGGCAAGTACCTGGCTTCCCTACGCGGCCACGTGGCTGCCGTGTACCAGATT GCGTGGTCAGCTGACAGTCGGCTCCTGGTCAGCGGCAGCAGTGACAGCACACTGAAGGTGTGGGATGTGA AGGCCCAGAAGCTGGCCATGGACCTGCCCGGCCACGCGGATGAGGTATATGCTGTTGACTGGAGTCCAGA TGGCCAGAGAGTGGCAAGTGGTGGGAAGGACAAATGCCTCCGGATATGGAGGAGATGA >OriGene 5' read for NM_018096 unedited
GTCACAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGAGAGAGG CGAAGTGGGGCCCTTCTGGGCGGGCGACCAGACTGACTCCCGCCCCACTTGCTCGCGCAG GACGAGGCGGTGGCGCGCGATGTGCAGCGGTTGCTAGTGCAGTTCCAGGATGAGGGCGGG CAGCTGCTGGGTTCCCCGTTCGACGTGCCCGTGGACATCACCCCGGACAGGCTGCAGCTC GTGTGCAACGCGCTACTGGCCCAGGAGGATCCCCTGCCACTGGCTTTCTTTGTCCACGAT GCTGAGATCGTCTCCTCACTGGGGAAGACGTTGGAGTCCCAGGCAGTGGAGACAGAGAAG GTCCTAGACATCATCTACCAGCCACAGGCTATCTTCAGAGTCCGGGCTGTGACTCGCTGC ACCAGCTCCTTGGAGGGTCACAGTGAGGCAGTCATTTCTGTGGCCTTCAGCCCTACGGGA AAGTACCTGGCCAGTGGCTCTGGAGACACCACCGTGCGCTTCTGGGATCTCAGCACAGAG ACACCACATTTCACATGCAAGGGACACAGACACTGGGTCCTTAGTATATCCTGGTCTCCA GATGGCAAGAAGCTGGCCTCAGGCTGCAAGAATGGCCAGATTCTCCTCTGGGACCCAAGC ACAGGGAAGCAGGTGGGCAGGACCCTCGCTGGCCACAGCAAGTGGATCACAGGCCCTGAG CTGGGAGCCCCTCCATGCGAACCCTGAGTGCCGCTATGTGGCCACCAGCTCCAAGGATGG CAGTGTGCGGATCCTGGACACAACTGCAGCCCGCTGTGAGCGCATCCTCACCGGGCACAC CCAGTCGGTCACCTGTCTTCCGTTGGGGATGGGACCGGGCTTTTCTACTCTGCCTTCCAG ACCGAACCATCAAGGCTGGACAGT >OriGene 3' read for NM_018096 unedited
TTGGACAGTTTTTACAAGGGATGGTTTGGNAAATCCAANCTGAGTAGTTCAGACCATGAC CCTGCTTAGCATGGGATGCAGNGAGAAAGCAGAGGCAGGGTACATTCAGGGCAAGATCTC GGCCTGGGGTCTGAGTCCTGATGGGAATTCCTTTCACTGCTTGAAAGTTCTCGTGGCAAA GAACTTCCTCTGTATCAACAATACCCACTTCTGCTCAGAGCTCATACTGCTGCCTCCCCA TCTGAGGGAATCCTCTGGAAGAGCCATCACCTTCCCCGCTCTATCCCATGGGCCCAGGGC AGTGCTTGGTGCCTGGCAGGGCCTCAAGCAGTGTGCTATGCGAAGGCAGAGGCATCCCCT GGAGCAGGGCTGACCAGCAGAACTCTCTTGATGATGAAAACCTTTCTGTGTGCTGTCCAA TACTGGAGGCCTTACCCAGTGCGATGGAGGAACTGCACTTCTATTTCATGCCAGCAGTCA CATGTGGCCGGTGCCTACTGCAGTGGACAACAATACAGCCCTAGATTCTCCTTGGCCACT ATCACAACTGGCCTCTCTCTTACCAACACGGGGGAAGAGAGGGAAGGCAAGTGCCTGGTC AGTGTGGTTTTGGCGTAGTCCAGTGCAAGGGCGGNGGCTGGACAATCTCATTTAGCACAG TCCTTGATTGAGTCATGGGGATTCTAGGACAAGGAATTAGAACCCAACACACAAAACTTA AGTTACTTATTTGGTCATAGAACCTGNGATCTGACCCTGTACAGAGCTGACACTANGGGC CCACTCATAGAGCCTGGCTGCCCACCACCCCTGTCTGTACCCAGCAGGTACAGAGTAGCA GACACGGGCCAAGNTCAGTGACAACTTCCTGTGACCTTCGATTCTGGCTCTGGGTGTGCC ACAGCGGNGATCTGTGGGAAAACCCATTNCGTCTATGAGACAGCAGCCACGCTCTCAGTC CCACTGTGGAGGTGTGGCACTGCATTACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018096 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018096.3, NP_060566.2 |
| RefSeq Size | 2591 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 54475 |
| UniProt ID | Q9NVX2 |
| Domains | WD40 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204114 | NLE1 (Myc-DDK-tagged)-Human notchless homolog 1 (Drosophila) (NLE1), transcript variant 1 |
CNY 3656.00 |
|
| RC204114L3 | Lenti ORF clone of Human notchless homolog 1 (Drosophila) (NLE1), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC204114L4 | Lenti ORF clone of Human notchless homolog 1 (Drosophila) (NLE1), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG204114 | NLE1 (tGFP-tagged) - Human notchless homolog 1 (Drosophila) (NLE1), transcript variant 1 |
CNY 5256.00 |
